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Eucommia ulmoides leaf pretreatment method for extracting pinoresinol diglucoside from eucommia ulmoides leaves

A technology of pinoresinol diglucoside and eucommia leaves, which is applied in the field of pretreatment before plant extraction, and can solve the problems of drug efficacy loss, easy loss of leaf active ingredients, leaf deformation, etc.

Pending Publication Date: 2021-08-10
赵艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Finally, in the prior art, at least part of the whole treatment process is high temperature treatment, high temperature will not only cause loss of efficacy, but also partially deform leaves
For example, broken filaments at high temperature will easily cause the loss of active ingredients in the sheared leaves, and the three-dimensional structure will be destroyed, etc., which will affect the extraction in the later stage.

Method used

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  • Eucommia ulmoides leaf pretreatment method for extracting pinoresinol diglucoside from eucommia ulmoides leaves
  • Eucommia ulmoides leaf pretreatment method for extracting pinoresinol diglucoside from eucommia ulmoides leaves
  • Eucommia ulmoides leaf pretreatment method for extracting pinoresinol diglucoside from eucommia ulmoides leaves

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Experimental program
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Effect test

Embodiment 1

[0078] In this embodiment, the specific processing scheme is mainly introduced.

[0079] First, the pretreatment of Eucommia leaves:

[0080] (1) Eucommia leaves were collected from the courtyard of Xi'an Lantai Pharmaceutical Co., Ltd. in Shaanxi Province. The picking month was October. Fresh leaves with a clean surface, no wormholes, and no dryness were selected, rinsed with tap water, and stored in a freezer.

[0081] (2) Using a small vacuum freeze dryer (LGJ-18NS), dry the washed leaves at -80°C under vacuum conditions for 48 hours to obtain dry, anhydrous leaves.

[0082] (3) Cut the leaves into pieces (the particle size is about 2-3cm 2) , Freeze at -15°C for 1 h for wire breakage.

[0083] (4) Put the broken leaves into a vibrating cell-grade ultrafine pulverizer with temperature control, and pulverize at 4°C for 1 hour to obtain a brown-green powder, which is stored in a brown glass bottle away from light.

[0084] Secondly, the extraction of Eucommia leaves:

[0...

Embodiment 2

[0110] The Eucommia leaf powder in Example 1 was used as a sample, and the extraction and identification were continued.

[0111] Extraction of active ingredients from Eucommia leaves:

[0112] (1) Weigh 10.0036g of eucommia leaf powder, add ethanol solution with a mass fraction of 65%, the ratio of solid to liquid is 15mL:1g, adjust the pH of the extract to 7.4, place it in an ultrasonic cleaner, set the temperature to 70°C, and extract The time is 1.5h, and the dregs are removed by suction filtration while it is hot.

[0113] (2) Add an ethanol solution with a mass fraction of 65% to the dregs, the ratio of solid to liquid is 15mL:1g, adjust the pH of the extract to 9.1, place it in an ultrasonic cleaner, set the temperature to 70°C, and extract for 1.0h , Remove the dregs by suction filtration while hot.

[0114] (3) The two filtrates were combined, concentrated under reduced pressure to 30 mL, centrifuged for 10 min at a centrifugation speed of 8000 r / min, and the supern...

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Abstract

The invention provides a eucommia ulmoides leaf pretreatment method for extracting pinoresinol diglucoside from eucommia ulmoides leaves, and the method comprises the following steps: 1) low-temperature drying: under a vacuum freezing condition, drying fresh leaves at a first temperature T1 to obtain dried anhydrous leaves; (2) low-temperature wire breaking: shearing the dried anhydrous leaves into sheets, and freezing at a second temperature T2 for wire breaking treatment; 3) low-temperature crushing: crushing the broken leaves at a third temperature T3 to obtain brown green eucommia ulmoides leaf powder, wherein the third temperature T3 is less than 10 DEG C, and the third temperature T3 > the second temperature T2 > the first temperature T1. Through low temperature of three stages, the maintenance of effective components in the leaves is ensured.

Description

technical field [0001] The invention relates to the technical field of pre-extraction pretreatment of plants, in particular to an eucommia leaf pretreatment method for extracting pinoresinol diglucoside from eucommia leaves. Background technique [0002] Eucommia ulmoides leaves are the leaves of Eucommia ulmoides, a plant of the Eucommia family. Eucommia bark is traditionally used as medicine. It is a precious nourishing medicinal material in China since ancient times. It can nourish the liver and kidney, and strengthen the bones and muscles. For the treatment of liver and kidney deficiency, dizziness, soreness of the waist and knees, flaccidity of muscles and bones and other diseases. Modern pharmacological studies have shown that the active ingredients contained in Eucommia can regulate the immune system, endocrine system, central nervous system, circulatory system and urinary system to varying degrees, and can also stimulate the pituitary-adrenal cortex system and incre...

Claims

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Application Information

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IPC IPC(8): A61K36/46C07H15/26C07H1/08A61K127/00
CPCA61K36/46C07H15/26C07H1/08A61K2236/17A61K2236/15
Inventor 赵艳潘龙
Owner 赵艳
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