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Preparation method of ergosterol and gefitinib combined compound liposome freeze-dried powder, liposome and application thereof

A compound liposome and gefitinib technology, which is applied in liposome delivery, medical preparations of non-active ingredients, freeze-dried delivery, etc., can solve problems such as multidrug resistance and achieve strong tumor cell proliferation inhibition The effect of good effect and fluorescence uptake intensity

Active Publication Date: 2019-12-31
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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Problems solved by technology

[0003] At present, the clinical first-line chemotherapy drugs (platinum, doxorubicin, paclitaxel, etc.) will have multidrug resistance during chemotherapy, which will eventually lead to further progression of the disease

Method used

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  • Preparation method of ergosterol and gefitinib combined compound liposome freeze-dried powder, liposome and application thereof
  • Preparation method of ergosterol and gefitinib combined compound liposome freeze-dried powder, liposome and application thereof
  • Preparation method of ergosterol and gefitinib combined compound liposome freeze-dried powder, liposome and application thereof

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Embodiment Construction

[0051] The present invention will be further explained below in conjunction with the accompanying drawings.

[0052] This specific embodiment is only an explanation of the present invention, but not a limitation of the present invention. Any changes made by those skilled in the art after reading the description of the present invention will be protected by the patent law as long as they are within the scope of the claims.

[0053] Preparation and quality evaluation of cyclic peptide / R8 peptide modified ERG combined with GEF compound liposome freeze-dried powder

[0054] In this part, the DSPE-PEG is inserted by post-insertion method 3400 -c (RGDfk) and DSPE-PEG 1000 -R8 is embedded in the lipid membrane of ERG / GEF-LIP, and the RGD cyclic peptide / R8 peptide modified ERG / GEF-LIP (RGD / R8-ERG / GEF-LIP) is prepared. Considering the stability of the liposome itself, Prepare it in the form of freeze-dried powder, conduct preliminary investigations on its morphology, particle size d...

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Abstract

The invention relates to a preparation method of RGD cyclic peptide R8 peptide modified ergosterol and gefitinib combined compound liposome freeze-dried powder. The preparation method comprises the following steps: adding a freeze-drying protective agent into a pre-prepared RGD / R8-ERG / GEF-LIP liposome suspension in an external addition manner; and finally, preparing the freeze-dried powder of thecompound liposome by adopting a freeze-drying method. The RGD / R8-ERG / GEF-LIP lipidosome suspension is prepared by adopting the following method: firstly, preparing ERG / GEF-LIP, and then, preparing theRGD / R8-ERG / GEF-LIP lipidosome suspension by adopting a post-insertion method. According to the invention, an RGD / R8-ERG / GEF-LIP active drug-loading liposome drug delivery system is successfully constructed; ERG / GEF-LIP is used for investigating a freeze-drying process and a prescription, and after an optimal prescription process is screened out, the optimal prescription process is applied to RGD / R8-ERG / GEF-LIP for verification. An in-vitro test result of RGD / R8-ERG / GEF-LIP freeze-dried powder proves that the RGD / R8-ERG / GEF-LIP freeze-dried powder has a relatively strong tumor cell proliferation inhibition effect, the fluorescence uptake intensity and the good cell apoptosis rate.

Description

technical field [0001] The invention relates to a preparation method of liposome, in particular to a preparation method of ergosterol combined with gefitinib compound liposome freeze-dried powder, liposome and application. Background technique [0002] Due to the continuous increase in the incidence and mortality of cancer, it is still one of the most concerned problems in the world. According to statistics, in 2015, there were about 4.3 million new cancer cases in China, and about 2.81 million cancer patients died, among which lung cancer took the first place. The Global Cancer Incidence and Mortality Estimates 2018, produced by the International Agency for Research on Cancer, provides a status report on the global burden of cancer with an estimated 18.1 million new cancer cases and 9.6 million cancer cases in 2018 fatal cases. Lung cancer was the most common cancer (11.6% of all cases) and the leading cause of cancer death (18.4% of all cancer deaths). Clinically, it ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61K9/19A61K47/10A61K47/26A61K9/127A61K47/64A61P35/00A61P35/04
CPCA61K31/575A61K31/5377A61K9/19A61K47/26A61K47/10A61K9/127A61K47/64A61P35/00A61P35/04A61K2300/00
Inventor 黄绳武黄挺吴梅佳
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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