Gene ZmSmk3 for encoding corn mTERF protein, cloning method and application thereof

A cloning method, corn technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems that the function of mTERF protein has not yet been resolved
CN107475262AActive Publication Date: 2017-12-15HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2017-12-15

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Abstract

The invention relates to a gene ZmSmk3 for encoding corn mTERF protein and a cloning method for the gene ZmSmk3. The cloning method comprises the following steps of: respectively extracting DNA of a plurality of homozygous mutants and homozygous wild type plant leaves, mixing the extracted DNA of the homozygous mutants and constructing a DNA pool of the homozygous mutants; mixing the extracted wild type DNA and constructing a mild type DNA pool; respectively carrying out heat-asymmetric staggering PCR (Polymerase Chain Reaction) on the constructed DNA pool of the homozygous mutants and the wild type DNA pool to obtain PCR product segments; detecting obtained PCR amplification product segments by using agarose gel electrophoresis, recovering specific bands generated in the DNA pool of the homozygous mutants, and carrying out sequencing to obtain a sequence of the mutant gene ZmSmk3. The mutation of the gene ZmSmk3 for encoding the corn mTERF protein has an influence on the seed development progress, and the gene has an important guidance significance for breeding practice.
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Description

technical field

[0001] The invention relates to the technical field of plant genetic engineering, in particular to a gene ZmSmk3 encoding a corn mTERF protein and a cloning method and application thereof. Background technique

[0002] Mitochondria are important energy conversion stations, which can efficiently convert the energy stored in organic matter into ATP (adenosine triphosphate), the direct energy source of cell life activities, and provide energy sources for life activities (Mackenzie and McIntosh, 1999; Gualberto et al., 2014). It has autonomous DNA replication, transcription and protein synthesis systems and is a semi-autonomous organelle. Among them, most mitochondrial proteins are encoded by the nucleus and are regulated by the nucleus.

[0003] Mitochondrial gene expression is a complex biological process, including RNA (ribonucleic acid) transcription, RNA editing, intron splicing, RNA translation, maturation, and degradation (Hammani and Giegé, 2014). The m...

Claims

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