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Method for identifying seeds of glycyrrhiza uralensis and glycyrrhiza inflata batalin

A technology of Ural licorice and bulging licorice, applied in the field of plant identification, can solve the problems of indistinguishability, difficulty, loss, etc.

Inactive Publication Date: 2013-09-11
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, it is very difficult for people to distinguish the seeds of Ural licorice and Glycyrrhiza inflata, because the seeds and seedlings of the two are very similar. Only after two or three years of planting can they be distinguished from the leaves, fruits, ears and roots that are more than two years old. , but the distinction is meaningless at this time, because irreparable losses have been caused

Method used

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  • Method for identifying seeds of glycyrrhiza uralensis and glycyrrhiza inflata batalin
  • Method for identifying seeds of glycyrrhiza uralensis and glycyrrhiza inflata batalin

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Embodiment 1

[0059] (1) Preparation of enzyme solution: take the licorice seeds to be identified, soak them in tap water for 6 hours, place them in a container at room temperature, keep them moist and allow them to germinate (place the seeds in a petri dish covered with soaked filter paper, Cover the lid and add water twice a day to keep the filter paper moist. When the radicle grows to 1-1.5cm (about 6 days), add 1ml electrode buffer to 1g sample and grind it at 0-4°C. Centrifuge at 10,000 rpm for 10 minutes, the supernatant is the SOD extract; the electrode buffer refers to the electrode buffer with pH 8.3.

[0060] (2) Gel-making plate: wash the glass plate, put the sealing strip, put it into the electrophoresis tank, insert the wedge; then fill the glue: fill the separation glue first, seal it with water, and absorb the water seal when the boundary appears , Pour the concentrated gel and insert the comb. After the concentrated gel has solidified, remove the comb, remove the sealing strip,...

Embodiment 2

[0077] 1. Preparation of enzyme solution: take the licorice seeds (20 tablets) that are hard to be identified, soak them in concentrated sulfuric acid for 30 minutes, then rinse the surface of sulfuric acid with water, soak in tap water for 8 hours, and place them in a container at room temperature In the middle, keep it moist to make it germinate (put the seeds in a petri dish covered with soaked filter paper, cover the dish, add water twice a day to keep the filter paper moist), wait until the radicle grows to 1.0-1.5cm ( About 6 days), add 1ml electrode buffer solution to 1g sample, and grind it at 0-4℃, 10,000 rpm, centrifugation for 10 minutes, the supernatant is the SOD extract.

[0078] 2. Preparation of polyacrylamide gel

[0079] (1) Preparation of gel mother liquor:

[0080] 30% acrylamide: Take 29.2g of acrylamide and 0.8g of methylene bisacrylamide, dissolve in deionized water, and dilute to 100ml.

[0081] 1.5mol / L Tris-HCl buffer (pH 8.8): Take 18.17g of Tris, ...

Embodiment 3

[0101] Actual usage

[0102] In March 2012, we bought licorice seeds (purchased according to Ural licorice seeds) from farmers in Xinjiang and prepared to use them in the experiment. Since the seller did not know whether it was Ural licorice or inflated licorice seeds, we used our identification method for identification. , The identification result of the SOD isoenzyme spectrum is the same as the two lanes on the right side of the standard zymogram, which proves that the seeds purchased are Glycyrrhiza inflata seeds. We also sent photos of Glycyrrhiza inflata and Ural licorice fruit to the seller for confirmation, and the seller confirmed that the licorice fruit they picked was Glycyrrhiza inflated. This fully proves that our method of identifying the seeds of Glycyrrhiza Uralensis and Glycyrrhiza inflata is reliable.

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Abstract

The invention discloses a method for identifying the seeds of glycyrrhiza uralensis and glycyrrhiza inflata batalin, which comprises the following steps of: preparation of enzyme liquid, preparation of a gel plate, sample application and electrophoresis, SOD (superoxide dismutase) isozyme dyeing process, variety conformation and the like. The method mainly makes use of the physiological and biochemical difference between the seeds of glycyrrhiza uralensis and glycyrrhiza inflata batalin, and takes the SOD isozyme zymogram as an index for identifying the seeds of glycyrrhiza uralensis and glycyrrhiza inflata batalin. If the SOD isozyme zymogram of the seed to be detected is the same as the two lanes on the left side of a standard zymogram, the seed is the glycyrrhiza uralensis seed; if the SOD isozyme zymogram of the seed is the same as the two lanes on the right side of the standard zymogram, the seed is the glycyrrhiza inflata batalin seed. The identifying method disclosed by the invention has the advantage of simple, quick and accurate identification.

Description

technical field [0001] The invention belongs to the technical field of plant identification, and relates to an identification method for licorice uralensis and licorice bulging seeds. Background technique [0002] Artificially grown licorice, including ural licorice, licorice glabra and licorice inflate. Among them, Ural licorice is the most popular, because among the three kinds of licorice allowed to be used as medicine in Chinese Pharmacopoeia, Ural licorice has the best quality and the highest content of glycyrrhizic acid (research progress on quality evaluation methods of licorice, Chen Yunhua, Wang Wen, Quanshizhen Guoyi Guoyao, 2008, 19 ( 6): pp. 1054-1055). In accordance with the relevant requirements of the China Chamber of Commerce for Import and Export of Medicines and Health Products "Notice on Printing and Distributing Prices of Licorice and Licorice Products Export Industry in the Second Half of 2010" (Yishangzhaozi [2010] No. 2), licorice will be adjusted fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 王景安高一波梁晓华
Owner TIANJIN NORMAL UNIVERSITY
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