Thin-layer chromatography detection method of traditional Chinese medicine composition for increasing animal immunity

Abstract

The invention discloses a thin-layer chromatography detection method of a traditional Chinese medicine composition. The thin-layer chromatography detection method comprises the following steps: a, preparing a test solution; b, preparing a reference solution; c, respectively applying the test solution, the reference solution of astragaloside, the reference solution of oleanolic acid and the reference solution of pinoresinol diglucoside to the same silica gel plate, developing by taking the lower solution of chloroform-ethyl acetate-methanol-water as a developing agent, taking out, airing, and heating by taking 2% vanillic aldehyde sulfuric acid solution as a colour developing agent till the colour of spots is developed clearly; and d, viewing the silica gel plate after the colour is developed. The thin-layer chromatography detection method of the traditional Chinese medicine composition disclosed by the invention can be used for simultaneously detecting astragalus membranaceus, glossy privet fruits and the bark of eucommia in the traditional Chinese medicine composition; the detection result is accurate and reliable; and simultaneously, the thin-layer chromatography detection method disclosed by the invention has the advantages of being few in process, simple and convenient to operate, short in detection time, low in cost and the like and is particularly applied to industrial application.

Description

technical field [0001] The invention relates to a thin-layer chromatographic detection method of a traditional Chinese medicine composition for improving animal resistance. Background technique [0002] Thin-layer chromatography (method) is a commonly used identification method for traditional Chinese medicinal materials, which has the advantages of simple equipment, easy operation, fast separation speed, high sensitivity and resolution. [0003] Astragalus, also known as Astragalus, is the dried root of the leguminous plant Astragalus mongolica. It has the functions of nourishing qi and strengthening the surface, diuretic and swelling, detoxification and pus drainage, and muscle regeneration. It is a traditional Chinese medicine. The chemical composition of Radix Astragali is complex, mainly including saponins, flavonoids and their glycosides, polysaccharides, amino acids and so on. Ligustrum lucidum is the dry and mature fruit of Ligustrum lucidum, a plant belonging to th...

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Problems solved by technology

[0004] In the prior art, medicinal materials such as Astragalus, Ligustrum lucidum, Eucommia and their compositions are usually identified by thin-layer chromatography. However, the existing identification methods can only qualitatively identify one medicinal material in Astragalus, Ligustrum lucidum, and Eucommia ulmoides. Identification, such as: Liu Xiaoqing et al. (Liu Xiaoqing, Li Jun, Hu Jinglian, Li Xiaoyan, Qiming Kang Tingguo. Rapid TLC identification of calycosin glucoside in Astragalus medicinal materials. Journal of Liaoning University of Traditional Chinese Medicine, July 2013, Volume 15, Issue 7.) The test solution was prepared through specific pretreatment, and a specific chloroform-methanol-water (7.5:2.5:0.2) was selected as the developer to obtain detection results with clear thin-layer spots and good resolution; Zhou Yang et al. (Zhou Yang, Liu Li, Xu Desheng. TLC identification of special privet privet in decoction pieces of Ligustrum lucidum. China Pharmaceutical Industry, October 5, 2013, Volume 22, Issue 19.) Prepared by specific pretreatment test solution, and select specific ethyl acetate-acetone-formic acid-water (6:3:1:1) as the developing agent, in order to obtain the detection results with clear thin-layer spots and good resolution; Liu Ling et al. Ling Bao Jiake. TLC Identification of Eucommia Medicinal Materials. Chinese National Folk Medicine, 2013.) The test solution was prepared through specific pretreatment, and a specific chloroform-n-butanol-water-acetic acid solution was selected (1:7:1:1) as the developing agent can obtain the detection results with clear thin-layer spots and better resolution. The screening test of the developing agent has proved that even a small adjustment of the amount of each component in the developing agent will affect the detection results. The test results have a very large impact

[0005] Chinese literature (Zhai Shaoqin, Cao Guowen, Li Chengjun. TLC identification of compound golden granules. Journal of Chinese Veterinary Medicine, 2010, No. 6.) discloses the thin-layer identification method of compound golden granules, which requires the identification of astragalus, Ligustrum lucidum, etc. Different pretreatments were used to prepare the test solution, different developing agents were used, and thin-layer chromatography was carried out respectively. This method cannot carry out pretreatment and thin-layer chromatography on components such as Astragalus membranaceus and Ligustrum lucidum in compound granules at the same time. Inspection, there are many defects such as many procedures, complicated operation, long inspection time and high cost

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