Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Brassica napus wax powder gene positioning method based on whole genome resequencing

A Brassica napus, the whole genome technology, applied in the field of bioinformatics, can solve the problems of low detection efficiency and heavy workload, and achieve the effect of qualified quality and sufficient sequencing data

Active Publication Date: 2020-08-25
SHANGHAI ACAD OF AGRI SCI
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of mapping the wax powder gene of Brassica napus needs to detect all offspring individuals. This detection method has a large workload and low detection efficiency.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Brassica napus wax powder gene positioning method based on whole genome resequencing
  • Brassica napus wax powder gene positioning method based on whole genome resequencing
  • Brassica napus wax powder gene positioning method based on whole genome resequencing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The parental materials are the homozygous dimorphic line D0721, the temporary protection line D1577 and the restorer line D0936 grown in the Zhuanghang Experimental Base of the Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences.

[0061] 1) The sterile plant of the homozygous two-type line D0721 of Brassica napus was used as the mother, and the temporary insurance line D1577 was used as the male parent, and crossed to obtain a complete sterile line, a complete sterile line and a restorer line D0936 with no wax powder traits. Hybridization to obtain the F1 generation;

[0062] 3) From the 15:1 segregation population of the F2-F5 generation, the plants with the wax powder trait were selected for selfing to obtain the F6 generation.

Embodiment 2

[0064] 1 source of material

[0065] The test materials are the homozygous two-type line D0721, the temporary protection line D1577 and the restorer line D0936 planted in the Zhuanghang Experimental Base of the Institute of Crop Breeding and Cultivation of the Shanghai Academy of Agricultural Sciences. Wax plants and all with the wax phenotype did not segregate populations.

[0066] 2 methods

[0067] 2.1 Library construction and sequencing

[0068] The genomic DNA in the sample is extracted, and the library is constructed after the electrophoresis test is qualified. Qualified DNA samples were first randomly fragmented into 350bp fragments by Covaris, and the TruSeq DNA LT Sample Prepkit kit was used to build a library. The DNA fragments underwent end repair, poly(A) tailing, sequencing adapters, purification, and PCR amplification. and other steps, and finally complete the library construction, and use the sequencer to perform double-end sequencing after the library is qua...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a brassica napus wax powder gene positioning method based on whole genome resequencing, and belongs to the technical field of biological information. A near-isogenic line is established on the basis of a segregation population. Mixed grouping analysis is adopted, wax powder-free plants in a 3: 1 segregation population, all wax powder phenotypic insegregation populations andthree parents in a near-isogenic line are selected, and five DNA pools are built in total for genome resequencing. And genetic correlation analysis is performed by utilizing resequencing data, and a locus for controlling the character of the wax powder is positioned in a 590663-1657546bp region of an A08 chromosome. The method provided by the invention has the biggest characteristic that all offspring individuals do not need to be detected, but offsprings with two extreme characters are subjected to mixed pool analysis. Moreover, the method provided by the invention has sufficient sequencing data and qualified quality, and can be used for next analysis.

Description

technical field [0001] The invention relates to the technical field of biological information, in particular to a gene positioning method of brassica napus wax powder based on whole genome resequencing. Background technique [0002] Brassica napus L. is one of the three major types of rapeseed, which evolved from double diploidization of Chinese cabbage (AA, n=10) and Brassica napus (CC, n=9) through natural interspecific hybridization. A compound species. The surface of the plant is generally covered with a layer of wax. Wax is a mixture of long-chain fatty acids, which can prevent ultraviolet radiation damage, reduce excessive evaporation of water on the plant surface and protect plants from diseases and insect pests, and affect the photosynthetic efficiency and pollen fertility of plants. Generally speaking, the stems, leaves and siliques of Brassica napus are covered with wax powder, while the stems, leaves and siliques of the non-wax mutant are not covered with wax po...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858A01H1/02A01H1/04G16B30/10G16B50/30
CPCC12Q1/6895C12Q1/6858A01H1/02A01H1/04G16B30/10G16B50/30C12Q2600/156C12Q2600/13C12Q2535/122C12Q2537/165
Inventor 张俊英朱吉风周熙荣杨立勇江建霞蒋美艳李延莉王伟荣孙超才
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com