Pure honeysuckle polymerase chain reaction-restriction map identification method
A technology of restriction endonuclease and honeysuckle, which is applied in the field of quality identification of Chinese medicinal material germplasm, can solve the problems such as inability to distinguish honeysuckle in shape and microscopic pattern, achieve a reliable identification method and ensure the quality of medicinal materials.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0014] a. DNA extraction: proceed as usual, and adjust the DNA concentration of the test sample to 0.1-0.3 μg / μl with deionized water;
[0015] a. Polymerase chain reaction:
[0016] (1) For polymerase chain reaction, 30 μl is used as a reference, and the dosages of various items are as follows:
[0017] 10×PCR buffer 3μl
[0018] MgCl 2 (25mM) 2.4μl
[0019] dNTP (each 10mM) 0.6μl
[0020] Primers LjP1, LjP2 (30pmol / μl) 0.5μl each
[0021] 1~2 μl DNA template for test product
[0022] TaqDNA polymerase 1U (0.2μl)
[0023] Deionized water make up to 30μl
[0024] After mixing, centrifuge at high speed for a few seconds
[0025] (2) PCR reaction conditions: the reaction is carried out on a PCR instrument, and the reaction conditions are:
[0026] Pre-denature at 97°C for 4 minutes, and then carry out 30 cycles according to the following conditions:
[0027] Denaturation at 97°C for 15 seconds
[0028] Refolding at 53°C for ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com