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A kind of chromogenic catalase reaction liquid and its application

A technology of reaction solution and catalase, which is applied in the field of color-changing catalase reaction reagents, can solve the problems of affecting the interpretation of results, the threat of aerosol personal safety, and the rapid disappearance of bubbles, etc., to improve accuracy and sensitivity, facilitate naked eye observation, and biological The effect of improving safety performance

Active Publication Date: 2016-04-06
ZHUHAI DL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional catalase reaction has certain limitations, mainly as follows: 1. The sensitivity is not high, and the generation of bubbles is less; 2. The generated bubbles disappear quickly, which affects the interpretation of the results and is often misjudged; 3. The generated bubbles are colorless Transparent, which puts forward higher requirements on the experience of clinical operators, and the subjective factors are relatively large; 4. The gas volume is too fast, and the aerosol poses a great threat to the personal safety of the operator; It expires in a week and needs to be reconstituted

Method used

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  • A kind of chromogenic catalase reaction liquid and its application
  • A kind of chromogenic catalase reaction liquid and its application
  • A kind of chromogenic catalase reaction liquid and its application

Examples

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Effect test

Embodiment 1

[0030] A chromogenic catalase reaction solution, the components of which are: 3.5% hydrogen peroxide, 10% oleyl dihydroxyethylamine oxide, 0.005% bromochlorophenol blue, and water as a solvent.

Embodiment 2

[0032] A chromogenic catalase reaction solution, the components of which are: 3% hydrogen peroxide, 0.008% sodium pyrophosphate, 15% oleyl dihydroxyethylamine oxide, 0.005% EDTA, 0.005% bromochlorophenol blue, solvent for water.

Embodiment 3

[0034] A chromogenic catalase reaction solution, the components of which are: 3.5% hydrogen peroxide, 0.008% sodium pyrophosphate, 15% oleyl dihydroxyethylamine oxide, 0.005% EDTA, 0.005% methylene blue, and water as a solvent.

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Abstract

The invention discloses a color-developing catalase reaction solution, which contains the following components: hydrogen peroxide, bubble protector and color indicator. The color catalase reaction solution of the present invention has the following advantages: 1) high stability, and the product validity period is extended from one week to one year in the traditional time; 2) it can produce color reaction, which is more convenient for the naked eye than the traditional colorless bubbles The observation results improved the accuracy and sensitivity of the experiment; 3) The amount of bubbles generated by the reaction was large, and the bubbles were stored for a long time, which improved the detection sensitivity compared with traditional methods; 4) The biological safety performance was greatly improved.

Description

technical field [0001] The invention belongs to the field of detection reagents, and in particular relates to a color-changing catalase reaction reagent and an application thereof. Background technique [0002] There are many types of microorganisms in nature and the human body, and new microorganisms are constantly emerging, which has brought great difficulties and challenges to workers engaged in microbial detection, and the number of microorganisms transmitted through the air is also increasing, which poses a threat to clinical operators. Personal safety poses a great threat. How to quickly and accurately identify pathogenic microorganisms that infect humans, while reducing biosafety infections, and maximizing the personal safety of clinical operators. These two aspects are a very important topic for clinical microbiology researchers and diagnostic reagent manufacturers. [0003] Catalase, also known as catalase, is a bacterium with catalase, which can catalyze hydrogen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/30C12Q1/04C12Q1/14C12R1/445C12R1/46
Inventor 林华青蔡桂林
Owner ZHUHAI DL BIOTECH CO LTD
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