The invention relates to a method for analyzing the content of an A beta plaque developer precursor AV45, belonging to the technical field of apparatus analysis. An 18F-AV45 is capable of identifying an A beta plaque pathological state in the brain of an AD (Alzheimer Disease) patient in vivo, is used for visualized and quantitative research of an amyloid protein deposition process of a human body, and moves towards the clinical. A high-purity precursor AV45 needs to be obtained if a higher radiochemical purity PET (Positron Emission Tomography) developer 18F-AV45 is obtained. According to the method, the chromatography condition is as follows: a WATERS 600 type high performance liquid chromatograph, a 2487 ultraviolet detector, a chromatographic column Inertsil C18 column, 5 micrometers and 4.6*250mm; a flowing phase is as follows: a ratio v / v of 50mM disodium hydrogen phosphate to acetonitrile is 15-85, and 0.01 percent triethylamine is added; the flowing speed is 0.8mL / min; the AV45 sample feeding volume is 10mu L; the column temperature is the room temperature; and the detection wavelength is 325nm. According to the method, phosphate is used as a buffering agent, and the triethylamine is added, so that the trailing phenomenon is improved, and the peak symmetry is better. Compared with a literature method, the method has the advantages that the number of theoretical plates and the separation degree are greatly improved, and all impurities are separated well. Various impurities can be effectively detected by using 325nm detection.