8 results about "Giardia" patented technology

Methods, devices, kits and compositions for detecting the presence or absence of one or more tapeworm coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of tapeworm in a fecal sample from a mammal and may also be able to distinguish between different tapeworm species and in the presence of one or more infections with helminths (such as roundworm, hookworm, whipworm and heartworm), Giardia and parvovirus. Confirmation of the presence or absence of tapeworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

The invention is suitable for the technical field of biology, and provides a giardia genome SNP bar code typing method based on machine learning optimization. According to the method, typing is completed through data acquisition and preprocessing, unsupervised group structure clustering, machine learning feature screening, bar code SNP panel construction, robustness evaluation and detection and interpretation. According to the method, an XGBoost model is trained by taking an unsupervised clustering result as a label, and 50 loci are screened in combination with an SHAP index to form an SNP bar code panel. The resolution ratio of the panel is close to that of whole genome SNP typing only by using a small number of sites, the performance of the panel is superior to that of MLST, and high accuracy is kept under low-quality data such as 15X low depth. Detection modes such as multiple PCR and the like and standardized reports are supported, the process adopts modular design and can be realized in a computer readable storage medium, and an effective tool is provided for giardia public health monitoring and cross-host traceability.

This application belongs to the field of in vitro detection technology, specifically relating to a fluorescent immunochromatographic kit for detecting canine parvovirus, canine coronavirus, and Giardia virus, as well as its preparation method and application. This application provides a fluorescent immunochromatographic kit for detecting canine parvovirus, canine coronavirus, and Giardia virus. Through a triple detection system design, it can simultaneously detect three important pathogens: canine parvovirus, canine coronavirus, and Giardia virus, improving detection efficiency and providing more comprehensive information for clinical diagnosis. Furthermore, by adding metal chelating agents and lysis agents to the sample diluent, metal ion impurities in the sample that cause non-specific binding can be specifically chelated, effectively eliminating interference and significantly improving the detection rate of the target pathogens while enhancing detection sensitivity, thus meeting the needs of rapid on-site screening in canine clinics.

The invention discloses a method for extracting giardia and cryptosporidium nucleic acid and application thereof. The method comprises the following steps: mixing a sample containing giardia and / or cryptosporidium with lysozyme and protease K, and incubating and digesting; adding a lysis enhancement solution and glass beads into the digested sample, carrying out ultrasonic treatment, and centrifuging after ultrasonic treatment to obtain a supernatant; and carrying out automatic nucleic acid extraction and purification on the obtained supernate. According to the method, the sample treatment time is shortened to be within 3 h, the detection efficiency is greatly improved, the detection cost is reduced, the cracking effect is excellent, the sensitivity is high, and the specificity is high.

The application relates to a monoclonal antibody of duodenum giardia CWP2 protein, a paired monoclonal antibody and application. The application obtains five pairs of paired monoclonal antibodies with better immunization effects through immunoscreening of duodenum giardia CWP2 protein, wherein the immunization effect is best when YC301 is used as a capture antibody and YC201 is used as a detection antibody, and a double-antibody sandwich ELISA detection method established by using the paired antibodies is better in sensitivity, specificity and stability, thereby laying a solid foundation for development of a duodenum giardia CWP2 antigen detection kit.

The invention discloses a primer group for simultaneously detecting four types of horse alimentary canal parasites and application of the primer group. The primer group comprises primer sequences as shown in SEQ.ID.NO.1-8, and the four types of horse alimentary canal parasites are ascaris horse parasite, strongylus arvensis, giardia blue and eimeria. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

Provided is a method for simultaneously detecting Giardia and Cryptosporidium in one tube. The nucleic acid sequence of a primer comprises sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 4, and the nucleic acid sequence of a fluorescent probe comprises sequences as shown in SEQ ID NO. 5 and SEQ ID NO. 6. The method achieves high-sensitivity specific amplification, allows for effective detection of Giardia and Cryptosporidium in drinking water currently supplied in China, and is easy to operate, efficient, cost-effective, and suitable for on-site rapid detection platforms.

The invention provides a magnetic bead solid-phase ELISA (enzyme-linked immunosorbent assay) kit for detecting giardia cysts and a preparation method thereof, the magnetic bead solid-phase ELISA kit is prepared by using an immunomagnetic bead process of biotin-streptavidin coupling reaction, the giardia cysts in faeces are enriched through immunomagnetic beads, and the concentration of the giardia cysts in the faeces is increased; the enriched magnetic bead-giardia capsule compound is added into an elisa plate coated with an antibody, so that an integrated process of magnetic bead enrichment, targeted adsorption and direct detection is realized.