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A kind of extraction method of vasodilator active compound rhynchophylline

A technique for dilating blood vessels and an extraction method, which is applied in the field of extracting rhynchophylline, an active compound that relaxes blood vessels, and the extraction of plant active compounds, can solve the problems of low extraction rate and low purity, and achieve great technical advantages, excellent technical effects, The effect of excellent extraction rate and purity

Active Publication Date: 2016-08-24
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] As mentioned above, a variety of rhynchophylline extraction methods are disclosed in the prior art, but there are still some defects in these extraction methods, such as low extraction rate, low purity, etc.

Method used

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  • A kind of extraction method of vasodilator active compound rhynchophylline
  • A kind of extraction method of vasodilator active compound rhynchophylline
  • A kind of extraction method of vasodilator active compound rhynchophylline

Examples

Experimental program
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Effect test

preparation example 1

[0052] Preparation Example 1: Preparation of Alkaline Silica Gel

[0053] (I) Put the neutral silica gel with a particle size of 200 mesh in a muffle furnace and bake at a high temperature of 550°C for 15 hours, then lower the temperature to 200°C and keep it at this temperature for 1.5 hours, then take it out and cool it down to room temperature naturally , to obtain activated silica gel;

[0054] (II) Add molar concentration to activated silica gel and be 1mol / L NaOH aqueous solution (the mass ratio of described activated silica gel and NaOH aqueous solution is 1:0.5), and shake fully on the shaker until there is no agglomeration, obtain the described Alkaline silica gel.

preparation example 2

[0055] Preparation example 2: packing of chromatographic column

[0056] 1. Soak macroporous weakly acidic cation exchange resin CD180 resin in 95% ethanol for 20 hours.

[0057] 2. Take a 1-meter-long transparent glass tube with an inner diameter of 2 cm to fill the column, and plug the outlet at the lower end with absorbent cotton to prevent the contents from leaking out. Then, according to the volume of the glass tube, 20% basic alumina (commercially available, particle size 150 mesh), 15% neutral silica gel (commercially available, particle size 200 mesh), 10% basic silica gel (obtained by the above preparation example 1), 5% neutral alumina (commercially available, particle size is 200 mesh) and 30% macroporous weakly acidic cation exchange resin (CD180 resin processed through the above step 1).

[0058] 3. After filling, slowly add petroleum ether into the column to keep the liquid level above the basic alumina at the top of the column, and use a container at the lower ...

Embodiment 1

[0060] (1) The dried hooked stems of Uncaria macrophyllum are pulverized, and cross 100 mesh sieves to obtain the raw material powder; the raw material powder is added in the organic solvent shown in the following table 1 (the mass-to-volume ratio of the raw material powder to the organic solvent 1:15g / ml), then carbon dioxide (the volume mass ratio of the mixed organic solvent and carbon dioxide is 1:4ml / g), sealed and pressurized, so that the pressure is 2MPa, to form an expansion solvent system, and at 40 ℃ Under stirring and extracting for 2 hours, an extract was obtained;

[0061] (2) In a water bath at 40-50°C, purging the extract with nitrogen until it is nearly dry to obtain the initial concentrate;

[0062] (3) Adding mass percent concentration in the initial concentrate is 10% KH 2 PO 4 Aqueous solution and organic solvent (primary extraction concentrate and KH 2 PO 4 The mass volume ratio of the aqueous solution is 1:20g / ml, KH 2 PO 4 The volume ratio of the a...

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Abstract

The invention relates to a method for extracting a vasodilatation active compound rhynchophylline. The method comprises the following steps: (1), grinding the raw materials and sieving to obtain a raw material powder, adding the raw material powder into an organic solvent, importing carbon dioxide to form a swelling solvent system, and stirring and extracting to obtain an extracting solution; (2), concentrating the extracting solution to obtain a primarily extracted concentrate; (3), adding an inorganic matter solution and an organic solvent in the primarily extracted concentrate to carry out liquid-liquid extraction, layering to obtain an organic phase I, adding water into the organic phase I, stirring uniformly, layering to obtain an organic phase II and concentrating the organic phase II to obtain an extract; (4), dissolving the extract and filling to the top of a chromatography column, carrying out solvent elution, collecting the eluant, concentrating to obtain a crude product, and recrystallizing to obtain the rhynchophylline. According to the method, the obtained rhynchophylline is favorable in yield and purity, has a favorable application prospect and is potential in industrial production.

Description

technical field [0001] The invention relates to an extraction method of a plant active compound with pharmacological activity, and more specifically relates to an extraction method of a vasodilation active compound rhynchophylline, which belongs to the field of extraction of plant active components. Background technique [0002] Uncaria (Ramulus Uucariae cum uncis), formerly known as Uncaria, is an important traditional Chinese medicine in my country. It belongs to the Rubiaceae Uncaria genus. It is widely distributed in southern provinces of my country, such as Guizhou, Fujian, Jiangxi, Hunan, Yunnan, Guangdong, Guangxi, and Sichuan. and so on. There are many kinds, such as Uncaria, Uncaria, Uncaria, Uncaria, and Uncaria sessileus. [0003] The medicinal part of Uncaria is mainly the dry hooked stems of Uncaria plants. After research, it is found that its main components are Uncaria alkaloids and hedera aglycone, Uncaria aglycon A, Uncaria aglycon B, Uncaria aglycone Aglyc...

Claims

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Application Information

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IPC IPC(8): C07D471/20
CPCC07D471/20
Inventor 韩玮娜
Owner WEIFANG MEDICAL UNIV
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