Kit for determining glutathione reductase

A technology of glutathione and oxidized glutathione, which is applied in the field of medical testing, can solve problems such as affecting accuracy, poor anti-hemoglobin interference ability, and decreased enzyme activity, so as to reduce interference, improve clinical detection accuracy, The effect of improving detection accuracy

Active Publication Date: 2022-01-28
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commercially available GR detection reagents generally have the problem of poor anti-hemoglobin interference ability, and the result deviation is relatively large when detecting abnormal hemoglobin samples. Aggregation of form-forming molecules reduces enzyme activity, thereby affecting detection accuracy

Method used

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  • Kit for determining glutathione reductase
  • Kit for determining glutathione reductase
  • Kit for determining glutathione reductase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 GR detection kit

[0021] The glutathione reductase detection kit of the present invention comprises two liquid components of reagent R1 and reagent R2 which are independent of each other.

[0022] 1. Preparation of Reagent R1

[0023] Prepare according to the following formula, stir and mix thoroughly, and store at 2-8°C.

[0024]

[0025] 2. Preparation of Reagent R2

[0026] Prepare according to the following formula, stir and mix thoroughly, and store at 2-8°C.

[0027]

[0028]

Embodiment 2

[0029] The usage method of embodiment 2 kit

[0030] In this embodiment, a fully automatic biochemical analyzer (AU480) is used in conjunction with the kit of the present invention for sample detection, and the specific usage method is as follows:

[0031] (a) Mix the sample to be tested, the reagent R1 and the reagent R2 in a ratio of 10:250:50 to make them fully react;

[0032] (b) Use a fully automatic biochemical analyzer to measure the absorbance difference after the reaction (AU480: main wavelength 340nm, secondary wavelength 405nm, instrument reading point 14-27)

[0033] (c) Calculate the concentration of GR in the sample according to the absorbance change value.

[0034] Test principle: NADPH supplies hydrogen, glutathione reductase (GR) catalyzes the reduction of oxidized glutathione (GSSG) to generate reduced glutathione (2GSH), NADPH is oxidized to NADP+, causing absorbance at 340nm wavelength The rate of decline is proportional to the activity of GR in the sampl...

Embodiment 3

[0037] The performance test of embodiment 3 kits

[0038] The performance test of the glutathione reductase detection kit prepared in Example 1 was performed according to the method described in Example 2.

[0039] (1) Accuracy verification

[0040] Use the kit to test the accuracy of the quality control product, set 3 repetitions, read the signal through the automatic biochemical analyzer (AU480), calculate the relative deviation between the measured mean value and the target value for accuracy verification. The test results are shown in the table below:

[0041] Table 1 Accuracy Verification

[0042]

[0043]

[0044] From the above experimental results, it can be seen that the relative deviations between the test value and the target value are -0.84% ​​and 0.99%, respectively, both less than 1.0%. The experimental results show that the glutathione reductase detection kit of the present invention has better accuracy.

[0045] (2) Precision verification

[0046] Sel...

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Abstract

The invention discloses a kit for determining glutathione reductase. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, oxidized glutathione, a surfactant and EGTA, and the reagent R2 comprises a buffer solution, a reducing coenzyme II tetrasodium salt and a surfactant. By adding quantitative polyoxyethylene ether Brij58 into the reagent R1 and the reagent R2, interference of hemoglobin can be greatly reduced, the anti-interference capability reaches 5.0 g / L, and the detection accuracy of a hemoglobin abnormal sample is greatly improved; meanwhile, quantitative EGTA is added into the reagent R1, so that the problem that the enzyme activity is reduced due to the fact that glutathione reductase in a fresh sample mainly exists in a polymerase form is solved, and the clinical detection accuracy of the kit is effectively improved.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a kit for measuring glutathione reductase Background technique [0002] Glutathione reductase (GR) is one of the most important enzymes in the redox system of the human body. It is the main flavoenzyme that maintains the content of reduced glutathione (GSH) in cells. Under the catalysis of GR, the oxidized glutathione Glutathione reacts with reduced coenzyme II (NADPH), and then converts into reduced glutathione, which plays an important role in oxidative decomposition of hemoglobin, maintenance of thiol protein activity, and guarantee of thiol protein reducibility and cell integrity. important role. The clinical significance of serum glutathione reductase mainly includes: (1) An indicator for judging early liver damage, wherein early damage can be divided into physiological damage such as exercise-induced and pathological damage, such as acute hepatitis, drug-induced liver injury...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 李元丽刘霖杨焜诚
Owner ZYBIO INC
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