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Kit for quickly detecting nitrite in edible bird's nest and application of kit in edible bird's nest detection

A nitrite and kit technology, which is applied in the direction of analysis by chemical reaction of materials and material analysis by observing the impact on chemical indicators, can solve problems such as product blanks in nitrite kits, and achieve sample Simple pretreatment, narrow detection range, and cost reduction

Active Publication Date: 2012-06-20
青岛正典生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic rapid detection methods and kits for nitrite in bird's nest are blank

Method used

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  • Kit for quickly detecting nitrite in edible bird's nest and application of kit in edible bird's nest detection
  • Kit for quickly detecting nitrite in edible bird's nest and application of kit in edible bird's nest detection
  • Kit for quickly detecting nitrite in edible bird's nest and application of kit in edible bird's nest detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Kit

[0052] see details figure 1 . Box body: ABS material; detection solution A: 10 ml; detection solution B: 10 ml; detection solution C: 50 ml; dropper: glass material, 2 ml; glass sample cup: 25 ml; transparent glass colorimetric tube: transparent Glass material, 10.2 ml (scaled capacity), size 1.2 cm×1.0 cm×8.5 cm (length×width×height), square column type, upper opening; transparent glass nitrite enrichment column: transparent glass material, 16 ml , with a size of 1.6 cm×8 cm (diameter×height), cylindrical, with a sand core filter layer at the lower end (see figure 2 ); the macroporous resin is made of domestic D201 macroporous resin; the sponge bracket is made of polyurethane sponge material; the color comparison card (see image 3 ) color scale ranges from 0.1-380 mg / kg.

Embodiment 2

[0053] Example 2 Preparation of three kinds of detection solutions

[0054] 1. Preparation of detection solution A (Griess solution A)

[0055] 4-aminobenzenesulfonic acid solution: Weigh 4 g of 4-aminobenzenesulfonic acid and dissolve it in 700 mL of water and 300 mL of glacial acetic acid, mix well in a brown reagent bottle, and store at room temperature.

[0056] 2. Preparation of detection solution B (Griess solution B)

[0057] N-1-naphthylethylenediamine solution (1 g / L): Weigh 0.1 g of N-1-naphthylethylenediamine, add 100 mL of 60% acetic acid to dissolve, mix well, and put it in a brown reagent bottle.

[0058] 3. Preparation of detection solution C

[0059] Mix 32 ml of sodium sulfate saturated solution and 68 ml of borax saturated solution, and put it in a brown reagent bottle.

Embodiment 3

[0060] The preparation of embodiment 3 color comparison card

[0061] (1) Reagent preparation

[0062] 1. Borax-sodium hydroxide solution (pH=10): Take 14.768 grams of borax, dissolve it in 250 ml of water, and prepare a borax solution with a concentration of 0.05 moles per liter; take 2.0 grams of sodium hydroxide, dissolve it in 250 ml of water, It is configured into a sodium hydroxide solution with a concentration of 0.2 moles per liter; 50 milliliters of 0.05 moles per liter of borax solution is mixed with 43 milliliters of 0.2 moles per liter of sodium hydroxide solution to obtain borax-hydroxide with a pH value of 10 sodium solution;

[0063] 2. Sodium nitrite standard solution: Accurately weigh 25.0 mg of sodium nitrite dried in a silica gel desiccator for 24 hours, add water to dissolve it and transfer it to a 500 mL volumetric flask, add 100 mL of borax-sodium hydroxide solution buffer, add water to dilute to the mark, Mix well and store at 4°C in the dark. This so...

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Abstract

The invention discloses a kit for quickly detecting nitrite in edible bird's nest, which comprises a box body and reagents, wherein a sponge bracket is arranged in the box body and provided with holes and grooves; a brown reagent bottle filled with Griess solution A ( i.e. detecting solution A), a brown reagent bottle filled with Griess solution B (i.e. detecting solution B), and a brown reagent bottle filled with protein precipitant (i.e. detecting solution C) are mounted and inserted into the holes in the sponge bracket; a glass sample cup, a dropper, a clear glass color comparison tube, and a clear glass nitrite enriching column are placed in the grooves in the sponge bracket; and a colorimetric card well sealed by a black plastic bag is placed in the box body. The kit can be applied to food detection, especially to the content detection of nitrite in the edible bird's nest. The kit disclosed by the invention and the application of the kit in edible bird's nest detection have the characteristics of less sample amount, low detection limit, wide detection range, simplicity in pretreatment of the sample, and the like.

Description

technical field [0001] The invention relates to the detection of nitrite, in particular to the detection of nitrite in food, in particular to a kit for rapidly detecting nitrite in bird's nest and its application in the detection of bird's nest. Background technique [0002] Nitrite is a general term for a class of inorganic compounds. Mainly refers to sodium nitrite (English name Sodium nitrite, CAS number: 7632-00-0, molecular formula: NaNO 2 ). Sodium nitrite is white to light yellow powder or granule, with slightly salty taste, easily soluble in water. The appearance and taste are similar to table salt, and it is widely used in industry and construction. [0003] Nitrite can make meat and meat products present a good color, and has anti-corrosion and fresh-keeping effects. It is often used in large quantities in the processing and production of meat products in our country. Due to the low price, nitrite is illegally used in many bird's nest products, many pickled ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 程金生
Owner 青岛正典生物科技有限公司
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