Quick identifying method and use for new low mycobacterium and tubercle mycobacterium

A technology of Mycobacterium tuberculosis and Mycobacterium bovis is applied in the field of rapid identification and application of new Mycobacterium bovis and Mycobacterium tuberculosis, which can solve the difficulty of identification and the time required for Mycobacterium bovis and Mycobacterium tuberculosis long and other problems, to achieve the effect of easy operation

Inactive Publication Date: 2006-01-04
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
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Problems solved by technology

[0005] Aiming at the disadvantages of long time and difficulty in identifying Mycobacterium bovis and Mycobacterium tuberculosis, the invention solves the problem of rapid identification of Mycobacterium bovis and Mycobacterium tuberculosis

Method used

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Examples

Experimental program
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Embodiment 1

[0021] PncA (pyrazinamidase) gene is a unique gene that exists in Mycobacterium bovis and Mycobacterium tuberculosis, has a high degree of homology, and homologous sequence analysis shows that Mycobacterium bovis and Mycobacterium tuberculosis PncA ( pyrazinamidase) genes. Design the same upstream primers and downstream primers containing differential genes, through the difference in the binding force between the downstream primers and the DNA template and the control of high annealing temperature, to specifically identify Mycobacterium bovis and Mycobacterium tuberculosis; The gene does not exist in Bacillus, and thus allows the distinction between M. bovis, M. tuberculosis and atypical mycobacteria.

[0022]1. The upstream primer MB-F and the downstream primer TB-R of Mycobacterium bovis respectively amplify the genomic DNA of Mycobacterium bovis Vallee strain, Mycobacterium tuberculosis H37Rv strain, Mycobacterium paratuberculosis bovis and Mycobacterium phlei.

[0023] Sp...

Embodiment 2

[0033] Application of a rapid method for differentiating Mycobacterium bovis from Mycobacterium tuberculosis in direct detection of bacterial pure cultures and clinical samples.

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Abstract

The quick method of identifying bovine mycobacterium and tubercle mycobacterium includes: adding bovine mycobacterium Vallee strain genome DNA to proliferate one 294 bp segment, detecting target gene from bovine mycobacterium DNA with bovine mycobacterium primer, adding tubercle mycobacterium H37vR strain genome DNA to proliferate one 294 bp segment, proliferating target genome from tubercle mycobacterium DNA with tubercle mycobacterium primer, adopting the same upstream primer and different downstream primers and adopting two gene segments of 294 bp and annealing temperature of 67 deg c. The method of present invention is used in the direct detection of bacterial cultured matter and clinical sample.

Description

Technical field: [0001] The invention relates to a novel and rapid identification method of mycobacterium bovis and mycobacterium tuberculosis. Background technique: [0002] Tuberculosis is a chronic infectious disease shared by humans and various animals. The pathogenic bacteria of this disease is Mycobacterium tuberculosis. and Mycobacterium avium tuberculosis (or avian type) are three types of tuberculosis bacilli that are pathogenic to humans and many animals. Mycobacterium bovis can not only make cattle sick, but also infect humans and various animals; besides causing human tuberculosis, human tuberculosis is also dangerous to animals such as monkeys, cows, pigs, and cats; the same is true for avian tubercle bacillus. It can cause some animals other than poultry to suffer from tuberculosis. As early as 1947, the first human avian tuberculosis was discovered in the United States. So far, in addition to humans, at least 50 species of mammals such as cattle, horses, she...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 刘思国王春来王牟平宫强彭永刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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