The invention relates to a method for constructing and identifying 
molecular marker fingerprint chromatogram of 
Dendrobium huoshanense and similar species thereof, which solves the problems that a method of identifying 
medicinal plants in the prior art has high cost, complicated procedures and long time consumption. The method comprises the steps of: 1, the collection of 
Dendrobium huoshanense samples; 2 the extraction and purification of 
DNA of genomes of the samples; 3. ISSR-PCR amplification; 4, 
agarose gel electrophoresis; 4, the construction of ISSR 
molecular marker fingerprint chromatogram of the samples to be tested; and 5, the identification of 
Dendrobium huoshanense 
germplasm by utilizing the constructed ISSR 
molecular marker fingerprint chromatogram. The method has the advantages that the method saves the time and the cost, and can obtain an accurate and reliable identification chromatogram through the extraction to 
DNA of Dendrobium plants, the ISSR-PCR amplification, and the 
agarose gel electrophoresis; the method has the advantages of simplicity, convenience, quickness, good repetitiveness, and 
high resolution on the identification of raw materials which are easy to confuse in appearance; and the method can identify in a 
seedling stage, which has important effect on ensuring the accuracy and stability of base resources of medicinal materials.