The invention relates to the field of computer image processing and map surveying and mapping, in particular to a multi-air strip video image splicing and positioning method based on multi-rotor unmanned aerial vehicle flight path constraint, which is mainly used for improving the splicing quality of the images between the air strips and the positioning precision of a panorama. The method comprises the following steps of firstly, processing the GPS information of all images in an aerial video, obtaining the flight path information, then performing a series of image processing operations on theto-be-spliced image to obtain a homography matrix, mapping the to-be-spliced image into the spliced image by utilizing the matrix, and finally realizing a GPS positioning function by utilizing a one-to-one correspondence relationship between the image and the GPS information. According to the invention, the image splicing effect under the condition that the plurality of air strips are parallel can be effectively improved, all scene information shot during the flight process of the unmanned aerial vehicle can be truly reflected by the obtained splicing result, in addition, the GPS coordinatesof each pixel point in the splicing result can be positioned by combining the GPS information acquired in the flight process, and the positioning error is not more than 10m.