RapMap method for rapid and high-throughput positioning and cloning of plant QTL gene

A high-throughput and fast technology, applied in genomics, bioinformatics, instruments, etc., can solve problems such as low efficacy and slow decay of local linkage disequilibrium, and achieve the goal of overcoming shortcomings and limitations, speeding up speed, and solving complexity Effect
CN111489790AActive Publication Date: 2020-08-04HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2020-08-04

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Abstract

The invention discloses a RapMap method for rapid and high-throughput positioning and cloning of a plant QTL gene. The method comprises the steps of: selecting strains with relatively small characterdifferences from the core germplasm as gradient parents; constructing as many F2 gradient genetic populations as possible; preliminarily positioning QTL by performing chip detection or next-generationsequencing on two separated extreme phenotypic DNA pools of each group, verifying the QTL at the single plant level of each gradient group according to the co-separation criteria provided by the invention, and cloning a target gene by using a QTL heterozygous family as a near-isogenic line for fine positioning. The concept of gradient genetic populations and the idea of co-segregation criteria are the core of the invention. Six rice grain length and grain width genes are successfully cloned by applying the RapMap method, and the RapMap is indicated to be a rapid and high-throughput gene cloning RapMap method integrating QTL positioning, verification and near-isogenic line screening into a whole.
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Description

technical field

[0001] The invention belongs to the field of plant functional genome and genetic engineering, and in particular relates to the field of positioning, cloning and application of fast and high-throughput QTL genes. Background technique

[0002] The large amount of natural genetic variation produced by crops or livestock in the process of natural and human selection is the basis and driving force for their domestication and improvement, their environmental adaptability and maintenance of genetic diversity, meeting human energy and health needs, and their production , quality, resistance, and formulation of molecular trait improvement strategies (Ellegren&Galtier, 2016; Rheenen et al., 2019; Mitchell-Olds, 2007). The regulation of multiple genes and the influence of the environment cause most economically important traits in biology, biomedicine and agriculture to be complex quantitative traits with continuous variation (Mauricio, 2001; Goddard & Hayes, 2009; Baza...

Claims

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