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Glutathione reductase testing reagent quality control product and preparation method

A technology of glutathione and reductase, applied in the field of biochemical detection, can solve the problems of affecting the detection results, rapid deterioration, and difficult preservation of liquid quality control products, and achieve the effect of maintaining overall activity, easy preservation, and prolonging quality.

Pending Publication Date: 2020-01-07
江西乐成生物医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the high precision and stability requirements of quality control products, the existing liquid quality control products are not easy to store, and will quickly deteriorate after opening, seriously affecting the test results

Method used

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  • Glutathione reductase testing reagent quality control product and preparation method
  • Glutathione reductase testing reagent quality control product and preparation method
  • Glutathione reductase testing reagent quality control product and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A quality control product for the determination of glutathione reductase, including freeze-dried products of the following components: 50 parts of phosphate buffer, 5 parts of glycine, 1 part of xanthan gum, 5 parts of bovine serum, pure glutathione 0.1 part of peptide reductase, 2 parts of sucrose, 1 part of preservative.

[0038] The advantages of the present invention are that phosphate buffer can facilitate the preparation and shaping of quality control products, preservatives extend the quality of quality control products, sucrose can prevent the inactivation of pure glutathione reductase during the preparation process, and glycine and xanthogen Glue can maintain the overall activity of quality control products.

[0039] Specifically, the phosphate buffer includes disodium hydrogen phosphate and sodium dihydrogen phosphate in a mass ratio of 2:3, and the preservative includes sodium azide and sodium glutamate in a mass ratio of 3:1.

[0040] It should be noted that a mix...

Embodiment 2

[0057] A quality control product for the determination of glutathione reductase, including freeze-dried products of the following components: 80 parts of phosphate buffer, 10 parts of glycine, 3 parts of xanthan gum, 10 parts of bovine serum, pure glutathione 0.5 part of peptide reductase, 5 parts of sucrose, 2 parts of preservative.

[0058] Specifically, the phosphate buffer includes disodium hydrogen phosphate and sodium dihydrogen phosphate in a mass ratio of 2:6, and the preservative includes sodium azide and sodium glutamate in a mass ratio of 3:2.

[0059] The preparation method of the above-mentioned glutathione reductase determination reagent quality control product includes the following steps:

[0060] (1) Take the pure glutathione reductase and add phosphate buffer to dilute to obtain a dilution;

[0061] (2) Add glycine to the diluent, keep the solution at 40°C, stir at a constant temperature for 30 minutes, and divide the resulting mixed solution into a first mixed solut...

Embodiment 3

[0073] A quality control product for the determination of glutathione reductase, including freeze-dried products of the following components: 55 parts of phosphate buffer, 5 parts of glycine, 2.5 parts of xanthan gum, 9 parts of bovine serum, pure glutathione 0.5 part of peptide reductase, 5 parts of sucrose, 1 part of preservative.

[0074] The advantages of the present invention are that phosphate buffer can facilitate the preparation and shaping of quality control products, preservatives extend the quality of quality control products, sucrose can prevent pure glutathione reductase from inactivation during the preparation process, glycine and xanthogen Glue can maintain the overall activity of quality control products.

[0075] Specifically, the phosphate buffer includes disodium hydrogen phosphate and sodium dihydrogen phosphate in a mass ratio of 2:5, and the preservative includes sodium azide and sodium glutamate in a mass ratio of 3:1.

[0076] The preparation method of the ab...

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Abstract

The invention discloses a glutathione reductase testing reagent quality control product which is characterized by comprising freeze dried products of the following components: 50-80 parts of a phosphate buffer, 5-10 parts of glycine, 1-3 parts of xanthan gum, 5-10 parts of bovine serum, 0.1-0.5 part of a pure product of glutathione reductase, 2-5 parts of cane sugar and 1-2 parts of a preservative. The quality control product is a freeze dried product, is capable of greatly improving the stability of the quality control product before use and is easy to preserve. The invention further discloses a preparation method of the quality control product. The preparation method comprises the following steps: adding the pure product of glutathione reductase into the phosphate buffer so as to obtaina diluted liquid; adding the glycine into the diluted liquid, performing heat-preservation stirring, and equally dividing the obtained mixed solution into a first mixed liquid and a second mixed liquid; adding the bovine serum and the cane sugar into the first mixed liquid so as to obtain a first freeze dried liquid; adding the bovine serum, the xanthan gum and the cane sugar into the second mixedliquid so as to obtain a second freeze dried liquid; and mixing the first freeze dried liquid with the second freeze dried liquid, and performing freeze drying, so as to obtain the quality control product.

Description

Technical field [0001] The invention relates to the technical field of biochemical detection, in particular to a quality control product of a glutathione reductase assay reagent and a preparation method. Background technique [0002] The blood contains glutathione reductase (GR), and its content needs to be determined with a glutathione reductase assay reagent. [0003] In the process of producing reagents for the determination of glutathione reductase, calibrators need to be used to calibrate the samples. The calibrators can be used to generate a calibration system, and then quality control products are used to test whether the calibration system is qualified. High precision and stability. At present, the quality control products are mostly the same components as the calibrators, and the components are pure liquid glutathione reductase, buffer and stabilizer. [0004] Due to the high accuracy and stability requirements of the quality control products, the existing liquid quality c...

Claims

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Application Information

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IPC IPC(8): C12Q1/26
CPCC12Q1/26
Inventor 万蒙邱家明周惠良
Owner 江西乐成生物医疗有限公司
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