Method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules

A technology of Pediatric Seven Star Tea and isorhynchophylline, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of few detection methods, complex components, and poor separation of Uncaria alkaloids, and achieve good economic and social benefits , simple pre-treatment, good separation effect

Active Publication Date: 2018-10-09
GUANGZHOU WANGLAOJI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of the drug, there are very few methods for the detection of rhynchophylline in Xiaoer Qixing tea granules. The reported methods mainly determine the content of rhynchophylline with low sensitivity and poor separation. The method report of rhynchophylline cannot comprehensively evaluate the quality of Uncaria in ingredients, and it is difficult to apply to the detection of rhynchophylline and isorhynchophylline in Xiaoer Qixing tea granules

Method used

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  • Method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules
  • Method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules
  • Method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1. Determination method of content: Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2015 edition general rule 0512).

[0059] See Table 2 and 3 for medicines and instruments

[0060] Table 2: Instruments used in this experiment

[0061]

[0062] Table 3: Reagents used in this experiment

[0063]

[0064] 2. The chromatographic conditions and system adaptability test for the determination of rhynchophylline and isorhynchophylline in Xiaoerqixingcha granules by HPLC are as follows.

[0065] (1) Preparation of mixed reference solution

[0066] Weigh an appropriate amount of rhynchophylline (purity greater than 99.0%, Shanghai Tongtian Biotechnology Co., Ltd.) and isorhynchophylline (purity greater than 99.0%, China Institute for Food and Drug Control), accurately weigh, add methanol to prepare Uncaria Alkaline and isorhynchophylline mixed control solution.

[0067] (2) preparation of need testing solution

[0068] Accurat...

Embodiment 2

[0114] (1) Preparation of mixed reference solution

[0115]Weigh an appropriate amount of rhynchophylline (purity greater than 99.0%, Shanghai Tongtian Biotechnology Co., Ltd.) and isorhynchophylline (purity greater than 99.0%, China Institute for Food and Drug Control), accurately weigh, add methanol to prepare Uncaria Alkaline and isorhynchophylline mixed control solution.

[0116] (2) preparation of need testing solution

[0117] Accurately weigh 28g of Pediatric Seven Star Tea granules, put them in a conical flask with a stopper, add 84mL of hot water at 70°C to dissolve, ultrasonicate for 30min, let cool, adjust the pH value to 9-10 with ammonia water, and then use 84mL of ethyl acetate Shake and extract once, combine and recover the ethyl acetate solution, add 1 mL of methanol to the residue to dissolve, and filter through a 0.45 μm microporous membrane to obtain the test solution.

[0118] (3) Determination by high performance liquid chromatography

[0119] Precisely...

Embodiment 3

[0129] (1) Preparation of mixed reference solution

[0130] Weigh an appropriate amount of rhynchophylline (purity greater than 99.0%, Shanghai Tongtian Biotechnology Co., Ltd.) and isorhynchophylline (purity greater than 99.0%, China Institute for Food and Drug Control), accurately weigh, add methanol to prepare Uncaria Alkaline and isorhynchophylline mixed control solution.

[0131] (2) preparation of need testing solution

[0132] Accurately weigh 28g of Pediatric Seven Star Tea granules, place them in a conical flask with a stopper, add 140mL of 80°C hot water to dissolve, ultrasonicate for 90min, let cool, adjust the pH value to 9-10 with ammonia water, and then use 140mL of ethyl acetate Shake and extract 3 times, combine and recover the ethyl acetate solution, add 1 mL of methanol to the residue to dissolve, and filter through a 0.45 μm microporous membrane to obtain the test solution.

[0133] (3) Determination by high performance liquid chromatography

[0134] Prec...

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Abstract

The invention discloses a method for determining contents of rhynchophylline and isorhynchophylline in Xiao'er Qixingcha granules. The method comprises the following steps: (1) preparing a mixed reference: weighing an appropriate amount of rhynchophylline and isorhynchophylline, accurately weighing, and adding methanol to prepare the mixed control of the rhynchophylline and the isorhynchophylline;(2) preparing a test: accurately weigh the Xiao'er Qixingcha granules, adding hot water for dissolving, carrying out ultrasonic treatment, standing for cooling, adjusting the pH value with ammonia water, then shaking and extracting with ethyl acetate, recovering the ethyl acetate liquid, and adding the methanol for dissolving residues; (3) determining by using a high performance liquid chromatography: using octadecyl silane bonded silica gel as a chromatographic column of a filling agent; mobile phase: acetonitrile (A): 0.02-0.07% diethylamine-(7-10) mM ammonium acetate (B) = (40-60) to (60-40) V / V; flow rate: 0.8-1.2mL / min; elution time: 35-60 minutes; column temperature: 25-35 DEG C; detection wavelength: 240-250nm. The content determination method is simple to operate, and can accurately reflect the real content of a product, thus being suitable for industrial daily production inspection.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a quality control method of Xiaoer Qixing tea granules. Background technique [0002] Pediatric Seven Star Tea Granules is a traditional folk herbal tea for eliminating stagnation in children. Smooth, short red urine. This variety is included in the 2015 edition of "Chinese Pharmacopoeia". The identification of hawthorn and licorice are identified under the identification item of the standard; the content determination of glycyrrhizic acid under the content determination item cannot fully evaluate the quality of this variety, and the standard is yet to be developed. improve. Among them, Uncaria has the functions of clearing away heat and calming convulsions, calming liver and quenching wind, and is the main drug of this product to stabilize convulsions. Rhynchophylline and isorhynchophylline are one of the main alkaloids of Uncaria uncaria, the two account ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 方广宏郑荣波黄晓丹吴悦和海龙林德晖
Owner GUANGZHOU WANGLAOJI PHARM CO LTD
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