Method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography

Abstract

The invention discloses a method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography. The method comprises the following stepsof: 1) respectively preparing aucubin, geniposidic acid and chlorogenic acid reference substance solutions, 2) respectively preparing to-be-detected sample solutions of Eucommia ulmoides Oliv leavesand barks, and 3) respectively sucking proper amounts of the reference substance solutions and the to-be-detected sample solutions by using a quantitative capillary tube, applying the solutions on a GF 254 silica gel plate, taking butyl acetate: methanol: formic acid as a mixed solvent as a developing solvent, observing through a 254 nm ultraviolet lamp, carrying out thin-layer chromatography identification by taking a 5.0% sulfuric acid-ethanol solution as a color developing agent, and observing the positions and the colors of spots appearing in the thin-layer chromatography silica gel plateof the sample solution. Results show that three active ingredients of Eucommia ulmoides Oliv leaves and barks in an obtained chromatogram show different spots. The identification method is high in specificity and convenient to operate, and can be used for rapidly and accurately identifying aucubin, geniposidic acid and chlorogenic acid in Eucommia ulmoides Oliv leaves and barks at the same time.

Description

technical field

[0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and in particular relates to a method for quickly identifying three active components of Eucommia ulmoides leaves and bark by using thin-layer chromatography. Background technique

[0002] Eucommia ulmoides Oliv.leaves are the dried leaves of Eucommia ulmoides, a plant of the genus Eucommia in the Eucommia family. They are mainly collected in summer and autumn when the branches and leaves are flourishing. Modern research shows that the chemical composition of Eucommia ulmoides leaves and bark is relatively similar, and some of them can be replaced by leaves. Eucommia ulmoides leaves are rich in active ingredients such as polyphenols, iridoids, and flavonoids, which have active effects such as lowering blood pressure, lowering blood lipids, enhancing immunity, anti-inflammation, anti-oxidation, and antibacterial, and have high development value. E...

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