46results about How to "Good differential diagnosis" patented technology

The invention discloses a RPA primer for detecting chicken infectious laryngotracheitis virus. The nucleotide sequence of the RPA primer is shown as SEQ ID NO.1 and SEQ ID NO.2; the RPA primer is strong in specificity, high in sensitivity, and accurate in detection result. The invention further provides a RPA method for detecting the chicken infectious laryngotracheitis virus. The detecting method is easy to operate, low in equipment requirement, strong in specificity, high in sensitivity, and accurate and reliable in detection result; the detecting method provides a low-cost, fast and specific field diagnosis method for effectively detecting and diagnosing the chicken infectious laryngotracheitis virus.

The invention discloses a kit for identifying and diagnosing bovine theileria annulata, theileria sergenti and theileria sinensis sp.nov., a preparation method and an application method of the kit. The kit at least comprises an upstream primer of the theileria annulata and a downstream primer of the theileria annulata, an upstream primer of the theileria sergenti and a downstream primer of the theileria sergenti, as well as an upstream primer of the theileria sinensis sp.nov. and a downstream primer of the theileria sinensis sp.nov.

The invention provides a single-cylinder double-chamber injector needle cylinder; the needle cylinder is provided with two pistons which divide the needle cylinder into a front chamber and a back chamber; the two pistons are connected with each other by a flexible connecting piece; the front chamber piston is provided with a through hole and a sealing device, thus realizing double drug administration of CT and MR contrast agent and physiological saline; remaining contrast agent in the cylinder is injected into human body by a physiological saline washing pipe and enters vein after being driven, so that the contrast agent reaches the target organ rapidly, thus increasing contrast of pathological changes with surrounding tissues and improving checkout of the pathological changes; meanwhile, blood supply information of nidus is increased, which facilitates diagnosis and differential diagnosis and increases certainty factor of diagnosis by the doctors; when CT and MR angiogram is carried out, contrast between the blood vessel and surrounding tissues is increased, which improves the angiogram effect; perfusion imaging is carried out on CT and MR, so that the contrast agent which enters the targeted organ has more constant rate and more accurate data is obtained; on the basis, more abundant information is expected to be obtained with the same amount of contrast agent used or the use amount of the contrast agent is expected to be reduced.

The invention provides a primer group for identifying porcine circovirus types 1, 2 and 3 and application of the primer group. The sequences of the primer group are shown in SEQ ID NO.1 -4. On the basis of the genome structure characteristics of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus, genome specific conserved areas of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus are selected as upstream primer design areas, and downstream primers are designed separately in differential areas of genomes generated according to the three genotypes (PCV1, PCV2 and PCV3). The primer is simple in condition optimization, convenient and high in reaction speed, and a result is observed conveniently; an assembled kit can be used for rapid differential diagnosis of the three genotypes of porcine circovirus (PCV1, PCV2 and PCV3).

The invention discloses a kit, a primer pair, a probe and a method for detecting vibrio parahaemolyticus. The kit comprises an RPA reaction system which comprises an RPA primer probe mixture, the RPAprimer probe mixture comprises the primer pair and the probe, a nucleotide sequence of an upstream primer in the primer pair is shown as SEQ ID NO.1 while that of a downstream primer in the primer pair is shown as SEQ ID NO.2, and nucleotide sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer is high in specificity and sensitivity and accurate in detection result. By combining RPA technology for detecting vibrio parahaemolyticus with a colloidal gold flow measuring chromatography test paper strip, quick detection of vibrio parahaemolyticus can be realized, and 10pg of vibrio parahaemolyticus DNA can be detected at least.

The present disclosure provides methods and compositions that find use in facilitating a diagnosis of inflammatory liver disease in a subject. The methods and compositions generally involve detection of eotaxin-3 (E3) levels, either alone or with levels of eotaxin-1 (E1), and optionally, with levels of CCL22 and, further optionally, with levels of IL15. These levels can be used to facilitate a diagnosis of a liver disease of at least one of autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC), and / or to facilitate a differential diagnosis between AIH, PBC, and PSC. The methods and compositions of the present disclosure also find use in facilitating treatment decisions for a subject.

The invention discloses a rapid diagnostic kit for a uterine sarcoma in early and middle stages .The kit is prepared from CD10, death reporters and growth differentiation factors-15 .The invention further discloses a preparation method of the rapid diagnostic kit for the uterine sarcoma in the early and middle stages .By means of the rapid diagnostic kit for the uterine sarcoma in the early and middle stages, the uterine sarcoma can be detected more accurately and comprehensively, benign and malignant pelvic masses can be better judged, differential diagnosis is better conducted on gynecological benign uterine masses and uterine sarcomas, and the sensitivity and the accuracy of the kit both reach 70%.

The invention provides application of Mycobacterium tuberculosis protein in the preparation of products to diagnose patients with latent tuberculosis infection and / or active tuberculosis. A method provided herein can detect the levels of three different Mycobacterium tuberculosis protein serum antibodies at the same time, thereby better identifying and diagnosing patients with latent tuberculosisinfection and patients with active tuberculosis; the sensitivity is higher, and the specificity is better.

The invention discloses a recombinant expression vector for a lung fluke excretion and secretion protein, an engineering strain, a preparation method and an application thereof. The recombinant expression vector contains the encoding gene of the lung fluke excretion and secretion protein, the nucleotide sequence of the coding gene is shown as SEQ ID No.1, and the expression vector is pQE-80L. Theengineering strain contains the recombinant expression vector, and a host strain is bacillus coli. The method for preparing the lung fluke excretion and secretion protein through the recombinant expression vector comprises four steps of: constructing the engineering strain, culturing, inducting and expressing, and purifying an expression product. The lung fluke excretion and secretion protein canbe used for preparing agents or devices for detecting a paragonimiasis The recombinant expression vector and the engineering strain have the advantages of high expression amount and stable expression, and the lung fluke excretion and secretion protein prepared by the method has high protein yield and high immunoreactivity.

The invention discloses a porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of a cell and attenuation via drug screening and an application thereof. The attenuated vaccine strain is prepared through the following steps: based on a porcine pseudorabies virus variant strain (named as strain HeN1, of which the microbial preservation serial No. is CGMCC No. 6656), firstly, carrying out low-temperature passage and screening on a Vero cell to obtain large fragments of deleted viruses including gI, gE, Us9, Us2 and part of inverted repeated sequence which exist in zone US through, and then making the TK gene thereof partially deleted through drug screening. The gene-deleted attenuated vaccine strain is named as strain PRV TP, of which the microbial preservation serial No. is CGMCC No. 12300. A live vaccine or an inactivated vaccine (a single vaccine or combined vaccine) can be prepared from the attenuated vaccine strain disclosed by the invention, and can prevent porcine pseudorabies effectively, and a reagent for diagnosing or treating porcine pseudorabies can be prepared from the attenuated vaccine strain too. According to the porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of a cell and attenuation via drug screening and the application thereof, the porcine pseudorabies attenuated vaccine strain PRV TP has the advantages of good safety, efficient protection, convenient differential diagnosis and the like.

The invention provides application of mycobacterium tuberculosis protein in preparation of products for diagnosing latent tuberculosis infectors and active pulmonary tuberculosis. The method providedby the invention can be used for detecting the levels of three different mycobacterium tuberculosis protein serum antibody responses at the same time, so that latent tuberculosis infectors and activetuberculosis patients can be better identified and diagnosed, the sensitivity is higher, and the specificity is better.

The invention discloses a colloidal gold test strip for testing toxoplasma gondii antibodies.The colloidal gold test strip comprises a bottom board, a sample pad, a gold-labeled protein-enveloped conjugate pad, a nitrocellulose membrane containing a detection line T and a quality control line C and an absorbent pad, wherein the sample pad, the conjugate pad, the nitrocellulose membrane and the absorbent pad are disposed on the bottom board.Experiments prove that the colloidal gold test strip is capable of detecting serum antibodies of toxoplasma gondii in animal bodies and achieving differentiating diagnosis of protozoan infection of animals conveniently, cost saving and simple and convenient to operate and is applicable to the animals such as pigs, dogs and cats.

The invention discloses a multi-RPA primer and probe for simultaneously detecting a newcastle disease virus, an infectious bronchitis virus and an H9N2-subtype avian influenza virus. The multi-RPA primer and probe comprise a primer and probe for detecting the newcastle disease virus, a primer and probe for detecting the infectious bronchitis virus and a primer and probe for detecting the H9N2-subtype avian influenza virus. The multi-RPA primer and probe are high in specificity, high in sensitivity and accurate in detection result. The invention further discloses a method for simultaneously detecting the newcastle disease virus, the infectious bronchitis virus and the H9N2-subtype avian influenza virus through multi-RPA. The method comprises the steps of primer synthesis, template RNA extraction, reverse transcription, multi-PCR amplification and amplification product analysis. The detection method is simple in operation and good in stability, and a low-cost, fast and specific field diagnosis method is provided for effective detection and identification of mixed infection of common avian respiratory diseases.

The invention discloses a kit for rapidly detecting an animal hydatid natural infection antibody. The kit comprises the following raw materials in parts by weight: a reagent A including 20-30 mmol / L of a Hyd diluent, 90-110 mmol / L of a buffer solution, 0.03-0.05 mmol / L of a DNA sequence solution and 0.2-0.4 mmol / L of a developing solution A; a reagent B including 20 to 30mmol / L of Hyd diluent, 0.5to 0.7 mmol / L of Hyd enzyme conjugate, 90 to 110mmol / L of buffer solution and 0.1 to 0.3 mmol / L of developing solution B. The invention relates to the technical field of the detection of the naturalinfection antibody of the hydatid. The invention discloses the kit for rapidly detecting the animal hydatid natural infection antibody, the DNA sequence solution in the reagent A and a vaccine immuneantibody are used for recognition, the color development is carried out by using the color development solution A; then, the Hyd enzyme conjugate in the reagent B is used for being specifically combined with the hydatid natural infection antibody; the development solution B is matched for development such that the antibody generated by natural infection can be selectively detected, the interference of the vaccine immune antibody is avoided, the infection of hydatid disease can be quickly, simply, conveniently and accurately identified and diagnosed, and the problem that the antibody detectionreagent cannot distinguish the vaccine immune antibody from the antibody generated by natural infection is effectively avoided.

The invention discloses a kit, a primer pair, a probe and a method for detecting vibrio parahaemolyticus. The kit comprises an RPA reaction system which comprises an RPA primer probe mixture, the RPAprimer probe mixture comprises the primer pair and the probe, a nucleotide sequence of an upstream primer in the primer pair is shown as SEQ ID NO.1 while that of a downstream primer in the primer pair is shown as SEQ ID NO.2, and nucleotide sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer is high in specificity and sensitivity and accurate in detection result. By combining RPA technology for detecting vibrio parahaemolyticus with a colloidal gold flow measuring chromatography test paper strip, quick detection of vibrio parahaemolyticus can be realized, and 10pg of vibrio parahaemolyticus DNA can be detected at least.

The invention discloses an RPA (recombinase polymerase amplification) primer for detecting a fowl adenovirus serotype 4. The nucleotide sequence of the RPA primer is as shown in SEQ ID No.1 and SEQ ID No.2 in the description. The invention further discloses a method for detecting the RPA of the fowl adenovirus serotype 4, and the method mainly comprises the steps of primer synthesis, extraction of DNA in a sample to be tested, RPA amplification reaction and analysis of an RPA amplification product. According to the RPA primer disclosed by the invention, the specificity is strong, the sensitivity is high, and the detection result is accurate. The detection method disclosed by the invention is simple in operation and good in stability, and a low-cost, rapid and specific on-site diagnosis method for effectively detecting and authenticating chicken cecum hepatitis-hydropericardium syndrome is provided.