Metal organic framework material-enzyme compound as well as preparation method and application thereof
A metal-organic framework and composite technology, applied in biochemical equipment and methods, enzymes, immobilized enzymes, etc., can solve the problems of free enzyme inactivation, achieve high catalytic activity, reduce costs, good stability and dispersibility Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0029] One aspect of the present invention provides a method for preparing a metal-organic framework material-enzyme complex, comprising the following steps:
[0030] Dissolve antioxidant enzymes, transition metal ions and organic ligands in a solvent for co-precipitation reaction;
[0031] Wherein, the antioxidant enzyme is selected from the combined enzyme of superoxide dismutase and catalase, glutathione peroxidase or horseradish peroxidase, or glutathione reductase and glutathione peroxidase Combined enzymes of oxidase, glucose dehydrogenase or alcohol dehydrogenase;
[0032] The transition metal ion is derived from a soluble metal salt, and the organic ligand is an imidazole compound.
[0033] The metal-organic framework material-enzyme complex has a pH-dependent protein release mechanism, and at the same time relies on the proton sponge effect, and can effectively escape from acidic endosomes after ingestion into cells, thereby ensuring its stability under physiological...
Embodiment 1
[0055] (1) Prepare 1.25mol / L 2-methylimidazole aqueous solution, 0.31mol / L zinc nitrate aqueous solution, 2.5mg / mL SOD solution, and 7.0mg / mL CAT solution respectively, and ultrasonicate at room temperature for 10min until completely dissolved, wherein, The molar ratio of zinc ion to 2-methylimidazole is 1:40;
[0056] (2) Add 160 μL of zinc nitrate aqueous solution, 50 μL of SOD solution, and 100 μL of CAT solution to 1.6 mL of 2-methylimidazole aqueous solution at the same time, and stir for 30 minutes at room temperature with a magnetic stirrer at a speed of 600 rpm;
[0057] (3) Centrifuge the solution obtained in step (2) at a speed of 15,000 rpm for 5 minutes, remove the supernatant, resuspend with deionized water, and repeat washing twice;
[0058] (4) The precipitate obtained in step (3) was pre-frozen at -20°C for 12 hours, and freeze-dried for 24 hours to obtain a metal-organic framework material-enzyme complex, which was designated as SC@ZIF-8.
[0059] The scannin...
Embodiment 2
[0061] The preparation method of this example is basically the same as that of Example 1, except that the concentration of the 2-methylimidazole aqueous solution is 1.875 mol / L. Specific steps are as follows:
[0062] (1) Prepare 1.875mol / L 2-methylimidazole aqueous solution, 0.31mol / L zinc nitrate aqueous solution, 2.5mg / mL SOD solution, and 7.0mg / mL CAT solution respectively, and ultrasonicate at room temperature for 10min until completely dissolved, wherein, The molar ratio of zinc ion to 2-methylimidazole is 1:60;
[0063] (2) Add 160 μL of zinc nitrate aqueous solution, 50 μL of SOD solution, and 100 μL of CAT solution to 1.6 mL of 2-methylimidazole aqueous solution at the same time, and stir for 30 minutes at room temperature with a magnetic stirrer at a speed of 600 rpm;
[0064] (3) Centrifuge the solution obtained in step (2) at a speed of 15,000 rpm for 5 minutes, remove the supernatant, resuspend with deionized water, and repeat washing twice;
[0065] (4) The pre...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com