Application of compounds with collagen synthesis promoting activity in anti-aging cosmetics
A technology for collagen synthesis and cosmetics, applied in cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of unsatisfactory anti-aging effects, delay and inhibit skin aging, simple extraction method and high purity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1 Preparation method of aucubin and genipocurin
[0041] Steps include:
[0042] (1) Take dried eucommia male flowers, add 90v% ethanol solution, the mass ratio of solid to liquid is 1:14, ultrasonically extract 3 times, each time for 30min, combine the filtrates, and remove ethanol;
[0043] (2) Adsorb with a macroporous adsorption resin after removing ethanol, and the macroporous adsorption resin is preferably H103; wash with water to remove impurities such as carbohydrates and proteins, then elute with an ethanol solution with a volume concentration of 40v%, collect and wash Deliquified, concentrated and freeze-dried;
[0044] (3) Take the lyophilized product, dissolve it with eluent, load the sample on the column with a sample volume of 25 mg / mL, and use chloroform-methanol (V:V=5:1) as eluent at a flow rate of 2 BV / h. Deliquification, concentration under reduced pressure to obtain crude product;
[0045] (4) Make the crude product of step (3) into 100mg / ...
Embodiment 2
[0046] Example 2 Aucubin or geniposide promotes the proliferation of skin fibroblasts in vitro
[0047] ESF-1 cells in the logarithmic growth phase were taken, the old culture medium was discarded, trypsin was added, and after digestion (observation of cell morphology under a microscope), DMEM culture medium containing 10wt% fetal bovine serum was added (DMEM dry powder was purchased from Gibco Company, For the preparation method, refer to its instruction manual), blow and beat repeatedly with an elbow pipette several times to make the cells detached from the bottle wall, evenly suspend in the culture medium, put them in a centrifuge tube, centrifuge at 1000 r / min for 5 min, discard the supernatant; add an appropriate amount of DMEM culture medium containing 10wt% fetal bovine serum was pipetted several times to suspend the cells in the culture medium. Place the cell solution in a sterile dish and pipette evenly, count on the cell counting board to adjust the cell concentratio...
Embodiment 3
[0051] Example 3 Determination of the content of collagen I in fibroblasts by enzyme-linked immunosorbent assay (ELISA)
[0052] The fibroblasts were digested by trypsin and transferred to a 96-well culture plate. The edge wells were filled with sterile PBS (0.01M), and moved into the incubator to continue culturing. 10 μL / well (concentrations: 30, 20, 10, 5, 0.5 μM), 6 replicate wells in each group, the same volume of PBS (0.01M) was added to the control group, and 48 hours after the addition of the drug, the test was carried out according to the kit instructions. type collagen content. The sample without adding active ingredients was used as the control group, and its collagen synthesis was 100%. The experimental results are shown in Table 2 below.
[0053] Table 2 The promoting effect of compounds on collagen synthesis in fibroblasts (%)
[0054]
[0055] As shown in Table 2, compared with the control group that does not contain the active ingredient of the present inv...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap