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Preparation method of eucommia ulmoides geniposidic acid composite nanoparticles

A technology of composite nanoparticles and geniposidic acid, which is applied in the field of gliadin nanoparticles, can solve problems affecting bioavailability, achieve the effects of improving bioavailability, solving instability and insoluble, and improving solubility

Active Publication Date: 2021-06-22
INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Geniposidic acid (GPA) is an important natural active substance in bark, leaves and male flowers of Eucommia ulmoides, but due to the influence of solubility and stability, it is often limited in practical application, which greatly affects its Bioavailability

Method used

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  • Preparation method of eucommia ulmoides geniposidic acid composite nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1: Preparation method of Eucommia geniposidic acid composite nanoparticles

[0019] Accurately weigh 0.2g of zein protein Zein and dissolve it in 10mL of 80% (V / V) ethanol solution, add appropriate amount of geniposide respectively, and keep stirring for 2h in a closed environment at 25°C until the solution is clear. The mass ratios of zein to geniposidic acid in the obtained sample mixed solution were 100:1, 80:1, 60:1, 40:1 and 20:1. Place it at 4°C overnight to make it fully hydrated. Prepare a 1.0% pectin solution and inject the pectin solution into the zein Zein solution containing geniposidic acid under 1500rpm magnetic stirring, and continue to stir for 10 minutes to make it fully mixed so that the pectin content is 1.0%. Concentrate under reduced pressure at 50° C. and 60 r / min to remove ethanol, and then centrifuge at 3000 g for 5 min to remove a small amount of insoluble matter to obtain a zein Zein composite suspension. Adjust the pH of the solution...

Embodiment 2

[0023] Example 2: Evaluation of the bioavailability of Eucommia geniposidic acid composite nanoparticles

[0024] A certain amount of DPPH sample was weighed and dissolved in ethanol to prepare a concentration of 0.05 mg / mL. Prepare the sample solution to be tested at 1.0mg / mL, add 2.0mL of DPPH test solution to a 10mL colorimetric tube, add the sample solution in sequence to 100μL, then add 95% ethanol to make it to 6.0mL, mix well, and react in the dark for 30min. The absorbance was detected at 517nm. The absorbance of the experimental group is recorded as A 1 , the absorbance of the blank group is recorded as A 0 , according to the formula to calculate the scavenging rate of different samples to DPPH free radicals.

[0025]

[0026] Prepare 4.9mM potassium persulfate solution and 7.0mM ABTS solution, and mix them in equal volumes. React at room temperature in the dark for 12-16 hours to form an ABTS free radical stock solution. The ABTS stock solution was diluted wi...

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Abstract

The invention discloses a preparation method of eucommia ulmoides geniposidic acid composite nanoparticles, zein loaded geniposidic acid composite nanoparticles are prepared by using the unique self-assembly characteristic of zein, taking polysaccharide as a stabilizer and utilizing an anti-solvent coprecipitation method; the operation is simple, the condition is mild, the cost is lower, and the method is green and environment-friendly. According to the zein-loaded geniposidic acid composite nano-particles, geniposidic acid is embedded in the protein nano-particles, so that the stability of the protein nano-particles is effectively improved, the solubility of the protein nano-particles is improved, the prepared zein-loaded geniposidic acid composite nano-particles are uniform in particle size, high in embedding rate and relatively good in stability, the bioavailability of the natural active ingredient geniposidic acid is improved, and the bioavailability of the zein-loaded geniposidic acid composite nano-particles is improved. The problems that geniposidic acid is unstable and difficult to dissolve are solved.

Description

Technical field: [0001] The invention relates to the technical field of gliadin nanoparticles, in particular to a preparation method of eucommia geniposidic acid composite nanoparticles. Background technique: [0002] Geniposidic acid (GPA) is an important natural active substance in bark, leaves and male flowers of Eucommia ulmoides. Bioavailability. Embedding natural active ingredients into nanoparticles is a common method to solve this problem. The method can protect the unstable active ingredient from being degraded in a complex environment, uniformly disperse the water-insoluble active ingredient in the water phase, and improve the bioavailability of the active ingredient. Most of the materials currently used to embed natural active ingredients are derived from natural, safe and readily available biological materials. Natural biomaterials have superior biocompatibility and nutritional value. Protein-based nanoparticles can significantly increase the bioavailability ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/42A61K47/36A61K31/7048A61P39/06
CPCA61K9/146A61K31/7048A61P39/06Y02A50/30
Inventor 王志宏彭密军杨秋玲黄韬
Owner INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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