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Application of compounds with collagen synthesis promoting activity in anti-aging cosmetics

A technology for collagen synthesis and cosmetics, applied in cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of unsatisfactory anti-aging effects, delay and inhibit skin aging, simple extraction method and high purity

Active Publication Date: 2017-08-25
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-aging efficacy cosmetics on the market have unsatisfactory anti-aging effects and need to be further improved.

Method used

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  • Application of compounds with collagen synthesis promoting activity in anti-aging cosmetics
  • Application of compounds with collagen synthesis promoting activity in anti-aging cosmetics
  • Application of compounds with collagen synthesis promoting activity in anti-aging cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation method of aucubin and genipocurin

[0041] Steps include:

[0042] (1) Take dried eucommia male flowers, add 90v% ethanol solution, the mass ratio of solid to liquid is 1:14, ultrasonically extract 3 times, each time for 30min, combine the filtrates, and remove ethanol;

[0043] (2) Adsorb with a macroporous adsorption resin after removing ethanol, and the macroporous adsorption resin is preferably H103; wash with water to remove impurities such as carbohydrates and proteins, then elute with an ethanol solution with a volume concentration of 40v%, collect and wash Deliquified, concentrated and freeze-dried;

[0044] (3) Take the lyophilized product, dissolve it with eluent, load the sample on the column with a sample volume of 25 mg / mL, and use chloroform-methanol (V:V=5:1) as eluent at a flow rate of 2 BV / h. Deliquification, concentration under reduced pressure to obtain crude product;

[0045] (4) Make the crude product of step (3) into 100mg / ...

Embodiment 2

[0046] Example 2 Aucubin or geniposide promotes the proliferation of skin fibroblasts in vitro

[0047] ESF-1 cells in the logarithmic growth phase were taken, the old culture medium was discarded, trypsin was added, and after digestion (observation of cell morphology under a microscope), DMEM culture medium containing 10wt% fetal bovine serum was added (DMEM dry powder was purchased from Gibco Company, For the preparation method, refer to its instruction manual), blow and beat repeatedly with an elbow pipette several times to make the cells detached from the bottle wall, evenly suspend in the culture medium, put them in a centrifuge tube, centrifuge at 1000 r / min for 5 min, discard the supernatant; add an appropriate amount of DMEM culture medium containing 10wt% fetal bovine serum was pipetted several times to suspend the cells in the culture medium. Place the cell solution in a sterile dish and pipette evenly, count on the cell counting board to adjust the cell concentratio...

Embodiment 3

[0051] Example 3 Determination of the content of collagen I in fibroblasts by enzyme-linked immunosorbent assay (ELISA)

[0052] The fibroblasts were digested by trypsin and transferred to a 96-well culture plate. The edge wells were filled with sterile PBS (0.01M), and moved into the incubator to continue culturing. 10 μL / well (concentrations: 30, 20, 10, 5, 0.5 μM), 6 replicate wells in each group, the same volume of PBS (0.01M) was added to the control group, and 48 hours after the addition of the drug, the test was carried out according to the kit instructions. type collagen content. The sample without adding active ingredients was used as the control group, and its collagen synthesis was 100%. The experimental results are shown in Table 2 below.

[0053] Table 2 The promoting effect of compounds on collagen synthesis in fibroblasts (%)

[0054]

[0055] As shown in Table 2, compared with the control group that does not contain the active ingredient of the present inv...

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Abstract

The application of a compound with the activity of promoting collagen synthesis in anti-aging cosmetics belongs to the technical field of cosmetics, and the compound is aucubin or genipinic acid. The extraction method of the two compounds of aucubin and geniposidic acid of the present invention is simple, the purity of the extract is high, and these two compounds can be applied to cosmetics to make liquid, emulsion or cream , to promote the synthesis of skin collagen, delay and inhibit the effect of skin aging.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to the application of a compound with the activity of promoting collagen synthesis in anti-aging cosmetics. Background technique [0002] The skin is the largest organ covering the surface of the human body. Due to direct contact with the external environment and stimulation by various stress factors such as oxidation, dryness and ultraviolet rays, the skin is very vulnerable to damage. At the same time, due to the influence of internal factors such as age, diet and living habits, skin function declines and genetic abnormalities lead to aging of the skin. Because the skin is the outermost part of the human body, it clearly expresses the beauty of the human body. Therefore, anti-aging cosmetics have become one of the products that the majority of female consumers urgently need. [0003] The determinant of skin aging is the destruction of collagen in the dermis by the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/60A61Q19/08
Inventor 丁艳霞李钦赵芬琴杜丽敏徐艳茹胡倩影田己鑫
Owner HENAN UNIVERSITY
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