SSR (simple sequence repeat) molecular marker for detecting fertility of stamens of beta vulgaris and application of SSR molecular marker

A molecular marker and stamen technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of lack of molecular markers, difficult to identify the fertility of sugar beet stamens, etc., and achieve good stability and shorten the identification. Accurate effect of time and identification method

Active Publication Date: 2017-10-20
HEILONGJIANG UNIV +1
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

[0007] The present invention aims to solve the problem of the lack of existing molecular markers related to the stamen fertility of sugar beet, and the problem that it is not easy to identify the stamen fertility of sugar beet, and provides a SSR molecular marker for detecting the fertility of sugar beet stamens and its application

Method used

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  • SSR (simple sequence repeat) molecular marker for detecting fertility of stamens of beta vulgaris and application of SSR molecular marker
  • SSR (simple sequence repeat) molecular marker for detecting fertility of stamens of beta vulgaris and application of SSR molecular marker
  • SSR (simple sequence repeat) molecular marker for detecting fertility of stamens of beta vulgaris and application of SSR molecular marker

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specific Embodiment approach 1

[0027] Specific embodiment one: present embodiment detects the SSR molecular marker of beet stamen fertility, and the primer pair that is used for PCR amplification this molecular marker is BvRE051-S and BvRE051-A, and specific sequence is:

[0028] BvRE051-S: 5'-TCCAACTTTGGGACTCTAAATTAAC-3'

[0029] BvRE051-A: 5'-GAAAGTGACGGCGAGAATGT-3'.

[0030] In this embodiment, the SSR molecular markers related to the stamen fertility of sugar beet are obtained, and used alone or in combination with other primers, the stamen fertility of sugar beet can be pre-judged by PCR detection, and genomic DNA can be extracted as a material at any growth stage of sugar beet , reduce the workload and shorten the cycle of fertility judgment.

[0031] Markers can be used to judge the fertility of germplasm or individuals. When used to judge the fertility of the entire germplasm, it can be judged directly based on the PCR results combined with statistical analysis, or the trait investigation of key i...

specific Embodiment approach 2

[0033] Embodiment 2: The application of the SSR molecular marker for detecting the stamen fertility of sugar beet in this embodiment is used to identify the stamen fertility of sugar beet.

specific Embodiment approach 3

[0034] Specific implementation mode three: the difference between this implementation mode and specific implementation mode two is: the specific identification method is:

[0035] 1. Separation and extraction of total DNA from young sugar beet leaves;

[0036] 2. Using the DNA obtained in step 1 as a template, use primers BvRE051-S and BvRE051-A to carry out PCR amplification,

[0037] 3. The PCR amplification product obtained in step 2 is separated by polyacrylamide gel electrophoresis, and the result is judged according to the size of the amplification product. If only a 172bp band is detected, it is judged as stamen sterility; In addition to the bands of 232bp and 274bp, it was determined that the stamens were fertile. Others are the same as in the second embodiment.

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Abstract

The invention relates to an SSR (simple sequence repeat) molecular marker for detecting fertility of stamens of beta vulgaris and application of the SSR molecular marker, and can be applied to detecting fertility of stamens of beta vulgaris. By the aid of the SSR molecular marker and the application, the problems of deficiency of molecular markers related to fertility of stamens of beta vulgaris and difficulty in identifying the fertility of the stamens of the beta vulgaris can be solved. Primer pairs for carrying out PCR (polymerase chain reaction) amplification on the molecular marker are BvRE051-S and BvRE051-A. The SSR molecular marker can be used for identifying the fertility of the stamens of the beta vulgaris. The SSR molecular marker and the application have the advantages that the consistency of detection results obtained by the aid of the SSR molecular marker and the performance of traits of the fertility of the stamens of the beta vulgaris can reach 70% and can reach 63.33% and 76.67% in fertile and infertile individuals; chain relations between the SSR molecular marker and the fertility of the stamens are shown, and accordingly the SSR molecular marker can be used for detecting the fertility of the stamens.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and relates to an SSR molecular marker for detecting beet stamen fertility and an application thereof. Background technique [0002] Sugar beet (Beta Vulgaris) has dual pollination pathways of wind vector and insect vector, and hybridization is very easy to occur. Therefore, it is very important to identify the fertility of stamens and obtain male sterile germplasm for the hybrid breeding of sugar beet. The male sterile line is used as the female parent The hybridization can ensure the accuracy of the hybridization result, save labor and improve the success rate. However, the acquisition of sugar beet male sterile lines and corresponding maintainer lines is time-consuming and laborious, and germplasm resources are scarce. Male sterile materials in my country mainly come from five ways: introduction from abroad, natural mutation male sterile plants, separation of hybrid offspring, chemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王希陈丽赵春雷徐杰
Owner HEILONGJIANG UNIV
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