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Primer group for identifying porcine circovirus types 1, 2 and 3 and application of primer group

A circovirus and primer set technology, applied in the field of animal infectious disease, can solve the problems of difficult to achieve research purposes, high possibility of crossover, complicated and complicated optimization of primer conditions, etc., and achieve rapid differential diagnosis, convenient observation, and simple optimization of conditions. Effect

Inactive Publication Date: 2019-05-17
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

However, the condition optimization of the six primers is cumbersome and complicated, and the more primers, the greater the possibility of potential crossover, making it difficult to achieve the relevant research purposes

Method used

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  • Primer group for identifying porcine circovirus types 1, 2 and 3 and application of primer group
  • Primer group for identifying porcine circovirus types 1, 2 and 3 and application of primer group
  • Primer group for identifying porcine circovirus types 1, 2 and 3 and application of primer group

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Embodiment 1

[0028] 1. Virus strain:

[0029] Porcine circovirus type 1 (PCV1) and porcine circovirus type 2 (PCV2), porcine circovirus type 3 (PCV3), porcine parvovirus (PPV), classical porcine pseudorabies virus (PRV), mutant porcine pseudorabies Rabies virus (V-PRV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine epidemic diarrhea virus (PEDV) were all isolated, identified and preserved by the Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences.

[0030] 2. Main reagents

[0031] EasyPure Viral DNA / RNA Kit was purchased from Beijing Quanshijin Biotechnology Co., Ltd.; PCR amplification kit (Quick Taq HS DyeMix) was purchased from TOYOBO.

[0032] 3. Nucleotide sequence analysis

[0033] Select representative PCV1 strain (PK strain, GenBank accession number DG650650; BJ-1 strain, GenBank accession number FJ475129) PCV2 representative strain (JZ-9 strain, GenBank accession number MH059556; PCV-45 strain, GenBank accession number ...

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Abstract

The invention provides a primer group for identifying porcine circovirus types 1, 2 and 3 and application of the primer group. The sequences of the primer group are shown in SEQ ID NO.1 -4. On the basis of the genome structure characteristics of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus, genome specific conserved areas of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus are selected as upstream primer design areas, and downstream primers are designed separately in differential areas of genomes generated according to the three genotypes (PCV1, PCV2 and PCV3). The primer is simple in condition optimization, convenient and high in reaction speed, and a result is observed conveniently; an assembled kit can be used for rapid differential diagnosis of the three genotypes of porcine circovirus (PCV1, PCV2 and PCV3).

Description

Technical field [0001] The invention belongs to the field of zoology, and specifically relates to a primer set for identifying porcine circovirus types 1, 2, and 3 and its application. Background technique [0002] Porcine circovirus (PCV) is one of the smallest animal viruses found so far. There are currently three types of porcine circovirus: PCV1, PCV2 and PCV3. PCV1 is a non-pathogenic virus; PCV2 is a pathogenic virus; the pathogenicity of PCV3 needs to be further clarified. Studies have found that PCV2 is the main pathogen of Postweaning Multisystemic Wasting Syndrome (PMWS), dermatitis and nephrotic syndrome (PNDS) in weaned piglets. [0003] Analysis of the genomes of PCV1 and PCV2 revealed that the full length of the PCV1 genome is 1759 bp, and the full length of PCV2 is 1768 bp (or 1767 bp). ORF1 is the largest open reading frame (ORF) of PCV1 and PCV2. It is located at the 5'end of the viral genome and encodes the viral replication protein (Rep) with a molecular weight...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
Inventor 陈如敬陈秋勇吴学敏王晨燕周伦江严山车勇良王隆柏魏宏刘玉涛
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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