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Detecting and identifying method for germ plasm of blue crab

An identification method, the technology of blue crab, is applied in the germplasm detection and identification of blue crab, and the field of germplasm detection and identification of blue crab along the coast of China. reliable results

Inactive Publication Date: 2007-01-24
OCEAN UNIV OF CHINA
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Problems solved by technology

[0003] At present, the blue crab breeding industry is still in the initial stage, and the status of blue crab resources is grim

Method used

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  • Detecting and identifying method for germ plasm of blue crab

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Embodiment Construction

[0022] Below in conjunction with specific embodiment, further set forth the present invention;

[0023] Experimental material of the present invention:

[0024] In the sample of the present invention, the collection situation of 3 kinds of crabs and the blue crab sample along the coast of China is as follows: S.serrata is collected from Japan's Wakayama Prefecture Kinokawa, Japan's Kochi, Japan's Shizuoka Hamana Lake, Japan's Okinawa and Madagascar; .olivacea was collected from Kinogawa, Wakayama Prefecture, Japan, Lake Hamana, Shizuoka, Japan, Okinawa, Japan, and Surat Thani, Thailand; S.paramamosain was collected from Kinogawa, Wakayama Prefecture, Japan, Kochi, Japan and Vietnam; mud crab samples from the coast of China (temporarily Named: Scylla spp.) collected from Fujian, Wenzhou and Hainan in China. After alcohol fixation or DNA samples were transported to the Fishery Resources Laboratory of Ocean University of China, they were stored below -20°C for later use. In the...

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Abstract

The blue crab germ plasm detecting and identifying method includes: 1. extracting DNA of blue crab sample; 2. PCR amplification and purification with the extracted DNA as template; 3. measuring the DNA sequence of PCR amplifying product; and 4. analyzing the sequencing data through selecting standard comparison sequence, comparing and identifying haplotype sequence length, haplotype distribution, base A, T, G and C component persentages, haplotype number, interspecies net genetic distance and individual genetic diversity, and constituting system cladogram. Chinese blue crab is detected to be S.paramamosain. The present invention is effective molecular marking technology.

Description

technical field [0001] The invention relates to the improvement of the inspection technology of marine economic aquatic crabs, in particular to a germplasm detection and identification method of blue crabs, which belongs to the technical field of germplasm detection and identification of blue crabs along the coast of China. Background technique [0002] Crabs of the genus Scylla belong to Crustacea, Decapoda, and Portunidae. They are widely distributed in mangrove areas and estuaries in temperate, subtropical, and tropical seas of the Pacific Ocean and Indian Ocean. An important component of coastal fisheries in many developing countries. Blue crabs are all produced in the south of the Yangtze River in my country, especially in Guangdong, Guangxi, Hainan, and Fujian. They are one of the important marine economic crabs in my country. In recent years, many domestic scientific research workers have devoted themselves to the artificial seed cultivation and breeding of blue crab...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 高天翔王玉江陈艳翠程光平
Owner OCEAN UNIV OF CHINA
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