Traditional Chinese medicine compound polysaccharide with function of synergistically enhancing humoral and cellular immunity and application thereof

A compound polysaccharide and cellular immunity technology, which is applied in the fields of animal pharmacy, medicine and veterinary medicine, can solve the problems of mixed infection of bacterial diseases, destruction of the immune system of pigs, failure of vaccine immunity, etc., to increase the percentage of peripheral blood lymphocytes, strengthen spleen Lymphocyte proliferative ability and effect of enhancing ability

Active Publication Date: 2014-02-05
JINZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally recognized that immunosuppressive diseases such as porcine reproductive and respiratory disease syndrome, circovirus disease, pseudorabies, mycoplasma pneumonia, etc. can destroy the immune system of pigs, resulting in varying degrees of immunosuppression, in addition to easily causing mixed infections of bacterial diseases or secondary infection, more serious is the failure of related vaccine immunization

Method used

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  • Traditional Chinese medicine compound polysaccharide with function of synergistically enhancing humoral and cellular immunity and application thereof
  • Traditional Chinese medicine compound polysaccharide with function of synergistically enhancing humoral and cellular immunity and application thereof
  • Traditional Chinese medicine compound polysaccharide with function of synergistically enhancing humoral and cellular immunity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025]1) Take 2Kg of fresh shiitake mushrooms and wash them with water, add 20Kg of distilled water to decoct for 1 hour according to the mass ratio, take the primary filtrate of shiitake mushrooms and collect the medicinal residues;

[0026] 2) Take 0.5Kg Astragalus Chinese herbal decoction pieces, add 5Kg distilled water according to the mass ratio and ultrasonically impregnate for 20 minutes, decoct for 1 hour, take the primary filtrate of shiitake mushrooms and collect the medicinal residues;

[0027] 3) Take 0.5Kg Codonopsis pilosula Chinese herbal decoction pieces, add 5Kg distilled water according to the mass ratio and ultrasonically impregnate for 20 minutes, decoct for 1 hour, take the primary filtrate of Codonopsis pilosula and collect the dregs;

[0028] 4) The medicinal residues in steps 1), 2), and 3) were added to 5 times the amount of distilled water according to the mass ratio and decocted for 30 minutes to obtain the secondary filtrates of mushrooms, astragalus...

experiment example 1

[0033] Experimental example 1: CCK8 method was used to detect the effect of polysaccharides of single traditional Chinese medicine and polysaccharides of compound traditional Chinese medicine on the proliferation of mouse spleen lymphocytes in vitro

[0034] Test method: Aseptically take male Balb / c mouse spleen with an age of 6-8 weeks and a body weight of 20g±2g, and blow out the cells in RPMI-1640 culture medium. Collect the cell suspension and use Tris-NH4Cl to remove red blood cells, resuspend the cell pellet with RPMI-1640 culture medium, count more than 95% of viable cells by Trypan Blue staining, and adjust the cell density to 5×10 6 cells / ml to make a spleen cell suspension. The grouping and drug administration are shown in Table 1. After the above cells and drugs were added and marked, the 96-well cell culture plate was placed at 37°C and 5% CO 2 Continuously culture in the incubator for 44 hours; take out the culture plate, add 20 μl CCK8 solution to each well, an...

experiment example 2

[0045] Experimental Example 2: Using Orthogonal Experiments to Screen the Optimal Ratio of Chinese Medicine Combination Stimulating Spleen Lymphocyte Proliferation in Mice

[0046] Aseptically take the spleen of healthy 6-8 week old Balb / c male mice and blow out the cells in RPMI-1640 culture medium. Collect the cell suspension and use Tris-NH4Cl to remove red blood cells, resuspend the cell pellet with RPMI-1640 culture medium, count more than 95% of viable cells by Trypan Blue staining, and adjust the cell density to 5×10 6 cells / ml to make a spleen cell suspension. According to the results in Table 2, the optimal concentrations of lentinan, astragalus polysaccharide, and Codonopsis polysaccharide to stimulate the proliferation of mouse spleen lymphocytes in vitro are 25 mg / L, 25 mg / L, and 100 mg / L, respectively, adding 100 μl; using orthogonal experiments to design polysaccharides Combine and add cells, add cells and drugs, after marking, place the 96-well cell culture pla...

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Abstract

The invention relates to traditional Chinese medicine compound polysaccharide with a function of synergistically enhancing humoral and cellular immunity. The traditional Chinese medicine compound polysaccharide comprises the following components in parts by mass: 25 parts of lentinan, 50 parts of astragalus polysaccharide and 125 parts of odonopsis pilosula polysaccharide. The optimal dosage of the traditional Chinese medicine compound polysaccharide for oral administration of a mouse is 1 g per kilogram of body weight per day, and the mouse is continuously dosed for 7-10 days; the optimal dosage of the traditional Chinese medicine compound polysaccharide of a pig is 0.111 g per kilogram of body weight per day, and the pig is continuously dosed for 14-21 days. The three polysaccharides are scientifically and reasonably matched and combined to generate complementary and synergetic enhancement functions, the formed polysaccharide composition can comprehensively recover and enhance the immunity of organism, thereby having an obvious immunity enhancement effect, and the components of the traditional Chinese medicine compound polysaccharide are common raw medicinal materials, thereby being good in safety and free of toxic side effect.

Description

technical field [0001] The invention relates to the fields of animal pharmacy, medicine and veterinary medicine, in particular to a traditional Chinese medicine compound polysaccharide composed of fungal polysaccharides and plant polysaccharides that can not only enhance humoral immunity, but also synergistically enhance cellular immunity and its application. Background technique [0002] The immune function is the main force of the body's defense and disease resistance. The immune system is divided into non-specific immunity and specific immunity, the two interact and complement each other; non-specific immunity is the first barrier, and specific immunity is a more targeted and stronger immune response after non-specific immunity. A more effective defense mechanism to remove viruses or bacteria. Specific immunity includes humoral immunity and cellular immunity, both of which cooperate with each other to exert immune effects; in the process of virus infection, humoral immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/716A61K31/715A61P37/04
Inventor 周铁忠刘英姿蒋红王宏军高慎阳
Owner JINZHOU MEDICAL UNIV
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