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Multi-component content determining method of eucommia ulmoides herb

A determination method and multi-component technology, which can be used in material separation, measurement devices, analysis materials, etc., can solve the problems of single determination index, difficult to comprehensively evaluate the intrinsic quality of Eucommia ulmoides medicinal materials, and no determination method is given, and achieve good repeatability. Effect

Inactive Publication Date: 2016-11-16
GUIZHOU MEDICAL UNIV
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Problems solved by technology

The 2010 edition of "Chinese Pharmacopoeia" included the determination of a single component of pinoresinol diglucoside by HPLC, but did not give the determination method of other components in Eucommia. The current quality standard content determination index is single, and it is difficult to comprehensively evaluate Eucommia medicinal materials the inner quality of

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  • Multi-component content determining method of eucommia ulmoides herb
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  • Multi-component content determining method of eucommia ulmoides herb

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Embodiment 1

[0018] 1 Instruments and materials

[0019] Acquity UPLC-PDA system (Waters Company, including binary ultra-high pressure gradient pump, autosampler, chromatographic column thermostat, array diode detector, Empower workstation); AE240 1 / 100,000 electronic balance (Mettler - Toledo Instruments Shanghai Co., Ltd.), DK-98-11 constant temperature water bath (Tianjin Test Instrument Co., Ltd.), ultra-pure water machine (Sichuan Votel Technology Development Co., Ltd.).

[0020] Geniposide reference substance, geniposide reference substance and aucubin reference substance were purchased from Sichuan Weikeqi Biotechnology Co., Ltd. (batch numbers 120314, 110722, 110711), pinoresinol diglucoside reference substance was Shanghai Shunbo Bioengineering Co., Ltd. (batch number 201206), the reference substance of pinoresinol monoglucoside was purchased from Jiangsu Honghui Pharmaceutical Co., Ltd. (batch number 070801), and the reference substance of chlorogenic acid was purchased from Beij...

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Abstract

The present invention discloses a multi-component content determining method of a eucommia ulmoides herb. The method is used for simultaneous determining the contents of aucubin, geniposidic acid, chlorogenic acid, geniposide, pinores inoldiglucoside,and pinores inolmonoglucoside in a a eucommia ulmoides herb, wherein a UPLC method is used, Waters BET C18 (2.1*150 mm, 1.7 [mu]m) is adopted as a chromatographic column, a 0.1% formic acid acetonitrile solution-0.1% formic acid aqueous solution is adopted to carry out gradient elution, the detection wavelength is 205 nm, the flow rate is 0.25 mL / min, and the column temperature is 45 DEG C. According to the method of the present invention, the six effective components in the eucommia ulmoides can be well separated, the adding recovery rate of the method is 96.92-101.01%, RSD is less than 3%, and the method can provide the basis for the quality control of the eucommia ulmoides.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a multi-component content determination method of Eucommia medicinal material. Background technique [0002] Eucommia ulmoides Eucommia ulmoides The dry bark of Oliv., also known as Sixian, Sizhong, and kapok, is a precious and nourishing medicinal material in my country. As a traditional Chinese medicine, Eucommia has a history of more than 2,000 years of medicinal use in my country. It has many functions such as nourishing the liver and kidney, strengthening bones and muscles, lowering blood pressure, anti-tumor, and preventing miscarriage. "Shen Nong's Herbal Classic" listed it as a top grade. [0003] At present, it is known that the main chemical components of Eucommia include dozens of compounds such as lignin, iridoids, phenylpropanoids, flavonoids, etc. Studies have shown that some of the active components are aucubin, pinoresin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 黄勇王爱民李勇军王永林郑林孙佳陆苑廖尚高杨畅
Owner GUIZHOU MEDICAL UNIV
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