The invention discloses a short-tandem-repeats (STR) primer combination for source-traced identification on a beef product and a detection kit. The invention provides a primer kit for identifying beef. The primer kit is (1) or (2), wherein the (1) consists of primer pairs BM1818, ILSTS005, INRA032, INRA035, BM1824, HEL5, HEL13, ILSTS006, CSRM60, INRA005, INRA037, INRA063, ETH10, ETH225, NRA023 and TGLA1225; the (2) consists of a primer group A, a primer group B, a primer group C and a primer group D; the primer group A consists of primer pairs BM1818, ILSTS005, INRA032 and INRA035. According to the method provided by the invention, the selected 16 STR markers have the advantages that the polymorphism is high, the markers are not in linkage, amplified fragments have polymorphism, and genotyping is facilitated; primer sequences of different marker bits are grouped and are modified by using different fluorescent dyes, so that grouped multiplex PCR (Polymerase Chain Reaction) detection can be achieved, the cost is reduced, the time is shortened, and the efficiency is increased.