50results about How to "Easy to observe the results" patented technology

The invention belongs to the technical field of information processing, and discloses a sleep monitoring method and system, a storage medium, a computer program and a device. The method comprises thefollowing steps: initializing a FreeRTOS operating system, an IO port, a clock, WiFi, Bluetooth and an analog-to-digital converter; performing digital-to-analog conversion on the three paths of electroencephalogram signals, the two paths of electro-oculogram signals and the one path of electromyogram signal in sequence to obtain corresponding digital signals; judging whether the number of the acquired signal groups is full of the WiFi data packets, if not, directly sending Bluetooth data, and if so, sending the WiFi data, emptying the WiFi data packets and then sending the Bluetooth data packets; and carrying out digital-to-analog conversion on the data, alternately sending the data, and repeating until shutdown. The system comprises a physiological signal acquisition module, a physiological signal preprocessing module and a physiological signal analysis module. According to the invention, the sleep state of the user can be accurately and scientifically monitored, and the sleep qualitycondition of the user can be timely reported after WeChat applet processing.

The invention discloses a method for preparing transmission microscope samples on the basis of non-precision positioning, and belongs to the technical field of semiconductors. The method includes providing failure analysis chips and positioning and labeling failure points in the failure analysis chips to obtain labeled zones; cutting a side of each labeled zone to form first wedge-shaped cavitiesand thinning the sections, which are close to the labeled zones, of the first wedge-shaped cavities until the failure points can be observed; forming protective layers on the sections where the failure points are observed; cutting the other side of each labeled zone to form second wedge-shaped cavities and thinning the sections, which are close to the labeled zones, of the second wedge-shaped cavities until primary samples with preset thicknesses are obtained; carrying out U-shaped cutting-off on the bottoms of the primary samples and then thinning the primary samples to obtain the transmission microscope samples. The method has the advantages that the failure points can be effectively prevented from being cut by mistake, each cut surface with the corresponding failure points can be prevented from being damaged or stained by splashing in follow-up processes, accordingly, the sample preparation success rate can be increased, and observation results can be improved.

The quick method of identifying bovine mycobacterium and tubercle mycobacterium includes: adding bovine mycobacterium Vallee strain genome DNA to proliferate one 294 bp segment, detecting target gene from bovine mycobacterium DNA with bovine mycobacterium primer, adding tubercle mycobacterium H37vR strain genome DNA to proliferate one 294 bp segment, proliferating target genome from tubercle mycobacterium DNA with tubercle mycobacterium primer, adopting the same upstream primer and different downstream primers and adopting two gene segments of 294 bp and annealing temperature of 67 deg c. The method of present invention is used in the direct detection of bacterial cultured matter and clinical sample.

The invention discloses a soil detection sampling device, which comprises a shell. A display screen and a handrail are arranged on the surface and the top face of the shell; a button is arranged on the handrail; soil inlets I are symmetrically formed in the side faces of the shell; a movable ring is movably connected to the bottom face of the shell; a plurality of triangular teeth are fixedly connected to the bottom face of the movable ring; a spring is arranged in the shell; the bottom end of the spring is fixedly connected onto an inner wall of the bottom face of the shell; a ball screw is arranged in the shell; one end of the ball screw is fixedly connected with the movable ring; the other end of the ball screw sequentially penetrates through a soil sampler and a clapboard, and is fixedly connected with an output shaft of a motor located above the clapboard; the soil sampler is located above the spring; a nut I is fixedly connected to the middle part of the bottom face of the soil sampler. The soil detection sampling device has the beneficial effects of high safety performance, simplicity in operation, capability of effectively sampling deep soil, ideal detection result, and capability of effectively alleviating working pressure of people.

The invention aims at providing a novel excrement sampling and rapid immune detection device which is low in cost, convenient to manufacture and convenient for result observation and can avoid pollution and infection in a detection process, and an operation method thereof. The device comprises an excrement sampling pipe (1), an outer cover (2) and an excrement sampling rod (3), wherein the excrement sampling pipe (1) is composed of an upper pipe fitting (11) and a lower pipe fitting (12); the bottom of the upper pipe fitting (11) is connected with the outer side wall of the lower pipe fitting (12) to form a groove (4); the center of the excrement sampling rod (3) is hollow; and a through hole (5) which is communicated with the center of the excrement sampling rod (3) is formed in the outer cover (2). In the operation method of the device, the outer cover (2) does not need to be unscrewed in other steps except the steps of collecting a sample and putting a detection test strip; and dilution or cracking and detection of the sample can be both finished in the excrement sampling pipe (1). The novel excrement sampling and rapid immune detection device is applied to the technical field of a collection and detection device of the sample.

A method of accelerating sample treatment with addition of graphene belongs to the technical field of biological sample treatment and pathology. The invention particularly relates to the method of accelerating a sample treatment process with addition of graphene, by that the required time of the sample treatment process, such as dehydration, fixation, staining and the like. The method mainly solves a problem of a poor reliability of results since excessive long time of operation of sample treatment is required so that the sample in not fresh enough in the prior art. In the technical scheme, theoretical innovation of combining the new material, graphene, with the sample treatment is disclosed, namely, by means of the characters of adsorbing various atoms and molecules and being high in specific surface of the graphene, the sample treatment process is accelerated by carrying active components rapidly into the samples such as tissue, blood, cell and microorganisms and the like. The method is mainly used in treatment of various samples in clinical and scientific fields, comprising fixation, staining, blue returning, dehydration, transparence and sealing and the like on tissue samples (such as human, animal or plant samples) and fixation, staining and sealing and the like on samples such as blood, cells and microorganisms and the like.

The invention discloses a brake pad double-sided wear resistance detection device, and further discloses a detection method of the brake pad double-sided wear resistance detection device. The device comprises a detection mechanism and a friction mechanism, and the two sides of the detection mechanism are movably connected with the friction mechanism; according to the device, after a rotating rod reaches the set rotating times, a servo motor is turned off and a lifting column is lifted; meanwhile, a gear motor is started to drive a rotating shaft to rotate an upper clamping frame to a certain angle; after the device is used for abrading the front surface of the brake pad, the gear motor rotates to drive the rotating shaft, the reverse side of the brake pad is convenient to face upwards forreverse side abrasion test; after an arc-shaped grinding block makes contact with the front surface of the brake pad, an air cylinder drives a push rod to reciprocate, the friction state of the brakepad under a real state is simulated, so that wear resistance test is carried out on the brake pad, double-sided wear resistance detection is conducted by means of the two test results, and compared with a traditional brake pad single-side wear resistance detection mode, the detection in the invention is more comprehensive, and the safety coefficient is higher after the brake pad is detected.

The invention relates to a visual rapid detection method for canine distemper viruses. According to the detection method, on the basis of fusion of an immunochromatography technique and molecular biology means and through combination of LAMP which is high in amplification efficiency and lateral flow dipstick which is convenient for result observation, a detection result is more intuitive; the detection method has the characteristics of being visual, rapid in diagnosis, accurate and the like; shortcomings of conventional detection methods which are complex in operation, low in sensitivity and low in specificity can be overcome; and the detection method provided by the invention is applicable to fields of clinical diagnosis, food safety, animal disease detection and the like.

The invention provides a primer group for identifying porcine circovirus types 1, 2 and 3 and application of the primer group. The sequences of the primer group are shown in SEQ ID NO.1 -4. On the basis of the genome structure characteristics of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus, genome specific conserved areas of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus are selected as upstream primer design areas, and downstream primers are designed separately in differential areas of genomes generated according to the three genotypes (PCV1, PCV2 and PCV3). The primer is simple in condition optimization, convenient and high in reaction speed, and a result is observed conveniently; an assembled kit can be used for rapid differential diagnosis of the three genotypes of porcine circovirus (PCV1, PCV2 and PCV3).

The invention discloses an RT-LAMP (reverse transcription loop-mediated isothermal amplification) primer set and kit for detecting porcine epidemic diarrhea virus and application thereof. The primer set is composed of a pair of outer primers, a pair of inner primers and a pair of loop primers. Sequences of the paired outer primers are shown in SEQ ID No.1 and SEQ ID No.2. Sequences of the paired inner primers are shown in SEQ ID No.3 and SEQ ID No.4. Sequences of the paired loop primers are shown in SEQ ID No.5 and SEQ ID No.6. The primer set is effective in detecting the porcine epidemic diarrhea virus. The kit comprises the afore-mentioned primers and is applicable to detecting the porcine epidemic diarrhea virus. The invention further provides a method of detecting the porcine epidemic diarrhea virus with the kit; detection results of the method may be visually detected according to changes in the color of reaction liquid, quickly and accurately. The primer set, the kit and the method are highly specific, highly sensitive and quick and accurate in detecting the porcine epidemic diarrhea virus and are particularly applicable to quick field detection.

The invention discloses a method for rapidly detecting pseudomonas aeruginosa in a textile. The method comprises designing a set of LAMP specific primers based on the pseudomonas aeruginosa specific ETA conserved sequence as target gene sequence, carrying out enrichment culture on a textile sample, carrying out product LAMP amplification to obtain white precipitates, comparing and observing the precipitates to determine a result or observing a result through a turbidity meter, wherein when the sample reaction turbidity value is less than 0.1, the sample result is negative and when the sample reaction turbidity value is greater than or equal to 0.1, the sample result is positive, separating the positive sample enrichment fluid inoculation flat plate, picking a typical or suspicious colony on the flat plate, and carrying out fast identification through a MALDI-TOF-MS technology to obtain an identification result. The method has the advantages of simple operation, fastness, high efficiency, strong specificity, high sensitivity and convenient and visual observation.

The invention discloses a trichomonad nested PCR detection kit and a preparation method. A primer is designed according to a part of conserved sequences of 16S rRNA genes of trichomonad and trichomonas hominis daraine, a specific nested PCR detection method is established, the sensitivity and accuracy of the detection method are improved by optimizing the concentration of the magnesium <2+> involved in PCR reaction, annealing temperature and other reaction conditions, and result is convenient and quick to observe after an amplification prdocut is subjected to 1.0% agarose gel electrophoresis. The trichomonad and the trichomonas hominis daraine can be rapidly detected only by using one nested PCR primer, and the trichomonad nested PCR detection kit has the advantages of being simple in operation, high in high sensitivity, good in specificity and the like.

The invention provides a diaper with a detection function. The diaper comprises a diaper cover and an absorber, wherein the diaper cover comprises a rear waist part, a crotch part and a front waist part in sequence, and the absorber is arranged on the inner side of the diaper cover; first bonding tapes are arranged at two ends of the bottom of the absorber, and corresponding second bonding tapes are arranged on the inner sides of two ends of the diaper cover; the absorber comprises a surface layer, a flow guide layer, an absorber core and a transparent breathable bottom film in sequence; a flow guide groove is formed in the absorber core and runs through the absorber core; detection test paper is arranged under the flow guide groove. According to the diaper with the detection function, the test paper and the diaper are combined, so that safe, convenient and noninvasive conventional detection can be realized.

The invention discloses a small device for blood type detection, which utilizes a micro direct current motor to drive a bar-shaped magnet fixed on a rotating shaft of a micro direct current motor to rotate, iron beads in a microwell plate are driven via magnetic force to rotate, so that reagents and cells in the microwell plate are fully and uniformly mixed, and micro-centrifugation effect is achieved. The device does not need to be connected with an alternating-current power supply, and can be used for detecting various blood type systems such as ABO, Rh and the like; the device is suitable for being used in environments without large instruments or power supplies, such as field operations, sudden public health events, mobile laboratories and the like. According to a blood type detectionmethod, plasma and red blood cells are rapidly separated by using a filter, blood type detection is carried out by using a microporous plate as a carrier, and antigen and antibody reactions are promoted through rotation of iron beads. The microwell plate can be capped and sealed; compared with an existing detection method from the aspect of biosafety, the blood type detection method is advantageous in that aerosol does not overflow in a detection and interpretation process, especially in some infectious disease epidemic periods; workers can be efficiently protected against harm of pathogenic microorganisms via the small device for blood type detection and the blood type detection method..

The invention discloses a meteoric trail communication modeling simulation system which relates to a meteoric trail burst communication system simulation apparatus in the field of communication. The meteoric trail communication modeling simulation system comprises parts: a channel simulator, a transmitter, a receiver, a data source generator, a counter and the like. The meteoric trail communication modeling simulation system has authenticity, can simulate year, month and day change laws of a meteoric trail, and can reflect a topological structure and an operation condition of an actual meteoric trail communication network; the meteoric trail communication modeling simulation system has expandability, and can simply and conveniently construct network forms with different scales and topological structures; and the meteoric trail communication modeling simulation system has maneuverability, is provided with an excellent human machine interface and is convenient to change simulation parameters and observe and display a result. According to the simulation system disclosed by the invention, by loading a link, an internetworking protocol and the like of the meteoric trail communication network, the entire communication process can be efficiently and rapidly simulated. The meteoric trail communication modeling simulation system is particularly suitable for evaluating performance of the meteoric trail communication link and network, a meteoric trail communication network protocol system is optimized, and basis is provided for building and use of the meteoric trail communication network.

The invention provides a laser processing and real-time in-situ high-resolution observation device of a micro-nano structure, which comprises a laser, a CCD (Charge Coupled Device) camera and a light source, the laser is focused on a sample through a laser processing light path, and the light source obliquely irradiates the sample through an illumination light path and is reflected into the CCD camera through a microscopic imaging light path; a laser beam sequentially passes through an electronic shutter, a gradual-change neutral optical filter, a long-focus lens, a reflecting mirror group, a dichroscope and a microobjective and is focused on a sample, so that a laser processing light path is formed; illumination light sequentially passes through the short-focus lens, the beam splitter, the dichroscope and the microscope objective and obliquely irradiates a sample to form an illumination light path; illuminating light reflected by the sample sequentially passes through the microscope objective, the dichroscope and the beam splitter and enters the CCD camera, and a microscopic imaging light path is formed. Short-wavelength illumination light is adopted on an illumination light path, the imaging quality is improved in a mode of illuminating a sample in an oblique incidence mode, and in-situ observation on the surface of a material in the machining process is achieved.

The invention discloses a high-precision dew-point instrument provided with a dual-optical-path system. The high-precision dew-point instrument comprises a temperature sensing unit, a dual-optical-path detection unit and an observing unit, wherein the temperature sensing unit comprises a temperature sensor, a sensor prefixed amplifying circuit connected with the temperature sensor, an A / D conversion circuit connected with the sensor prefixed amplifying circuit and a nonlinear correction control circuit, wherein a nonlinear correction control system divides an input signal into different sections according to different temperature values and then enables the sections where the input signal is located to multiply by different compensation factors to perform correction. The dual-optical-path detection unit comprises a reflected light measurement module and a scattered light measurement module, wherein the reflected light measurement module and the scattered light measurement module perform measurement simultaneously. The observing unit comprises an observing microscope for observing a mirror plane and facilitating accurate and timely result observing performed by a user. The high-precision dew-point instrument is high in measurement accuracy, more convenient to use and more accurate in measurement.

The invention discloses a cell lysis reagent containing a graphene oxide component. The reagent can achieve mild and complete lysis of cells by utilizing excellent properties of graphene oxide. The defect that a common mechanical lysis method for lysis of cells is not suitable for large-batch detection is overcome, and the defect that a cell lysis solution on the market is inconvenient to operateis overcome. According to the method, an isotonic solution cooperates a the buffer solution to replace unstable guanidine salt, and a graphene oxide solution is added into the reagent, so that the lysis process of sample cells can be accelerated under the condition that a sample is not obviously damaged, the sample treatment time is shortened, experimental treatment is facilitated, and the reliability of sample detection is guaranteed. The improved cell lysis reagent disclosed by the invention has the advantages of mild conditions, sufficient cell lysis and simplicity and convenience in operation.

The invention relates to a rapid PCR amplification and CRISPR visual detection device and method. The visual detection device comprises a closed module and a heating module, wherein the closed modulecomprises a lower carrier, an upper cover body, a plurality of PCR amplification chambers arranged in the lower carrier and a plurality of CRISPR reaction chambers of the corresponding number; the amplification chambers are communicated with the corresponding CRISPR reaction chambers through connecting channels; the upper cover body is connected with the lower carrier in a sealed manner and all the chambers and the connecting channels are enabled to be sealed; and the heating module is used for heating the PCR amplification chambers and / or the CRISPR reaction chambers. Compared with the priorart, the device has the advantages that the specific surface area is large, and the heat transfer efficiency is improved, so that the PCR amplification time is greatly shortened, and the total detection time is further shortened to be within a few minutes. According to the invention, amplification of a plurality of PCR systems and CRISPR visual detection can be realized, and the temperature-controllable heating module is integrated in the detection device, so that the detection is more convenient, and the detection time is shortened.

The invention discloses a double-PCR (polymerase chain reaction) detection kit and method for giardia and cryptosporidia of sheep. According to parts of conserved sequences of giardia duodenalis TPI genes and cryptosporidium parvum 18S rRNA genes, a primer is designed, a double-PCR detection method is built, sensitivity and accuracy of the method are improved by optimizing reaction conditions suchas annealing temperature of PCR reaction, amplification products are implemented by 1.0% of agarose gel electrophoresis, and a result is conveniently and rapidly observed. Distinguishing of fragmentswith different targets is one of keys of building the double-PCR detection method, two target fragments amplified by the method have size differences in agarose gel electrophoresis and are easily distinguished, the sensitivity can reach 1*10<3> / g and is higher than that of a reported microscopy method and a conventional PCR detection method of giardia duodenalis and cryptosporidium parvum, and the method is suitable for detection of samples in clinical practice. The detection method can simultaneously and rapidly detect the giardia duodenalis and the cryptosporidium parvum of the sheep and has the advantages of simplicity in operation, high sensitivity, high specificity and the like, and technical support is provided for quality standards of real experiment sheep.

The present invention relates to a composition comprising moxidectin or a salt thereof for use in preventing and / or treating a parasite infestation in non-human mammals, in which said composition is topically administered every 3 to 9 months.

The invention belongs to the technical field of electrical equipment, and particularly relates to a cable terminal electroscope which comprises an insulation handle. The insulation handle comprises a first handle section, a second handle section and a third handle section, wherein the first handle section, the second handle section and the third handle section are connected through a rotary shaft. The third handle section is connected with one end of a base. A display and an alarm are arranged on the base. The other end of the base is connected with a connection rod. The connection rod comprises a first connection rod and a second connection rod. The second connection rod is connected with a connection plate. The connection plate is connected with a verification straight peen and a verification elbow. The cable terminal electroscope provided by the invention has the advantages of wide application range and convenient use, and can ensure the personal safety of staff.

The presnet invention relates to a high-precision gravireceptor and relates to geoscience observation technigue, in particular, it is suitable for use in solid tide research and observation of preearthquake warning signs. It is composed of machine main body, thermostat, micrometer, and collector. We use vertical hanging elastic system, double layer thermostat and feedback temperature control and three piece type capacitance micrometer. Advantages are: simple structure, high sensitivity and high precision.