66results about How to "Increase spleen index" patented technology

The invention relates to a composition for strengthening an immune regulation function in a human body and applications thereof. The composition comprises effective components of panax japonicus saponin and panax japonicus polysaccharide or the effective components and an acceptable carrier on a health medicament, health food, health tea, health wine or a beverage. The composition has the function of improving the immune function of an immunocompromised mouse. In an animal model with a weak immune system caused by cyclophosphamide (Cy), the composition can enhance the spleen index of the immunocompromised mouse, enhance the proportions of CD<4+> to CD<8+> and B lymphocytes to NK cells, and increase the content of cell factors IL-2 and IFN-gamma. The composition enhances the medicament effect and the medical value of panax japonicus; therefore, the planting of medicinal materials and the development of medicaments can be promoted. The composition has better social and economic benefits.

The invention discloses a use of butenolide I for preparing drugs for controlling immunological liver injury. With the present invention, the butenolide I can reduce levels of TNF-alpha and NO, wherein the TNF-alpha and the NO play important roles in a pathogenic process of the immunological liver injury; the butenolide I can reduce elevated levels of the NO and the iNOS in liver homogenates of immunological liver injury mice so as to decrease the liver injury induced by the NO; the butenolide I can inhibit a overexpression of the TNF-alpha so as to decrease the direct liver injury or the indirect liver injury induced by the TNF-alpha. In addition, the use of the butenolide I as the drug for controlling the immunological liver injury has advantages of convenient preparation, extensive sources, low price and high safety, and has good development and application prospect.

The invention relates to traditional Chinese medicine compound polysaccharide with a function of synergistically enhancing humoral and cellular immunity. The traditional Chinese medicine compound polysaccharide comprises the following components in parts by mass: 25 parts of lentinan, 50 parts of astragalus polysaccharide and 125 parts of odonopsis pilosula polysaccharide. The optimal dosage of the traditional Chinese medicine compound polysaccharide for oral administration of a mouse is 1 g per kilogram of body weight per day, and the mouse is continuously dosed for 7-10 days; the optimal dosage of the traditional Chinese medicine compound polysaccharide of a pig is 0.111 g per kilogram of body weight per day, and the pig is continuously dosed for 14-21 days. The three polysaccharides are scientifically and reasonably matched and combined to generate complementary and synergetic enhancement functions, the formed polysaccharide composition can comprehensively recover and enhance the immunity of organism, thereby having an obvious immunity enhancement effect, and the components of the traditional Chinese medicine compound polysaccharide are common raw medicinal materials, thereby being good in safety and free of toxic side effect.

The invention discloses an environmentally-friendly compound Chinese herbal medicinal feed additive for enhancing the immunities of broilers, and an application thereof, and belongs to the technical field of animal feed additives. The bulk drugs of the additive comprise Radix Astragali, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Poria cocos, hyacinth bean, medicated leaven, hawthorn fruit and malt. The additive is prepared through drying the bulk drugs at 50-60DEG C, crushing, sieving, and mixing 29-31g of Radix Astragali, 29-31g of Radix Codonopsis, 29-31g of Rhizoma Atractylodis Macrocephalae, 29-31g of Poria cocos, 29-31g of hyacinth bean, 14-16g of medicated leaven, 14-16g of hawthorn fruit and 14-16g of malt The prepared additive is added to a mixed broiler feed according to a mass percentage of 0.5-1.5% to obtain a mixture for use in order to substantially reduce the feed conversion ratio and increase the spleen index, the thymus index and the index of bursa of Fabricius.

The invention belongs to the technical field of feed compositions and relates to growing-period feed capable of promoting the development of digestive organs of laying hens and a preparation method. The growing-period feed is mainly prepared from the following raw materials in parts by weight: 45 to 60 parts of cereal, 8 to 15 parts of soybean meal, 1 to 8 parts of miscellaneous meal, 6 to 15 parts of bran, 3 to 8 parts of pomace, 2 parts of evening primrose meal, 0.8 to 1.5 parts of mountain flour, 0.8 to 1.5 parts of calcium hydrogen phosphate, 0.35 to 0.43 part of amino acid, 0.5 to 15 parts of vegetable oil, 1 part of compound premix, 0.05 to 0.2 part of prebiotics, 0.01 to 0.05 part of zinc methionine, 0.05 to 0.2 part of hydrolyzed tannic acid, 0.05 to 0.08 part of a compound enzyme preparation, 0.02 to 0.1 part of a mycotoxin adsorbent, 0.04 to 0.2 part of a mycotoxin degradation enzyme, 0.1 to 0.5 part of yeast hydrolysate and 1 to 5 parts of dietary fiber. By adopting the growing-period feed, indexes of all the digestive organs at a growing period of the laying hens are improved to different degrees.

The present invention relates the application of nano copper powder in preparation of immunity-enhancing medicine. It adopts the commercial nano copper powder whose grain size is 0.1-1000 nm as raw material, the weight ratio of raw material nano copper powder required for preparing medicine and preparation is 0.002-0.04:1. Said nano copper powder has the enhancement for specific immunity and non-specific immunity of body, and said medicine can be used for curing various diseases due to infective microbe and its toxin, tumor, dead cell or mutant cell.

The invention discloses an anti-radiation traditional Chinese medicine composition which comprises the following components in parts by weight: 160-200 parts of membranous milkvetch root, 110-130 parts of scurfy pea, 80-100 parts of Chinese angelica, 160-200 parts of epimedium, 135-165 parts of madder root, 110-130 parts of glossy privet fruit, 110-130 parts of white paeony root, 110-130 parts ofradix rehmanniae preparata, 80-100 parts of Szechuan lovage rhizome, 215-265 parts of Chinese yam and 35-45 parts of prepared liquoric root. The traditional Chinese medicine composition can increase thymus index and spleen index of a 60Co gamma-radiated mouse and protect immune organs; improve the activity of superoxide dismutase in blood serum and the liver of the 60Co gamma-radiated mouse, reduce the content of malonaldehyde and improve the anti-oxidation capability of an organism; increase peripheral white blood cells of the 60Co gamma-radiated mouse and improve the hematogenesis function;and reduce the micronuclear rate of marrow cells of the 60Co gamma-radiated mouse and inhibit the action of causing chromosomal aberration of 60Co gamma rays. In addition, the traditional Chinese medicine composition is low in toxicity and side effects, and medicinal materials in the formula are low in price and easy to obtain, thereby having great development and application prospects in the aspects of preventing and treating radiation injuries.

The invention relates to health-care and antihypertensive hepherdspuse herb biscuits. The invention further relates to a preparation method of the health-care and antihypertensive hepherdspuse herb biscuits. The health-care and antihypertensive hepherdspuse herb biscuits are prepared from, by mass, 40-60 parts of hepherdspuse herbs, 35-50 parts of wheat flour, 5-10 parts of auricularia auricula, 3-9 parts of lentinula edodes, 5-11 parts of pumpkins, 4-12 parts of hawthorns, 4-10 parts of herba taxilli, 3-9 parts of puerarin, 4-9 parts of raw sophora flowers, 3-9 parts of radix scutellariae, 3-8 parts of lotus seeds, 3-8 parts of parsley, 2-9 parts of magnolia denudata, 3-9 part of stigma maydis, 3-9 parts of polygonum multiflorum and 10-20 parts of sunflower seed oil. The health-care and antihypertensive hepherdspuse herb biscuits are rich in nutrient, suitable for the old and the young and capable of achieving the health-care efficacy after being eaten for a long time.

The present invention relates the application of nano copper powder in preparation of immunity-enhancing medicine. It adopts the commercial nano copper powder whose grain size is 0.1-1000 nm as raw material, the weight ratio of raw material nano copper powder required for preparing medicine and preparation is 0.002-0.04:1. Said nano copper powder has the enhancement for specific immunity and non-specific immunity of body, and said medicine can be used for curing various diseases due to infective microbe and its toxin, tumor, dead cell or mutant cell.

The present invention discloses a fermented feed containing humic acid chelated trace elements. Bacterium strains are inoculated into a fermentation medium containing the humic acid chelated trace elements and the mixture is fermented to obtain the fermented feed containing the humic acid chelated trace elements. The fermentation medium is composed of fermentation materials and water. The raw materials of the fermentation materials consist of 10-20 parts of soybean meal, 20-45 parts of bran, 5-10 parts of humic acids, 40-50 parts of stone powder, 5-10 parts of chloranthus fortunei, 5-10 parts of chloranthus japonicus, 4-8 parts of apocynum venetum leaves, 4-8 parts of conyza blinii, 4-8 parts of folium microcotis and 0.7-1.4 parts of the humic acid chelated trace elements. The present invention also discloses a preparation method of the fermented feed. The fermented feed is prepared by the chelation of the humic acids and trace elements, helps alleviate the problems of resource wastes and environmental pollutions caused by the additions of the inorganic trace elements, and solves the problems that the amino acid trace element chelate is high in production costs and not conducive to wide uses.

The invention discloses a traditional Chinese medical composition for prevention and control of avian influenza and a preparation method thereof. The traditional Chinese medical composition is prepared with astragali, couch grass roots and licorice according to a ratio, and can be applied to improve the immunity. Moreover, the traditional Chinese medical composition has significant effects on prevention and control of the avian influenza and can be widely applied in animals.

The invention discloses an active polypeptide with an antagonistic chemotactic factor receptor CCR5, which has an amino acid sequence in SEQ ID NO:1 or an amino acid sequence obtained by carrying out deletion, addition, insertion and substitution of one or more amino acid residues on the amino acid sequence in SEQ ID NO:1. The invention also discloses a preparation method of the polypeptide, which comprises the following steps of: searching related protein sequences in a database; carrying out multiple alignment comparative analysis to screen out a highly conservative region for combination of a vMIP-II receptor; eliminating interference of unrelated residues through analysis simulation means; determining a target peptide sequence; synthetizing the active polypeptide by a solid-phase synthesis method; and finally testing the bioactivity of the polypeptide. The polypeptide can be used as a CCR5 receptor antagonist for the development of an anti-inflammatory drug, can be used as a lead drug for treating AIDS (Acquired Immune Deficiency Syndrome) and can also be used as a medicament with the immune regulation effect or a medicament for improving the functions of an immune system of an organism.

The invention belongs to the technical field of food, and discloses a black garlic extract and application thereof in preventing and curing breast cancer. It is proved by the cell test that the black garlic extract inhibits growth of breast cancer MD231 cells and T47D cells in a concentration-dependent and time-dependent manner, so that the adhesiveness among cancer cells is enhanced, and the vigor of the cancer cells is reduced; besides, it is proved by the animal test that the black garlic extract can inhibit growth of nude mouse MCF-7 transplanted tumor remarkably, the gross tumor volume is reduced, the thymus index and the spleen index are increased, tumor immunosuppression is avoided, and the black garlic extract has the immunological enhancement effect. Therefore, the black garlic extract can be used for preventing and curing breast cancer, is rich in source and pure-natural, and has important development and application prospects.

The invention relates to a preparation method of RGD cyclic peptide R8 peptide modified ergosterol and gefitinib combined compound liposome freeze-dried powder. The preparation method comprises the following steps: adding a freeze-drying protective agent into a pre-prepared RGD / R8-ERG / GEF-LIP liposome suspension in an external addition manner; and finally, preparing the freeze-dried powder of thecompound liposome by adopting a freeze-drying method. The RGD / R8-ERG / GEF-LIP lipidosome suspension is prepared by adopting the following method: firstly, preparing ERG / GEF-LIP, and then, preparing theRGD / R8-ERG / GEF-LIP lipidosome suspension by adopting a post-insertion method. According to the invention, an RGD / R8-ERG / GEF-LIP active drug-loading liposome drug delivery system is successfully constructed; ERG / GEF-LIP is used for investigating a freeze-drying process and a prescription, and after an optimal prescription process is screened out, the optimal prescription process is applied to RGD / R8-ERG / GEF-LIP for verification. An in-vitro test result of RGD / R8-ERG / GEF-LIP freeze-dried powder proves that the RGD / R8-ERG / GEF-LIP freeze-dried powder has a relatively strong tumor cell proliferation inhibition effect, the fluorescence uptake intensity and the good cell apoptosis rate.

The invention provides acylated glycoside compounds as well as a production method and application thereof. The acylated glycoside compounds are characterized by comprising the following structural general formula: R1 is H, OH or OAc and R2 is toluenesulfonyl group and salicyl. The acylated glycoside compounds disclosed by the invention have an immune regulation function and can be used for preparing medicaments for treating rheumatoid arthritis, medicaments for treating autoimmune diseases such as systemic lupus erythematosus, eczema and parapsoriasis guttata as well as medicaments for treating various immunological diseases such as allergic reaction.

The invention provides a medicated diet composition for enhancing immunity. The medicated diet composition comprises, by weight, 60-75 parts of selenium-rich grifola frondosa, 35-45 parts of selenium-rich agaricus blazei murill, 30-40 parts of selenium-rich radix astragali, 2-10 parts of gypenoside and 2-10 parts of glutathione, wherein the selenium contents of the selenium-rich grifola frondosa,the selenium-rich agaricus blazei murill and the selenium-rich radix astragali are greater than 400 ppm. The invention further provides a preparation method of the medicated diet composition. The method is based on water extraction and repeated low-temperature extraction. The medicated diet composition has the advantages that components are reasonably compounded, the formulation is ingenious, andthe composition has the effect of regulating the balance of qi, blood, yin and yang of the human body and enhancing the body immunity. The preparation method has the advantages that through repeated low-temperature extraction, active ingredients can be extracted as many as possible on the premise of ensuring the activity of the active ingredients.

The invention discloses a Zhuang medicine composition assisted in resisting lung cancer. The Zhuang medicine composition is characterized by being prepared from the following Zhuang medicinal materials: Nyarinngoux (Chinese name: baihuasheshecao), Gaeubwnhgauh (Chinese name: baiying), Byaekloekhauj (Chinese name: longkui), Buenqcilienz (Chinese name: banzhilian), Ginghgun (Chinese name: guangxi eshu), Fouxndoengz (Chinese name: shishangbai), Swnjgyaeujhenj (Chinese name: huanghuadaoshuilian), Raggongox (Chinese name: lugen), GO gyauhgujlanz (Chinese name: jiaogulan) and Lozhangoj (Chinese name: luohanguo). The Zhuang medicine composition is combined with chemical drugs for curing lung cancer, can reduce the toxic and side effects of inappetence caused by the chemical drugs, reduce the damage of the chemical drugs to immune organs, increase white blood cell count, blood platelet count, red blood cell count and hemoglobin count to reduce the inhibiting effect on myelosuppression, reduce the content of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase to alleviate the damage to liver functions and increase the tumor suppression function of the chemical drugs. Namely, the Zhuang medicine composition is combined with the chemical drugs for curing the lung cancer and has the effects of toxicity reducing and efficacy enhancing.

The invention discloses a Chinese medicine composite capable of improving immunity. The composite comprises the following components: 30-60% of barbary wolfberry fruit, 10-50% of white atractylodes rhizome, 10-50% of Chinese yam, 10-40% of liquorice and 10-40% of medicated leaven. The composite can be prepared into medicines in the forms of powder, liquid, paste, particles or capsules or a pharmaceutically acceptable physical form. According to the Chinese medicine composite disclosed by the invention, the survive rate can reach 70%, the immunity is effectively improved, and specific to the immune organ indexes, the spleen index is improved by 23.2 percent, the thymus index is improved by 32.4%, and the index of bursa of Fabricius is improved by 41.2%; and especially, in terms of the contents of serum globulins, the content of IgA (immunoglobulin A) is improved by 20.7%, and the content of IgY (immunoglobulin Y) is improved by 23.4%, so that the composite disclosed by the invention can obviously promote specific immunity.

The invention relates to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for treating a porcine reproductive and respiratory syndrome andan application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises saffron crocus, fructus amomi, folium perillae, radix rehmanniae recen, gastrodia elata, ganoderma lucidum polysaccharide, folium artemisiae argyi, a glossy privet fruit, acanthopanax and dandelion. According to the composition, different traditional Chinese medicine ingredientsare matched for use; the traditional Chinese medicine composition is based on enhancement and repairing of a porcine immune system, and has functions of enhancing phagocytosis of mononuclear macrophages, activating lymphocytes, promoting immune response of a body, and enhancing and regulating humoral immunity; based on a combined effect of traditional Chinese medicine active ingredients, the composition directly eliminates reproductive and respiratory syndrome viruses, controls development of viremia, can effectively rise an antibody level of the porcine reproductive at the same time, and respiratory syndrome and enhances porcine immunologic functions and disease resistance.

The invention discloses an application of codonopsis pilosula, rhizoma atractylodis macrocephalae, poria and liquorice powder in preparing medicament for improving immunity of rabbits. According to the application, the codonopsis pilosula, rhizoma atractylodis macrocephalae, poria and liquorice powder accounting for 1-5 percent of the weight of basic ration is added into the basic ration, the basic ration is fed to rabbits, and the rabbit sacculus rotundus index, thymus index, spleen index or dendritic index of a rabbit can be increased, the immunity of the rabbit can be improved, the expression quantity of an antifungal protein gene can be increased, and theoretical direction is provided to the codonopsis pilosula, rhizoma atractylodis macrocephalae, poria and liquorice powder in improving rabbit immunity.

The invention belongs to the technical field of traditional Chinese medicines, and in particular provides a medicament composition for treating cognitive dysfunction. The medicament composition is prepared from the following components in parts by weight by virtue of combination: 10-100 parts of manyinflorescaned sweetvetch root, 8-80 parts of baikal skullcap root, 5-60 parts of root of kirilow rhodiola, 5-60 parts of rhizoma acori graminei and 3-50 parts of earth worm. The invention also provides a preparation method and specific application of the medicament composition. Experiments prove that the medicament composition provided by the invention can significantly improve the learning and memorizing abilities of a vascular dementia rat model and reduce activity of MDA and MAO in hippocampus tissues and serums, and can improve mouse pia mater local microcirculation disorders caused by norepinephrine in different degrees to ensure that micro arteries are expanded, and the blood stream is accelerated; and experimental results show that the medicament composition provided by the invention has an obvious treatment effect on vascular dementia.

The invention relates to a traditional Chinese medicine composition with qi-tonifying and blood-nourishing effects and a preparation method thereof. The traditional Chinese medicine composition is prepared by processing radix codonopsis, radix rehmanniae preparata, radix angelicae sinensis, poria cocos, radix paeoniae alba, rhizoma atractylodis macrocephalae stir-fried with bran, rhizoma chuanxiong and licorice roots. The traditional Chinese medicine composition has the effects of tonifying qi and nourishing the blood and is used for qi-blood deficiency, sallow complexion, inappetence, limb weakness, menometrorrhagia and the like. The traditional Chinese medicine composition does not contain ethyl alcohol and is small in irritation to gastrointestinal tracts and suitable for females not good at drinking, patients who have gastrointestinal ulcers and the like and need to be prohibited from drinking wine and people who have allergy to alcohol for drinking.

The invention discloses an anti-radiation traditional Chinese medicine composition which comprises the following components in parts by weight: 160-200 parts of membranous milkvetch root, 110-130 parts of scurfy pea, 80-100 parts of Chinese angelica, 160-200 parts of epimedium, 135-165 parts of madder root, 110-130 parts of glossy privet fruit, 110-130 parts of white paeony root, 110-130 parts of radix rehmanniae preparata, 80-100 parts of Szechuan lovage rhizome, 215-265 parts of Chinese yam and 35-45 parts of prepared liquoric root. The traditional Chinese medicine composition can increase thymus index and spleen index of a 60Co gamma-radiated mouse and protect immune organs; improve the activity of superoxide dismutase in blood serum and the liver of the 60Co gamma-radiated mouse, reduce the content of malonaldehyde and improve the anti-oxidation capability of an organism; increase peripheral white blood cells of the 60Co gamma-radiated mouse and improve the hematogenesis function; and reduce the micronuclear rate of marrow cells of the 60Co gamma-radiated mouse and inhibit the action of causing chromosomal aberration of 60Co gamma rays. In addition, the traditional Chinese medicine composition is low in toxicity and side effects, and medicinal materials in the formula are low in price and easy to obtain, thereby having great development and application prospects in the aspects of preventing and treating radiation injuries.

The invention provides a novel application of a selaginella extract for resisting the irradiatino-damage, and provides the application of selaginella total-extracts and a selaginella water-fraction for resisting the irradiatino-damage. An experiment shows that the selaginella extract can inhibit the damage on organisms caused by radiation, and prolong the survival time of the organisms; the selaginella water-fraction can obviously inhibit the thymocyte and splenic cell apoptosis of the organisms caused by the radiation. The novel application has the advantages that the inordinate mitotic cycle is regulated, the body immune function is enhanced, and the radioprotection effect on the hemopoietic tissue is realized obviously.

The invention provides a composition with an immunity enhancement effect and belongs to the technical field of immunoregulation drugs. The composition comprises garlic lectin, alliin and garlic polysaccharide in a mass ratio being (0.4-0.6):(0.8-1.2):(17-25). The composition can increase a spleen index and a thymus index of immunosuppression mice, increase total white blood cells, a clearance index K and a phagocytic index alpha and ensure content levels of IL-2, TNF-alpha and IFN-gamma in serum to approach to normal levels and has the significant immunity enhancement effect.

The invention provides a novel application of a selaginella extract for resisting the irradiatino-damage, and provides the application of selaginella total-extracts and a selaginella water-fraction for resisting the irradiatino-damage. An experiment shows that the selaginella extract can inhibit the damage on organisms caused by radiation, and prolong the survival time of the organisms; the selaginella water-fraction can obviously inhibit the thymocyte and splenic cell apoptosis of the organisms caused by the radiation. The novel application has the advantages that the inordinate mitotic cycle is regulated, the body immune function is enhanced, and the radioprotection effect on the hemopoietic tissue is realized obviously.

The invention discloses a ginseng-mai polysaccharide preparation and a preparation method thereof. The preparation method comprises the following steps: respectively adding red ginseng and Radix Ophiopogon japonicus into ethanol for impregnation, then heating and refluxing for extraction, recovering the extraction residue, drying in a desiccator, and preparing Red ginseng medicinal residue and Ophiopogon japonicus medicinal residue; mixing red ginseng medicinal residue and Ophiopogon japonicus medicinal residue, adding distilled water for extraction, and extracting twice; combining the extracts, concentrating and filtering them to prepare a concentrate; Add absolute ethanol to the mixture, let it stand overnight, centrifuge to collect the precipitate, wash it with absolute ethanol and acetone in turn, and then centrifuge; place the precipitate in a freeze-drying equipment, vacuum freeze-dry it, and grind it to obtain Shenmai polysaccharide powder . The preparation process of the polysaccharide preparation is simple, the cost is low, the extraction residue of Shenmai injection is reused, and the polysaccharide preparation has a good immune enhancing effect.

The invention provides a traditional Chinese medicine fungicide as well as a preparation method and application thereof. The fungicide comprises probiotic liquid, a traditional Chinese medicine extract, a vitamin additive and a trace element additive. According to the traditional Chinese medicine fungicide, the average daily gain and the average daily feed intake of Putian black ducks can be remarkably increased, and the feed conversion ratio is remarkably reduced. The average laying number of the Putian black ducks and the average quality of single eggs are obviously increased. The thymus index, bursa of Fabricius index, spleen index and serum IgG content of the Putian black ducks are remarkably increased, the hatching rate of the Putian black duck eggs is remarkably increased, and the mercury, lead and cadmium content of leg muscles of the Putian black ducks is remarkably reduced. The average daily gain and the average daily feed intake of pigs can be remarkably increased, and the feed conversion ratio is remarkably reduced; and the slaughter rate and the net meat rate of the pigs are remarkably increased, the carcass cooling loss rate is remarkably reduced, and the mercury, lead and cadmium contents of pig hind leg muscles are reduced.

The invention relates to a traditional Chinese medicine compound polysaccharide which can synergistically enhance humoral and cellular immunity, comprising lentinan, 25 parts by mass; astragalus polysaccharide, 50 parts; and codonopsis polysaccharide, 125 parts. The optimal dose of the traditional Chinese medicine compound polysaccharide for oral administration to mice is 1g per kilogram of body weight per day for 7-10 consecutive days; the optimal dosage for pigs is 0.111g per kilogram of body weight per day for 14-21 consecutive days . In this invention, the scientific and reasonable combination of three-flavored polysaccharides can produce complementary and coordinated enhancement effects. The formed polysaccharide composition can fully restore and enhance the immune function of the body, and has obvious immune-enhancing effects, and they are all commonly used raw materials. Good safety, no toxic side effects.

The invention discloses a traditional Chinese medical composition for prevention and treatment of A type clostridiosis welchii, which consists of the following components by weight: 10-80 parts of mahonia fortunei, 5-50 parts of pulsatilla chinensis, 3-50 parts of yam, 3-30 parts of semen plantaginis, 3-30 parts of sanguisorba officinalis, 3-30 parts of rheum officinale and 5-45 parts of acalypha australis. The invention also discloses an extract prepared with the composition, a preparation method of the extract, a preparation prepared with the extract and application of the preparation. The composition, the extract and the preparation have broad values in medical application for prevention and treatment of the A type clostridiosis welchii.