Visual nucleic acid detection method for canine distemper viruses

A technology of canine distemper virus and detection method, which is applied in the field of visual and rapid detection of canine distemper virus, can solve the problems of cumbersome operation and low specificity, and achieve the effect of solving cumbersome operation

Inactive Publication Date: 2018-09-28
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the defects of cumbersome operation, low sensitivity and low specificity of traditional detection methods, and provides a visual rapid detection method for canine distemper virus, which can detect canine distemper virus conveniently and quickly

Method used

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  • Visual nucleic acid detection method for canine distemper viruses
  • Visual nucleic acid detection method for canine distemper viruses
  • Visual nucleic acid detection method for canine distemper viruses

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Experimental program
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Embodiment 1

[0030] A method for detecting canine distemper virus

[0031] 1. Materials and methods

[0032] 1.1 Virus strains

[0033] Canine distemper virus (vaccine strain Onderstepoort), canine parvovirus (CPV), canine adenovirus (CAV), canine parainfluenza virus (CPIV), canine distemper 10th generation strain; Vero cells; CDV plasmids all provided by military veterinarians Stored in the virus room of the research institute;

[0034] 1.2 Main reagents

[0035] Bst DNA polymerase, MgSO 4 , 10×Amplification buffer, and AMV enzyme were purchased from NEB Company; dNTP was purchased from Solarbio Company; betaine was purchased from Sigma Company; viral genome DNA / RNA extraction kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; disposable nucleic acid The detection device (No. 3) was purchased from Hangzhou Ustar Biotechnology Co., Ltd.;

[0036] 1.3 Primer design

[0037] According to the CDV gene sequence included in GenBank, DNAM software was used for compa...

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Abstract

The invention relates to a visual rapid detection method for canine distemper viruses. According to the detection method, on the basis of fusion of an immunochromatography technique and molecular biology means and through combination of LAMP which is high in amplification efficiency and lateral flow dipstick which is convenient for result observation, a detection result is more intuitive; the detection method has the characteristics of being visual, rapid in diagnosis, accurate and the like; shortcomings of conventional detection methods which are complex in operation, low in sensitivity and low in specificity can be overcome; and the detection method provided by the invention is applicable to fields of clinical diagnosis, food safety, animal disease detection and the like.

Description

technical field [0001] The invention relates to a visual rapid detection method for canine distemper virus, which is used for the clinical diagnosis of canine distemper in animals and belongs to the technical field of animal epidemic detection. Background technique [0002] Canine distemper is an acute and highly contagious disease caused by Canine Distemper Virus (CDV). It has a wide range of susceptible hosts and can be cross-infected between different species. The disease is highly infectious and has a high incidence. The mortality rate of dogs and cats is as high as 30-80%. It is an important epidemic disease that endangers the health of animals, especially pet dogs and economic animals (mink, fox, and raccoon dog). Therefore, to fundamentally control and eliminate canine distemper disease, it is necessary to implement comprehensive prevention and control measures for dogs and cats. At present, the most important method for the prevention and control of canine distemper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12R1/93
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2521/107
Inventor 王承宇李筱鑫李吉平李楠郝镯万忠海孟轲音李忠义田多
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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