Method for screening high-contribution pathogenic gene of rheumatoid arthritis

Abstract

A method for screening a high-contribution pathogenic gene of rheumatoid arthritis comprises: setting a rheumatoid arthritis patient group and a healthy contrast group, each group comprising a plurality of peripheral-blood specimens, separated from the human body, of different human bodies; extracting DNA from the peripheral-blood specimens; preparing DNA pools of the rheumatoid arthritis patient group and the healthy contrast group; determining the content, quality and integrity of the DNA pools, and if the DNA pools are usable, carrying out the next step; building a DNA library; adopting a whole genome resequencing method to sequence the rheumatoid arthritis patient group and the healthy contrast group, and obtaining a rheumatoid arthritis patient group sequencing result and a healthy contrast group sequencing result; and comparing the rheumatoid arthritis patient group sequencing result with the healthy contrast group sequencing result, and screening high-contribution genovariation sites out. According to the method, high-contribution pathogenic genovariation sites are screened, and the method is beneficial for early stage treatment and prevention of rheumatoid arthritis.

Description

technical field [0001] The present invention relates to a method for obtaining high-contribution pathogenic genes of rheumatoid arthritis as an intermediate result, in particular to a screening method for high-contribution pathogenic genes of rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune disease with a high global incidence rate, among which the incidence rate in my country remains high, and the number of female patients is much higher than that of males. It has always been the biggest and most common problem that plagues people's normal life. One of the common diseases, it is mainly characterized by joint symmetry and destruction, which erodes the bones and joints of the human body. In mild cases, temporary pain, inconvenient activities, and inflexibility of limbs appear. In severe cases, walking difficulties, bone deformities, etc. Accompanied by painful irreversible pathological features, and causing multiple inflammations of...

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Problems solved by technology

[0003] Rheumatoid arthritis has an inseparable relationship with microbial infection. Any bacterial invasion and infection will aggravate the pathological changes of rheumatoid arthritis. This may be caused by the secretion of certain substances after the invasion of bacteria that changes the protein structure. In this case, in recent years, we have extracted a large number of viruses such as herpes virus, staphylococcus, and zoster virus in the joints of patients with rheumatoid arthritis, which has attracted enough attention, and we have initially concluded that some bacteria and viruses can cause, Changing the pathological characteristics of rheumatoid arthritis and exacerbating the deterioration of the disease, not only that, a sampling survey experiment was carried out in the family of rheumatoid arthritis patients with obvious family genetic tendency, and it was found that the incidence of rheumatoid arthritis is high The gene is located in the 21-23 section of human chromosome 6, and there is a very important pathogenic factor called rheumatoid factor, which can be recognized by a protein antibody secreted by B cells to form a complex complement , if it enters the joints, it will be hydrolyzed by enzymes, and then destroy the joint organs to form the initial pathology of symptoms, but later found that the occurrence of this pathology is common between patients and healthy patients, so it cannot be included in the disease The other is that the DNA genome of patients with rheumatoid arthritis has obvious methylation after research, resulting in abnormal expression of many genes, which can affect the occurrence of joints in the body organs. The theory of rheumatoid arthritis has also produced several influential theories. It is believed that people with HLA-DR in different people's bodies have a stronger tendency to develop the disease, mainly because there are similar genes in this gene. The amino acid sequence is used as the receptor of the surface response mechanism, and the other is the elaboration of the LMP theory. The LMP-2 and 7 genes have polymorphisms, which are related to many diseases. Through the genetic variation of the LMP site, it is speculated that it is possible Linked to the pathogenesis of RA

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