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Method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography

A technology of thin-layer chromatography and active ingredients, which is applied in the field of identification of Chinese medicinal materials, to achieve the effect of convenient operation and strong specificity

Active Publication Date: 2019-12-31
INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although from a technical point of view, the identification and distinction of the three compounds in Eucommia leaves and bark can be achieved through sophisticated instrument analysis (such as high performance liquid chromatography, etc.), there is no useful, simple, efficient and accurate method to realize the identification of Eucommia ulmoides Simultaneous identification and differentiation of three characteristic components in leaves and bark

Method used

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  • Method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography
  • Method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography
  • Method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography

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Embodiment 1

[0037] A method for quickly identifying three active components of Eucommia ulmoides leaves and bark by thin-layer chromatography, comprising the following steps:

[0038] (1) Preparation of reference substance solution

[0039] Accurately weigh 10.1mg of aucubin reference substance, 10.0mg of genipinic acid reference substance and 10.3mg of chlorogenic acid reference substance in sequence, fully dissolve them in methanol solution with a volume fraction of 60%, and dilute to 10mL to prepare A standard reference solution with a concentration of aucubinin of 1.01 mg / mL, a concentration of geniposide of 1.00 mg / mL and a concentration of chlorogenic acid of 1.03 mg / mL.

[0040] (2) Preparation of eucommia leaves and bark to be tested sample solution

[0041] Fresh Eucommia leaves and bark were collected in July-August, and after microwave killing, they were dried at 45°C at low temperature to remove impurities and stored in a dry place away from light; the dried Eucommia leaves and...

Embodiment 2

[0056] A method for quickly identifying three active components of Eucommia ulmoides leaves and bark by thin-layer chromatography, comprising the following steps:

[0057] (1) Preparation of reference substance solution

[0058] Accurately weigh 10.1mg of aucubin reference substance, 10.0mg of genipinic acid reference substance and 10.3mg of chlorogenic acid reference substance in turn, fully dissolve them in methanol solution with a volume fraction of 100%, and dilute to 10mL to prepare A standard reference solution with a concentration of aucubinin of 1.01 mg / mL, a concentration of geniposide of 1.00 mg / mL and a concentration of chlorogenic acid of 1.03 mg / mL.

[0059] (2) Preparation of eucommia leaves and bark to be tested sample solution

[0060] Fresh Eucommia leaves and bark were collected in July-August, and after microwave killing, they were dried at 60°C to remove impurities and stored in a dry place away from light; the dried Eucommia leaves and bark were pulverize...

Embodiment 3

[0075] A method for quickly identifying three active components of Eucommia ulmoides leaves and bark by thin-layer chromatography, comprising the following steps:

[0076] (1) Preparation of reference substance solution

[0077] Accurately weigh 10.1mg of aucubin reference substance, 10.0mg of genipinic acid reference substance and 10.3mg of chlorogenic acid reference substance in sequence, fully dissolve them in ethanol solution with a volume fraction of 60%, and dilute to 10mL to prepare A standard reference solution with a concentration of aucubinin of 1.01 mg / mL, a concentration of geniposide of 1.00 mg / mL and a concentration of chlorogenic acid of 1.03 mg / mL.

[0078] (2) Preparation of eucommia leaves and bark to be tested sample solution

[0079] Fresh Eucommia leaves and bark were collected in July-August. After microwave killing, they were dried at 50°C at low temperature to remove impurities and stored away from light. The dried Eucommia leaves and bark were pulveri...

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Abstract

The invention discloses a method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography. The method comprises the following stepsof: 1) respectively preparing aucubin, geniposidic acid and chlorogenic acid reference substance solutions, 2) respectively preparing to-be-detected sample solutions of Eucommia ulmoides Oliv leavesand barks, and 3) respectively sucking proper amounts of the reference substance solutions and the to-be-detected sample solutions by using a quantitative capillary tube, applying the solutions on a GF 254 silica gel plate, taking butyl acetate: methanol: formic acid as a mixed solvent as a developing solvent, observing through a 254 nm ultraviolet lamp, carrying out thin-layer chromatography identification by taking a 5.0% sulfuric acid-ethanol solution as a color developing agent, and observing the positions and the colors of spots appearing in the thin-layer chromatography silica gel plateof the sample solution. Results show that three active ingredients of Eucommia ulmoides Oliv leaves and barks in an obtained chromatogram show different spots. The identification method is high in specificity and convenient to operate, and can be used for rapidly and accurately identifying aucubin, geniposidic acid and chlorogenic acid in Eucommia ulmoides Oliv leaves and barks at the same time.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and in particular relates to a method for quickly identifying three active components of Eucommia ulmoides leaves and bark by using thin-layer chromatography. Background technique [0002] Eucommia ulmoides Oliv.leaves are the dried leaves of Eucommia ulmoides, a plant of the genus Eucommia in the Eucommia family. They are mainly collected in summer and autumn when the branches and leaves are flourishing. Modern research shows that the chemical composition of Eucommia ulmoides leaves and bark is relatively similar, and some of them can be replaced by leaves. Eucommia ulmoides leaves are rich in active ingredients such as polyphenols, iridoids, and flavonoids, which have active effects such as lowering blood pressure, lowering blood lipids, enhancing immunity, anti-inflammation, anti-oxidation, and antibacterial, and have high development value. E...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 王志宏彭密军王翔杨秋玲
Owner INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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