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Method for detecting Indel markers of beef cattle PLAG1 genes and special kit thereof

A beef cattle and gene technology, applied in the field of molecular genetics research, can solve the problems of high cost, achieve high accuracy, avoid specificity and complexity, and have good stability

Inactive Publication Date: 2018-05-04
NORTHWEST A & F UNIV
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Problems solved by technology

There are also studies using sequencing technology for InDel typing, the error rate is much lower than 1% (Bhangale et al, 2005), but the cost is high

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  • Method for detecting Indel markers of beef cattle PLAG1 genes and special kit thereof
  • Method for detecting Indel markers of beef cattle PLAG1 genes and special kit thereof
  • Method for detecting Indel markers of beef cattle PLAG1 genes and special kit thereof

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0027] The present invention utilizes PCR to detect the Indel marker of Jiaxian Red Cattle PLAG1 gene and is used for molecular breeding, usually comprising the following steps:

[0028] (1) Use the NCBI database to find the PLAG1 gene sequence, then use the Primer5 software to design primers, and use PCR to detect the primers;

[0029] (2) Use ordinary PCR technology and agarose gel electrophoresis to detect the typing of candidate sites in the population, and mark different individuals;

[0030] (3) Using SPSS 20.0 software to conduct correlation analysis between different typing types and cattle growth traits;

[0031] (4) Breed cattle with excellent growth traits according to different types.

[0032] 1. Cattle sample collection

[0033] The present invention specifically takes 190 Jiaxian Red Cattle as detection objects, among which the blood ...

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Abstract

The invention discloses a method for detecting Indel markers of beef cattle PLAG1 genes and a special kit thereof. The method comprises the following steps: based on a PCR (Polymerase Chain Reaction)technology, by taking a blood genome DNA pool of beef cattle (Jiaxian red cattle) as a template, and amplifying Indel sites of cattle PLAG1 genes by utilizing a specific primer pair P1; and performingagarose gel electrophoresis, and dividing different individuals into a deletion form, a normal form and a hybrid form according to electrophoresis results. The method provided by the invention is established on a relevance basis between the Indel sites of beef cattle PLAG1 genes and growth traits, and the method is simple, rapid and convenient to popularize and use and contributes to acceleratingmolecular marker-assisted selection of the beef cattle.

Description

technical field [0001] The invention belongs to the field of molecular genetics research, and specifically relates to a method for detecting PLAG1 gene Indel markers of beef cattle (Jixian Red Cattle). The method uses genomic DNA to carry out PCR, then performs agarose gel electrophoresis, and types according to the electrophoresis results. Background technique [0002] Genetic markers play a pivotal role in the establishment and development of genetics. With the further development of genetics and the emergence of molecular biology, genetic markers have successively experienced morphological markers, cytological markers, biochemical markers and DNA molecular markers. four stages of development. The first three stages are based on the results of gene expression and are an indirect reflection of genes. DNA molecular markers are a direct reflection of genetic variation at the DNA level, and can directly reflect differences at the DNA molecular level. In addition, they have hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 黄永震徐微郑立贺花张桂民陈宏雷初朝党瑞华蓝贤勇胡沈荣
Owner NORTHWEST A & F UNIV
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