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Processing method of comprehensive utilization of edible-medicinal fungi mushroom bran

The invention discloses a processing method of comprehensive utilization of edible-medicinal fungi mushroom bran. The processing method is characterized by comprising the following steps: firstly, performing innocent treatment on the mushroom bran, adding a protein material and a moisture conditioning agent required by the growth of livestock into the mushroom bran after the mushroom bran is subjected to enzymolysis digestion and concentration, preparing the mixture into probiotics protein feed products through compound bacteria fermentation, then enabling mushroom bran residues generated after digestion of the mushroom bran to be further subjected to cooperative fermentation with animal excreta and saccharomycetes, and adding complex microorganism bacterium after Maillard reaction, so as to obtain bio-organic fertilizer products. The method realizes thorough comprehensive utilization effect on the mushroom bran, provides a new method, process and technical route for comprehensive resource utilization of edible-medicinal fungi mushroom bran in China, changes wastes into valuables, reduces environmental protection, can further improve the livestock yield and the meat quality through the probiotics protein feed, and saves grain resources, and the bio-organic fertilizer products are particularly important in the aspects of improvement of soil environment, increase of the crop yield and promotion of agricultural green planting, and have obvious economic and social benefits.
Owner:邳州市东方养殖有限公司 +3

Construction and identification method of molecular marking fingerprint of Dendrobium huoshanense and similitude species thereof

The invention relates to a method for constructing and identifying molecular marker fingerprint chromatogram of Dendrobium huoshanense and similar species thereof, which solves the problems that a method of identifying medicinal plants in the prior art has high cost, complicated procedures and long time consumption. The method comprises the steps of: 1, the collection of Dendrobium huoshanense samples; 2 the extraction and purification of DNA of genomes of the samples; 3. ISSR-PCR amplification; 4, agarose gel electrophoresis; 4, the construction of ISSR molecular marker fingerprint chromatogram of the samples to be tested; and 5, the identification of Dendrobium huoshanense germplasm by utilizing the constructed ISSR molecular marker fingerprint chromatogram. The method has the advantages that the method saves the time and the cost, and can obtain an accurate and reliable identification chromatogram through the extraction to DNA of Dendrobium plants, the ISSR-PCR amplification, and the agarose gel electrophoresis; the method has the advantages of simplicity, convenience, quickness, good repetitiveness, and high resolution on the identification of raw materials which are easy to confuse in appearance; and the method can identify in a seedling stage, which has important effect on ensuring the accuracy and stability of base resources of medicinal materials.
Owner:陈乃富
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