Cell lysis reagent containing graphene oxide

Abstract

The invention discloses a cell lysis reagent containing a graphene oxide component. The reagent can achieve mild and complete lysis of cells by utilizing excellent properties of graphene oxide. The defect that a common mechanical lysis method for lysis of cells is not suitable for large-batch detection is overcome, and the defect that a cell lysis solution on the market is inconvenient to operateis overcome. According to the method, an isotonic solution cooperates a the buffer solution to replace unstable guanidine salt, and a graphene oxide solution is added into the reagent, so that the lysis process of sample cells can be accelerated under the condition that a sample is not obviously damaged, the sample treatment time is shortened, experimental treatment is facilitated, and the reliability of sample detection is guaranteed. The improved cell lysis reagent disclosed by the invention has the advantages of mild conditions, sufficient cell lysis and simplicity and convenience in operation.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a cell lysis reagent containing graphene oxide. Background technique [0002] Since the 1950s, molecular biology has been at the forefront and growing point of biology. With the continuous improvement of molecular biology technology, it has raised the research of the whole life science to a new stage. The main research field of molecular biology is the genetic factors in cells, which is what we call nucleic acids. In cells, nucleic acids are always combined with various proteins, and the most basic operation in molecular biology is to extract nucleic acids from cells. The extraction of nucleic acid mainly refers to the use of certain methods to lyse the cells of biological samples and release the nucleic acid from the cells. [0003] Commonly used lysis methods include chemical lysis, enzymatic lysis and mechanical lysis. Chemical and enzymatic cleavages are ...

AI Technical Summary

Problems solved by technology

In practice, due to the effect of guanidinium salts on lowering the pH value of the solution, the guanidinium salts in these cell lysates tend to precipitate when the pH value is close to neutral or slightly acidic, which not only brings about the need for heating and dissolving reagents, but also makes the pipetting operation difficult. Insufficient convenience, and it affects the cell lysis effect in the sample and reduces the yield of nucleic acid extraction