37 results about "Rhynchophylline" patented technology

The invention discloses a method for online detecting the near infrared spectrum of active ingredients of compound eucommia bark capsules. The active ingredients of the compound eucommia bark capsules mainly comprise pinoresinol diglucoside, baicalin, rhynchophylline, isorhynchophylline, and the like. The method comprises the steps of collecting the near infrared absorption spectrum of the compound eucommia bark capsules, associating active ingredients content data obtained by an HPLC (High Performance Liquid Chromatography) method with the near infrared spectrum of a sample, establishing a correction model by a partial least squares cofinal method and realizing quick online detection of the active ingredients of the capsules. Research results show that the near infrared spectrum analysis method can effectively detect the active ingredients of the compound eucommia bark capsules, has simple sample, quick detection and multiple components detection at the same time, can be used for real-time analysis and online quality control during pharmaceutical processing of the traditional Chinese medicine for controlling the quality of medicines in all dimensions, enhances production automation and informatization level of traditional Chinese medicine and promotes the development of the pharmaceutical industry.

The present invention provides a preparation method for high purity rhynchophylline. The method comprises: crushing rhynchophylline medicinal material to have a particle size of 20-60 meshes; adding the crushed rhynchophylline to a sodium carbonate solution, wherein the amount of the sodium carbonate solution is 4-5 times the amount of the crushed rhynchophylline, and the sodium carbonate content in the sodium carbonate solution is 5%; carrying out ultrasonic extraction for 1 hour, and removing liquid through filtering; adding dichloromethane to carry out ultrasonic extraction for 2-3 times, recovering the regent from the extracting solution; concentrating the extracting solution to small volume; carrying out separation through high speed countercurrent chromatography, carrying out online monitoring through an ultraviolet detector; collecting the target component; carrying out cold preservation and crystallization to obtain the high purity rhynchophylline. With the method provided by the present invention, the separation treatment is performed through the high speed countercurrent chromatography; the production period is short; the product yield is high; the product purity is more than 95%.

The invention belongs to the technical field of medicine detection and particularly relates to a method for quickly detecting ramulus uncariae cum uncis by utilizing near-infrared spectrometry and application of the method. The method comprises the following steps: (1) crushing the selected ramulus uncariae cum uncis with known content of moisture, extract, rhynchophylline and isorhynchophylline, and sieving for later use; (2) collecting near infrared spectrum data of the ramulus uncariae cum uncis powder; (3) carrying out correlation on the selected spectral information under a characteristic wave band and the known content of moisture, extract, rhynchophylline and isorhynchophylline of ramulus uncariae cum uncis, and establishing a quantitative calibration model by adopting a partial least square method; and (4) performing near infrared spectrum scanning on an unknown ramulus uncariae cum uncis sample according to the method in the step (2), and introducing the selected spectral information under the characteristic wave band into the established quantitative calibration model to obtain the content values of the moisture, the extract, the rhynchophylline and the isorhynchophylline of the unknown ramulus uncariae cum uncis sample. According to the method for quickly detecting ramulus uncariae cum uncis by utilizing near-infrared spectrometry and the application of the method disclosed by the invention, quick and complete detection on ramulus uncariae cum uncis is realized by introducing a near-infrared spectrometry technology. Moreover, the method has the advantages of simplicity in operation, high accuracy and high precision.

The invention relates to a drug of indole alkaloid, and particularly relates to application of rhynchophylline in preparation of adrug with an anxiolytic effect and an antidepressant effect. The drug disclosed by the invention is composed of the rhynchophylline and pharmaceutical acceptable auxiliary materials, wherein the content of the rhynchophylline is 1-99.5%. The drug disclosed by the invention is oral preparations and can be tablets, capsules or the like.

The invention relates to a process for extracting rhynchophylline monomers from uncaria rhynchophylla. The process comprises the following steps of: pretreating uncaria rhynchophylla medicinal materials by adopting a lower alcohol water quenching ultrasonic method to prepare a crude extract; separating rhynchophylline in the crude extract by adopting a silicagel column chromatography method by using two organic reagents and ammonia water according to a certain proportion as an eluent; and finally obtaining rhynchophylline monomers through steps of eluting fractions, extracting, purifying, and the like. The method has the advantages of safety, high efficiency, low cost and high rhynchophylline monomer productivity; and per kilogram of the medicinal materials can be used for obtaining 0.27g rhynchophylline monomers, and the purity is higher than 90 percent, so that the method is beneficial to industrial large-scale production and provides an approach for the further development of rhynchophylline as a new drug.

The invention discloses a method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules. The method comprises the following steps: (1) preparing a mixed reference: weighing an appropriate amount of rhynchophylline and isorhynchophylline, accurately weighing, and adding methanol to prepare the mixed control of the rhynchophylline and the isorhynchophylline;(2) preparing a test: accurately weigh the Xiao'er Qixingcha granules, adding hot water for dissolving, carrying out ultrasonic treatment, standing for cooling, adjusting the pH value with ammonia water, then shaking and extracting with ethyl acetate, recovering the ethyl acetate liquid, and adding the methanol for dissolving residues; (3) determining by using a high performance liquid chromatography: using octadecyl silane bonded silica gel as a chromatographic column of a filling agent; mobile phase: acetonitrile (A): 0.02-0.07% diethylamine-(7-10) mM ammonium acetate (B) = (40-60) to (60-40) V / V; flow rate: 0.8-1.2mL / min; elution time: 35-60 minutes; column temperature: 25-35 DEG C; detection wavelength: 240-250nm. The content determination method is simple to operate, and can accurately reflect the real content of a product, thus being suitable for industrial daily production inspection.

The invention belongs to the field of natural medicine chemistry, and particularly relates to a preparation method of isorhynchophylline. The method comprises the following steps: 1) crushing an uncaria medicinal material, acidifying the material by 1% hydrochloric acid, performing reflux extraction with 70-80% ethanol for 2-3 times, concentrating and ammonifying the extract to obtain a pH of 9-10, standing and precipitating to obtain a precipitate; 2) separating the precipitate by high speed countercurrent chromatography, performing on-line monitoring by an ultraviolet detector, collecting the object components, performing refrigeration and crystallization to obtain high-purity rhynchophylline. The preparation method of isorhynchophylline of the invention has a simple process, easy operations, a short preparation period, and high product purity, and is applicable to continuous production.

The invention provides a cigarette additive. The cigarette additive is prepared from rhynchophylline, gastrodine or gastrodine derivatives and inonotus obliquus, the rhynchophylline, the gastrodine orthe gastrodine derivatives and the inonotus obliquus are made into a mixture, the mixture is sprayed on tobacco shreds or added into electronic cigarette accessories, and prepared cigarettes can effectively relieve the problems such as heart addiction, depression and anxiety emotion and poor sleep of meth addicts. In addition, the amount of the rhynchophylline, the gastrodine or the gastrodine derivatives and the inonotus obliquus added in the cigarettes is strictly controlled, on the one hand, the economical burden brought to consumers due to the high cost can be avoided, on the other hand,the original flavor of the cigarettes can further be ensured, and compliance of patients is good.

The invention discloses a planting method of uncaria rhynchophylla and relates to the technical field of Chinese herbal medicine planting. The planting method includes the steps of: collecting seeds, threshing and storing the seeds, processing the seeds, sowing the seeds, transplanting small seedlings, performing nursery management, performing final singling of seedlings and middle-stage management, and harvesting the uncaria rhynchophylla. In the method, the uncaria rhynchophylla sprouts in 3-4 days after sowing the seeds, so that the method greatly reduces the growth period than conventional sowing methods, in which budding period lasts for 3 months from sowing the seed in the last ten-days of March to sprouting in the middle ten-days of June. Meanwhile, the method can enrich magnetotactic microorganisms, which are key factors for stimulating sprouting of seeds and reinforcing seedlings, and establish an abundant microelement substance basis, so that the plants can be normally harvested with no need for a period of 3 years. Contents of active components, such as rhynchophylline and isorhynchophylline, in the uncaria rhynchophylla is not less than those in plants in a conventional planting method, even wild plants; according to the planting method, the seeds are sowed in spring and the plants are harvested in autumn in the third year, planting period lasting for about two and a half years; the method solves a problem of long planting period that the uncaria rhynchophylla can only be normally harvested after being planted for 3 years.

The invention discloses a granule used for syndromes after brain injuries and a preparation method thereof.The granule used for syndromes after brain injuries is prepared from, by weight, diosgenin, isorhamnetin, coumarona odoruta, kaempferol, coral nano powder, rhynchophylline, astragaloside, cerebroprotein hydrolysate, tolperisone hydrochloride, limonene, taxifolin, gamma-aminobutyric acid, glutamic acid, oryzanol, a filling agent, an adhesive, a disintegrating agent and a lubricating agent.Various medicine effective components are combined, by means of a reasonable ratio, the granule has the functions of easing pain and calming the nerves, relieving restlessness and strengthening the brain, calming the heart and nourishing qi, activating blood circulation to remove blood stasis, tonifying brain nerves and preventing fatigue, and symptoms caused when the syndromes after brain injuries occur can be relieved.Besides, brain nerve nutrient can be enhanced, symptomatic treatment and conditioning are combined, pain of a patient is relieved, mood can be relieved, and the treatment effect of healing is achieved.

The invention discloses a method of determining the content of total alkaloid in an uncaria macrophylla extract. The method is high-performance liquid chromatography with isorhynchophylline, rhynchophylline, corynoxine B and corynoxine as contrast and with methanol-0.02% triethylamine=55-75 to 25-45 as a mobile phase. The total alkaloid in the uncaria macrophylla extract is selected as an index for content determination, and the determining method has the advantages that the precision is high, the reproducibility is good, the stability is good, the determined result has high accuracy, the quality of the uncaria macrophylla extract can be effectively controlled, and the clinical use is ensured to be safe and effective.

The invention relates to a method for preparing a high-purity rhynchophylline monomer and belongs to the technical field of separation and purification of active ingredients of a traditional Chinese medicine. The method comprises the following steps: a, loading a rhynchophylline extract sample on a silica gel chromatographic column, eluting with a halogenated alkane-alcohol system, collecting eluate and concentrating to obtain a rhynchophylline crude product; and b, loading the rhynchophylline crude product dissolving solution on high-speed countercurrent chromatograph to respectively obtain a rhynchophylline monomer and a corynoxeine monomer. The method has the advantage that two monomers, namely, rhynchophylline and corynoxeine each of which the purity is more than 96% can be effectively prepared.

The invention discloses a process method for extracting rhynchophylline from uncaria rhynchophylla. The process method comprises the steps that the uncaria rhynchophylla is pretreated, mixing grinding is carried out through the micro cutting interaction technology; ethanol extraction is carried out in a single-stage continuous countercurrent ultrasonic extractor; a sample is fed to a resin chromatographic column; eluant is dried, and the rhynchophylline is obtained. Compared with the prior art, the rhynchophylline is extracted with the assistance of the micro cutting assisted interaction technology, the solubility of the rhynchophylline in an ethanol solution can be effectively improved in a submicron powder state, and the eduction rate of the rhynchophylline is further increased. In the rhynchophylline ethanol extraction process, under the action of ultrasonic waves, the continuous countercurrent extraction technology is adopted in assistance, the permeating dissolving and dispersing speed in the rhynchophylline extraction process can be increased, the extraction time is shortened, the use amount of a solvent can be reduced, and the method for extracting rhynchophylline is high in efficiency and low in consumption.

The invention relates to a process for extracting rhynchophylline monomers from uncaria rhynchophylla. The process comprises the following steps of: pretreating uncaria rhynchophylla medicinal materials by adopting a lower alcohol water quenching ultrasonic method to prepare a crude extract; separating rhynchophylline in the crude extract by adopting a silicagel column chromatography method by using two organic reagents and ammonia water according to a certain proportion as an eluent; and finally obtaining rhynchophylline monomers through steps of eluting fractions, extracting, purifying, andthe like. The method has the advantages of safety, high efficiency, low cost and high rhynchophylline monomer productivity; and per kilogram of the medicinal materials can be used for obtaining 0.27grhynchophylline monomers, and the purity is higher than 90 percent, so that the method is beneficial to industrial large-scale production and provides an approach for the further development of rhynchophylline as a new drug.

The invention relates to a new application of a medicine and particularly relates to an application of rhynchophylline in preparation of a medicine for resisting cerebral vascular contraction.

The invention belongs to the field of pharmaceutical preparations, and discloses a rhynchophylline temperature-sensitive gel nasal delivery preparation which is formed by dispersing a rhynchophylline solution in a temperature-sensitive gel matrix, every 100 mL of the rhynchophylline temperature-sensitive gel nasal delivery preparation contains 1 to 6 g of rhynchophylline, 17 to 22 g of poloxamer 407, 0.5 to 4 g of poloxamer 188, 0.5 to 3 g of polyethylene glycol 6000, 1 to 4 g of hydroxypropyl-beta-cyclodextrin and the balance of water. The rhynchophylline temperature-sensitive gel nasal delivery preparation is in a liquid state when the environment temperature does not reach the phase-change temperature, and can be changed into a semi-solid gel state from the liquid state within a short time after entering the nasal cavity, so that administration can be facilitated, and the medicine can be fully absorbed. The rhynchophylline temperature-sensitive gel nasal delivery preparation has a slow release property during drug release, can bypass a blood brain barrier, has a certain brain targeting property, and can improve the concentration of the drug in the brain.

The invention provides application of an uncaria active compound in preparation of a medicine for preventing and / or treating mental disorder diseases, and relates to the technical field of medical application. The mental disorder diseases comprise neurological and mental disorders caused by traumatic memory. The uncaria active compound disclosed by the invention can comprise rhynchophylline, isorhynchophylline, and derivatives, isomers and the like obtained by modifying molecular structures of the active compounds. The active compound provided by the invention can promote fading of wound memory, and has prevention and alleviation effects on neuropsychiatric disorders (such as post-traumatic stress disorder and phobia) and the like caused by the wound memory.

The invention relates to a method for preparing a high-purity rhynchophylline monomer and belongs to the technical field of separation and purification of active ingredients of a traditional Chinese medicine. The method comprises the following steps: a, loading a rhynchophylline extract sample on a silica gel chromatographic column, eluting with a halogenated alkane-alcohol system, collecting eluate and concentrating to obtain a rhynchophylline crude product; and b, loading the rhynchophylline crude product dissolving solution on high-speed countercurrent chromatograph to respectively obtain a rhynchophylline monomer and a corynoxeine monomer. The method has the advantage that two monomers, namely, rhynchophylline and corynoxeine each of which the purity is more than 96% can be effectively prepared.

The invention relates to an antimicrobial-free selenium-enrichment piglet mixed feed additive preparation method. A formula of the antimicrobial-free selenium-enrichment piglet mixed feed additive comprises the ingredients by weight percent: 1.8 to 2.8% of L-tyrosine, 2.3 to 3.3% of natural vitamin E, 1.2 to 2.2% of rhizoma pinelliae, 2.3 to 3.3% of lactobacillus buchneri, 1.5 to 2.5% of trisodiummonohydrogen, 2.5 to 3.5% of saw palmetto extractive, 2 to 2.8% of bacillus laterosporus, 1.3 to 2.3% of mile swertia herb, 2.6 to 3.6% of radix berberidis, 1 to 2% of enterobacter cloacae, 0.5 to 1.5% of calcium propionate, 1.2 to 2.2% of radix angelicae sinensis, 0.3 to 1.3% of dimethyl ancymidol menadione sulfite, 0.8 to 1.8% of bacillus lentus, 0.9 to 1.7% of manganese carbonate, 0.6 to 1.6%of snake gall extractive, 0.9 to 1.8% of chromium yeast, 1.2 to 2.2% of rhynchophylline, 0.9 to 1.9% of probiotic, 0.6 to 1.6% of selenium yeast and 73.6 to 54.1% of water. The antimicrobial-free selenium-enrichment piglet mixed feed additive disclosed by the invention has better functions of promoting mitosis and differentiation proliferation of lymphocyte and macrophage, increasing cell factorsand maintaining intestinal mucosa completeness and mammary epithelial cells.

The invention discloses an uncaria sustained-release tablet and a preparation method thereof; the uncaria sustained-release tablet comprises the following components in parts by weight: 1 to 30 parts of rhynchophylline or uncaria alkaloid, 10 to 70 parts of sustained-release auxiliary materials and other auxiliary materials: 0.2 to 5 parts of pore-foaming agent, 10 to 45 parts of filler, 0.5 to 20 parts of adhesive, 5 to 15 parts of wetting agent, 0.1 to 2 parts of lubricant, 0 to 5 parts of solubilizer, 0 to 10 parts of emulsifier, 0 to 25 parts of membrane material, 0 to 15 parts of opacifying agent and 0 to 25 parts of solvent. The invention slowly releases the medicine through a sustained-release technology, can effectively reduce the fluctuation of plasma concentration, prevents the 'peak and valley' phenomenon of common preparation caused by frequency medication, improves the utilization rate of the medicine, reduces the medical expenses, reduces the direct stimulation of the medicine to the gastrointestinal tract so as to reduce the side effect, so that a patient is more tolerant, thereby facilitating long-termed curing and more facilitating the curing of hypertension.

The invention relates to novel application of a medicine, in particular to application of Rhy (rhynchophylline) to preparation of medicines for improving vascular endothelial dysfunction. Incubation of renal arteries of SHRs (spontaneously hypertensive rats) is realized by application of the rhynchophylline, an improvement function of the rhynchophylline to endothelium-dependent vasodilation of the renal arteries is assessed by a multi-channel small vessel tension tester, and by which approach the rhynchophylline gives play to an improvement effect on renal vascular endothelial vasodilation dysfunction associated with hypertension is detected by a western blotting method and an NO detection kit. According to researches, the endothelium-dependent vasodilation of the renal arteries of the SHRs can be improved by incubation with the rhynchophylline in time and concentration dependency, and the improvement function of the rhynchophylline is realized by activating a Src-PI3K / Akt-eNOS passage to improve eNOS activity.

A rhynchophylline preparation process includes: (1) soaking ground Ramulus Uncariae cum Uncis in ethyl alcohol and hydrochloric acid mixed solution; (2) heating and concentrating extract liquid to a non-alcohol state, cooling, adjusting PH to 8-10, adding chloroform, and extracting to obtain a low-content rhynchophylline crude product; (3) preliminarily separating through normal-phase silica gel chromatography to obtain a high-content rhynchophylline crude product; (4) dissolving the rhynchophylline crude product in hydrochloric acid solution, and adopting a reverse-phase C18 medium-pressure column for purification to obtain high-purity rhynchophylline hydrochloride; (5) reducing the rhynchophylline hydrochloride into rhynchophylline, filtering after separating out, washing off excessive ammonium hydroxide and salt with pure water, and directly performing freeze drying to obtain a high-purity rhynchophylline product. The rhynchophylline preparation process has advantages that (1) organic reagent extraction is mostly avoided, cost is reduced, and harms of organic reagents are reduced; (2) stability of rhynchophylline in a preparation process is guaranteed, and the target yield is increased; (3) the high-purity product is obtained.

The invention discloses an ointment for treating acne, which is prepared from the following raw materials in parts by weight: 20 to 40 parts of frond of endarachne binghamiae, 30 to 50 parts of hedgeglorybind root, 15 to 22 parts of pepperweed seed, 5 to 15 parts of fruit of cerasus humilis, 10 to 16 parts of rhynchophylline, 11 to 16 parts of winterberry euonymus herb, 3 to 8 parts of phoenix tree leaf, 2 to 6 parts of wolfberry fruit extract and 10 to 15 parts of fructo-oligosaccharide. By compounding the raw materials to exert a synergistic effect, the ointment disclosed by the invention can effectively treat adolescent acne, repair scars and promote skin regeneration, the treatment period is short, and the curative effect is good; an external application method is adopted, toxic and side effects are minor, the treatment cost is reduced, and the ointment can help protect the physical and metal health of teenagers.

The present invention discloses a hypotensive pharmaceutical composition. The composition is prepared by dissolving daidzein, honokiol, soybean phosphatidylcholine, cholesterol, DSPE-PEG in an organicsolvent, then preparing a liposome suspension by a membrane-sonic method, then adding hydrochlorothiazide, rhynchophylline and a diluent into the liposome suspension, performing freeze-dying, uniformly mixing the dried material with a disintegrant and a lubricant, and finally performing tabletting. The invention uses the daidzein and the honokiol to prepare the encapsulated liposome so as to delay the release rate of the drug, and the liposome can be released simultaneously with the hydrochlorothiazide and the rhynchophylline, thereby improving the coordination of the action of drug components in the body and increasing the curative effect.

The invention relates to a method for extracting a vasodilatation active compound rhynchophylline. The method comprises the following steps: (1), grinding the raw materials and sieving to obtain a raw material powder, adding the raw material powder into an organic solvent, importing carbon dioxide to form a swelling solvent system, and stirring and extracting to obtain an extracting solution; (2), concentrating the extracting solution to obtain a primarily extracted concentrate; (3), adding an inorganic matter solution and an organic solvent in the primarily extracted concentrate to carry out liquid-liquid extraction, layering to obtain an organic phase I, adding water into the organic phase I, stirring uniformly, layering to obtain an organic phase II and concentrating the organic phase II to obtain an extract; (4), dissolving the extract and filling to the top of a chromatography column, carrying out solvent elution, collecting the eluant, concentrating to obtain a crude product, and recrystallizing to obtain the rhynchophylline. According to the method, the obtained rhynchophylline is favorable in yield and purity, has a favorable application prospect and is potential in industrial production.

The invention relates to a drug of indole alkaloid, and particularly relates to application of rhynchophylline in preparation of adrug with an anxiolytic effect and an antidepressant effect. The drug disclosed by the invention is composed of the rhynchophylline and pharmaceutical acceptable auxiliary materials, wherein the content of the rhynchophylline is 1-99.5%. The drug disclosed by the invention is oral preparations and can be tablets, capsules or the like.

The present invention discloses a hypotensive pharmaceutical composition. The composition is prepared by dissolving daidzein, honokiol, soybean phosphatidylcholine, cholesterol, DSPE-PEG in an organicsolvent, then preparing a liposome suspension by a membrane-sonic method, then adding hydrochlorothiazide, rhynchophylline and a diluent into the liposome suspension, performing freeze-dying, uniformly mixing the dried material with a disintegrant and a lubricant, and finally performing tabletting. The invention uses the daidzein and the honokiol to prepare the encapsulated liposome so as to delay the release rate of the drug, and the liposome can be released simultaneously with the hydrochlorothiazide and the rhynchophylline, thereby improving the coordination of the action of drug components in the body and increasing the curative effect.

The invention discloses a Chinese medicine active component composition capable of relaxing blood vessels. The composition comprises active components including pinoresinol diglucoside, rhynchophylline, leonurine, selfheal saponin and baicalin extracted from five common blood pressure reducing Chinese medicines including eucommia bark, uncaria, leonurus, selfheal and radix scutellariae, and the weight ratio of the pinoresinol diglucoside to the rhynchophylline to the leonurine to the selfheal saponin to the baicalin is 12:8:8:1:2. The composition can remarkably relax rat thoracic aorta vasoconstriction caused by norepinephrine, and can reduce spontaneously hypertensive rat (SHR) tail systolic arterial pressure. As the five Chinese medicines are clinical common blood pressure reducing medicines and are compatible with compound eucommia bark tablets recorded in the sixth volume of Chinese medicine component preparations from drug standards in department of health, the Chinese medicine active component composition capable of relaxing the blood vessels can serve as a clinical blood pressure reducing medicine.

The invention provides a traditional Chinese medicine gel plaster for relieving pain and a preparation method thereof, and belongs to the field of medical configuration products. The provided gel plaster includes a traditional Chinese medicine extractant, a stabilizer, an absorbent, a sodium carboxymethylcellulose, cellulose nanocrystal and inorganic calcium salt; the traditional Chinese medicineextractant consists of ramulus uncariae cum uncis, mitragyna inermis, common rush and lilac pink herb; the stabilizer consists of cucurbituril and animal protein; and the absorbent is an acylated rutin / chitosan compound. The main component of the provided gel plaster is alkaloid, the most representative of the gel plaster is rhynchophylline, and the 24-hour skin permeation rate of the rhynchophylline can reach up to 35.38%; and the provided gel also has good mechanical properties, and the fracture stress can reach up to 240.4 kPa.

The invention discloses application of rhynchophylline in preparation of a medicine for treating inflammatory enteritis. The invention firstly finds that the rhynchophylline has a repairing effect on injury caused by inflammatory enteritis; research finds that rhynchophylline can significantly reduce the concentration of inflammatory factors in colon, protect colon tissue, repair intestinal barrier permeability and activate AhR, so that intestinal inflammation can be relieved, and the intestinal barrier permeability can be repaired. The invention provides an application prospect of rhynchophylline in preparation of medicines for treating inflammatory enteritis.