The invention discloses a processing method of comprehensive utilization of edible-medicinal fungi mushroom bran. The processing method is characterized by comprising the following steps: firstly, performing innocent treatment on the mushroom bran, adding a protein material and a moisture conditioning agent required by the growth of livestock into the mushroom bran after the mushroom bran is subjected to enzymolysis digestion and concentration, preparing the mixture into probiotics protein feed products through compound bacteria fermentation, then enabling mushroom bran residues generated after digestion of the mushroom bran to be further subjected to cooperative fermentation with animal excreta and saccharomycetes, and adding complex microorganism bacterium after Maillard reaction, so as to obtain bio-organic fertilizer products. The method realizes thorough comprehensive utilization effect on the mushroom bran, provides a new method, process and technical route for comprehensive resource utilization of edible-medicinal fungi mushroom bran in China, changes wastes into valuables, reduces environmental protection, can further improve the livestock yield and the meat quality through the probiotics protein feed, and saves grain resources, and the bio-organic fertilizer products are particularly important in the aspects of improvement of soil environment, increase of the crop yield and promotion of agricultural green planting, and have obvious economic and social benefits.