Method of preparing pinoresinol diglucoside by endophyte and microorganism fermentation method

A technology of pinoresinol diglucoside and microbial fermentation method, which is applied in the field of preparation of pinoresinol diglucoside by utilizing endophytic bacteria and microbial fermentation method, and can solve problems such as failure to meet market demand, destruction of eucommia resources, unfavorable sustainable development, etc.

Inactive Publication Date: 2011-04-06
NORTHWEST A & F UNIV
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  • Abstract
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Problems solved by technology

This method is limited by the source of materials, cannot meet the market demand, an

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  • Method of preparing pinoresinol diglucoside by endophyte and microorganism fermentation method
  • Method of preparing pinoresinol diglucoside by endophyte and microorganism fermentation method
  • Method of preparing pinoresinol diglucoside by endophyte and microorganism fermentation method

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Embodiment Construction

[0037] Further description will be given below through specific embodiments and test examples.

[0038] 1. Isolation, purification and identification of strains

[0039] 1.1 Materials and methods

[0040] The preparation of bacterial classification XP-8: get the fresh bark of Eucommia bark, cut into the fritters of about 0.5cm * 0.5cm size, first soak in 70% alcohol for 1min, then treat with 3% sodium hypochlorite (available chlorine) solution for 2min, Wash twice with sterile water, inoculate on PDA medium plate, and culture at 25°C. Cultivate for 3-5 days. When the mycelium germinates on the plate, use separation methods such as streaking or dilution to pick colonies with different shapes, transfer them to PDA slant medium, and cultivate them at 25°C. After repeated processes of isolation, screening and purification, until the purified typical colonies are obtained. The purified strains were transferred to the slant of PDA medium for culture and stored at 4°C.

[0041] M...

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Abstract

The invention claims a method of preparing pinoresinol diglucoside by separating endophyte from Eucommia ulmoides Oliver by a microorganism fermentation method. The endophyte is XP-8 and deposited in China Center for Type Microorganism Collection under a number of CCTCCM209291. The preparation method comprises steps of preparing and fermenting liquid seeds; separating, extracting and purifying a product. The method is simple, fast and low in cost.

Description

technical field [0001] The invention relates to a method for preparing pinoresinol diglucoside, in particular to a method for preparing pinoresinol diglucoside by utilizing endophytic bacteria and microbial fermentation. Background technique [0002] Eucommia ulmoides Oliv. is a precious traditional Chinese medicine in China. Its blood pressure lowering function is continuous, stable and has no side effects, which is unmatched by other chemical blood pressure lowering drugs. Studies have shown that the main antihypertensive component in Eucommia is pinoresinol diglucoside (PDG). The traditional method of obtaining functional components in Eucommia is to extract the bark and leaves of Eucommia. This method is limited by the source of materials, cannot meet the market demand, and will destroy Eucommia resources, which is not conducive to sustainable development. Contents of the invention [0003] In order to solve the above problems, the present invention provides a method...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/44C12R1/645
Inventor 师俊玲
Owner NORTHWEST A & F UNIV
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