56 results about "Giardia" patented technology

A method for the inactivation of Cryptosporidium oocysts, Giardia cysts and similar organisms comprising irradiating water with ultraviolet light in doses of from about 1 mJ / cm2 to about 175 mJ / cm2.

A method for stabilizing and removing Cryptosporidium oocysts or Giardia cysts from water. The method comprises adding chitosan, a salt or solution of chitosan to water containing Cryptosporidium oocysts or Giardia cysts and a halogenating agent. The method may also include adding a secondary polyelectrolyte flocculant to the water. The resulting flocs are filtered to remove the Cryptosporidium oocysts or Giardia cysts.

A method for stabilizing and removing Cryptosporidium oocysts or Giardia cysts from water. The method comprises adding chitosan, a salt or solution of chitosan to water containing Cryptosporidium oocysts or Giardia cysts and a halogenating agent. The method may also include adding a secondary polyelectrolyte flocculant to the water. The resulting flocs are filtered to remove the Cryptosporidium oocysts or Giardia cysts.

Raman scattering of radiation applied to a water sample is used to assess occurrence of a pathogen in the sample. The method is useful for detecting pathogens that are difficult to detect using other methods, such as protozoa. Examples of organisms that can be detected in water samples using these methods include protozoa of the genus Cryptosporidium and the genus Giardia. The methods described herein have important applications, such as for detection of Cryptosporidium organisms in municipal water systems.

The invention discloses a combined processing technology for slightly polluted raw water. The raw material water firstly enters into a first ozone contact tank for pre-ozonation, then enters into a flocculation sedimentation tank for sedimentation treatment, after sedimentation, the water enters into a secondary ozone contact tank for ozonation decomposition, then enters into a carbon sand filtering tank for filtering, the filtered water enters into a membrane processing system, the membrane-processed water enters into a clean water tank for dispatching and storing and are disinfected in the clean water tank by adding chlorine, and the water enters into a suction well and is conveyed to a water distribution network via a pump set in a clean water pump house. The combined processing technology of the invention has the advantages that: the finished water coming from the processed slightly polluted raw water can not only be guaranteed to reach the sanitary standard for drinking water, but also meet higher requirements in some indexes, and is eliminated in microbial overweight risk and effectively removed in humic acid and cryptosporidium and giardia. Additionally , compared with the prior art, the combined processing technology combined with advanced treatment process helps to saves land occupations of a sand filtering tank and a disinfection contact tank and enable water quality upgrading construction of an existing water factory with a compact field to be implemented.

Raman scattering of radiation applied to a water sample is used to assess occurrence of a pathogen in the sample. The method is useful for detecting pathogens that are difficult to detect using other methods, such as protozoa. Examples of organisms that can be detected in water samples using these methods include protozoa of the genus Cryptosporidium and the genus Giardia. The methods described herein have important applications, such as for detection of Cryptosporidium organisms in municipal water systems.

Raman scattering of radiation applied to a water sample is used to assess occurrence of a pathogen in the sample. The method is useful for detecting pathogens that are difficult to detect using other methods, such as protozoa. Examples of organisms that can be detected in water samples using these methods include protozoa of the genus Cryptosporidium and the genus Giardia. The methods described herein have important applications, such as for detection of Cryptosporidium organisms in municipal water systems.

The invention relates to a method and equipment for inactivating cryptosporidium and giardia cysts inside water. The method for inactivating the cryptosporidium and giardia cysts inside the water comprises the following steps: a first step: providing a water body to be processed; a second step: preparing a photocatalyst; and a third step: further adding the photocatalyst prepared from the second step into the water body obtained from the first step, and disinfecting the water body in a photocatalysis way under a lighting condition, wherein the amount of the photocatalyst is the same with the catalyst. The equipment comprises an ultraviolet light source irradiation device, a reactor used for accommodating the photocatalyst and the water body to be processed and a stirrer, wherein the stirrer is arranged in the reactor; and the ultraviolet light source irradiation device is arranged on the reactor. The method and the equipment have the advantages of high inactivating efficiency, low cost, easy operation, popularization and application, innocuity and no side product and can not cause secondary pollution.

The invention provides a formulation for treatment or prevention of a parasitic infection such as a protozoan or helminths infection, for example: Giardia. The formulation comprises at least one ganglioside, which may be selected from the group consisting of: GD3, GM1, GM2, GM3, GD1b, NANA, and sialic acid. The formulation may be used to supplement foods or liquids, for example: infant formula, baby food, baby cereal, juice, dehydrated camping food, or bottled water.

The invention discloses a sampling filtering bag for cryptospsridium and giardia detection in water and belongs to the field of water quality detection. The structure comprises a shell and filtering paper, wherein the filtering paper is arranged in the shell; a water inlet and a water outlet are formed in the two ends of the shell. The sampling filtering bag is characterized in that the shell is composed of an upper filtering cup and a lower filtering cup; the open end of the upper filtering cup is in butt joint with the open end of the lower filtering cup, and the open end of the upper filtering cup and the open end of the lower filtering cup are sealed by a sealing piece; the water inlet is formed in the top end of the upper filtering cup and the water outlet is formed in the bottom end of the lower filtering cup; the filtering paper is arranged between the open end of the upper filtering cup and the open end of the lower filtering cup. With the adoption of the sampling filtering bag disclosed by the invention, the detection cost can be greatly reduced and the detection recovery rate is improved; the sampling filtering bag has very good popularization and application values.

The invention relates to a membrane for detecting cattle and sheep Giardia in Haemaphysalis qinghaiensis and distinguishing the type. The membrane for detecting Theileria luwenshuni, Theileria uilenbergi and Giardia in cattle and sheep is prepared by fixing specific oligonucleotide of the Theileria luwenshuni and the Theileria uilenbergi or the specific oligonucleotide of Babesia and the universal specific oligonucleotide of Theileria on a Biodyne C membrane. The method for detecting comprises the steps: using DNA extracted from Haemaphysalis to be detected as a template; using a specific primer to perform PCR amplification; hybridizing the product with the oligonucleotide of the Theileria and Babesia for detecting; putting the hybridized membrane in an exposed template holder after enhanced chemiluminescence incubation; putting an X-ray film on the membrane for treatment of exposure, developing and the like; and determining whether the detected animal has the corresponding disease according to an intensity signal produced on the film.

The invention relates to a filtration and concentration method for improving the detection recovery rate of cryptosporidium and giardia in the technical field of water environmental water quality monitoring. The method comprises the following steps: sampling and filtering; adding elution; ultrasound oscillation and centrifugal treatment; carrying out stained preparation by sequentially adopting animmunomagnetic separation method after completing the centrifugal treatment; and then counting by a fluorescence microscope, and making statistics for the recovery rate. The method improves certain operation steps in the filtration and concentration stages, so as to achieve the purposes of reducing the detection cost of the cryptosporidium and giardia and improving the recovery rate.

The present invention relates to a giardia cyst in water and a cryptosporidium oocyst checking method which belonging to the detection technique field, the giardia and the cryptosporidium in water are detected by the combined-dyeing of haematein and intensified kinyoun after the filtration by microporous membrane and the gradient separation by acetic ether and formalin in a ratio of 10:1. The background complexity of the following microscopic examination is alleviated by membrane filtering the water sample and the giardia and the cryptosporidium are separately dyed by the combined-dyeing of haematein and intensified kinyoun. The invention alleviates the impurity interference in the background in traditional giardia and cryptosporidium detecting method and improves the microscopic examing confirmation accuracy of the morphological and structural features.

A continuous flow centrifuge adapted with an insert of glass beads, sand or the like is used to concentrate cysts of microorganisms such as giardia or cryptosporidium from large volumes of water. The apparatus and methods are useful for the detection of contaminating microoorganisms in groundwater and public drinking water.

The invention relates to a method for estimation of bacterial number in water by particle count in water, and the method belongs to the fields of drinking water processing and sanitation. First, for drinking water with turbidity lower than 1NTU, surface water in rivers and lakes, underground water, and industrial water with turbidity lower than 0.5NTU, a particle calculating instrument is used to determine particle count in certain particle size scopes, and determine bacteria number simultaneously; a certain amount of data is accumulated to satisfy statistical requirement after a plurality of detections, and function relationship between bacterial number and particle count in the certain particle size scope is established; and the function relationship is used to determine bacterial number in water from particle count in the certain particle size scope which is determined by the particle calculating instrument. The estimation method in the invention is simple and fast, can be used for on-line measurement, can determine conventional microorganism indexes such as the total number of bacterium colony, the total number of coliform group bacteria and the like, and can be used for determination of specific microbes such as cryptosporidium, giardia and the like.

The invention relates to the field of public drinking water hygienic security detection and provides a filtering recovery device related to cryptosporidium and giardia for solving the problem that theexisting device for detecting the cryptosporidium and giardia is complicated in detection programs and high in detection cost and a system thereof. The recovery device comprises a left filtering assembly, a right filtering assembly and a filter element, wherein the left filtering assembly comprises a water sample barrel, a water sample peristaltic pump, a left filter cup and a washing liquor collection barrel; a water sample inlet hole and a left filter cup exhaust hole are formed in the top of the left filter cup, and a washing liquor drain hole is formed at the bottom of the left filter cup; the right filtering assembly comprises a washing liquor barrel, a washing liquor peristaltic pump, a right filter cup and a water sample collection barrel; a washing liquor inlet hole and a right filter cup exhaust hole are formed in the top of the right filter cup, and a water sample collection hole is formed in the bottom of the right filter cup; solenoid valves are arranged at the previous holes; the filter element is fixedly arranged between the left filter cup and the right filter cup; the pore size of filter paper at the filter element is less than overall dimensions of the cryptosporidium and giardia.

The invention discloses a method for preparing a filter medium used for removing giardia and cryptosporidium in water. The method comprises the following steps: a) mixing raw materials containing ultra-high molecular weight polyethylene, activated carbon and diatomite in powder, wherein the weight ratio of the ultra-high molecular weight polyethylene, the activated carbon and the diatomite in powder is 100-300:50-100:100-350; and b) pressing, sintering and cooling mixture obtained in the step a) in a mould. Compared with the prior art, the invention has the advantages that the provided technical scheme can remove the giardia and the cryptosporidium in the water, has the removal rate reaching over 99.5 percent so as to achieve the aim of improving the water quality, and has simple method, low cost and quick response.

An efficient cyclic process and related compositions for the in-situ generation of oxyhalogens from anions of chloride, bromide and chlorite in an aqueous system using in-situ generated sulfate free radicals. The cyclic process and compositions enhance the rate of inactivation of microbiological organisms especially those resistant to inactivation from free halogen based sanitizers, and oxidation of oxidation resistant organic based compounds in aqueous solution. Aquatic facilities susceptible to accumulation of organic N-chloramines and other oxidation resistant compounds, as well as oxidation resistant parasitic organisms such as cryptosporidium and Giardia, obtain dramatic improvements in the rate of oxidation and subsequent inactivation of these undesirable contaminants.

The invention provides an immunofluorecence technique based method for evaluating activities of cryptosporidium parvum and giardia. The method has the following beneficial effects: the accurate, quick, simple, convenient and low-cost high throughput immunofluorecence assay technique is established and is used for evaluating the activities of cryptosporidium parvum and giardia in the ultraviolet (UV) disinfectant fluid; evaluation of the activities of cryptosporidium parvum and giardia in the UV irradiated water is realized by mainly carrying out competitive immunobinding on anti-pyrimidine-dimer (TDs) monoclonal antibodies and the TDs, taking fluorescein isothiocyanate as a marker and immunoglobulin (IgG) as a fluorescent substrate and binding 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI); compared with the fluorescent staining method for evaluating the effect of UV on inactivating cryptosporidium parvum and giardia, the method provided by the invention has such excellent characteristics as strong specificity, high sensitivity and low professional operation requirements; and the cryptosporidium parvum and giardia activity evaluation method system is perfected by binding the method provided by the invention with the fluorescent staining method and other methods, and the method provided by the invention can be widely applied to the fields such as water quality monitoring, disease control and epidemic prevention and the like.

A transcriptional activator of T. gondii is provided which comprises the tetracycline repressor (TetR) operatively linked to a transacting factor of T. gondii. Strains of T. gondii transformed with a vector containing such a transactivator may be used to prepare vaccine compositions or to identify essential genes in the parasite. The system provided may be useful in other Apicomplexan species such as Plasmodium falciparum, Plasmodium vivax, Plasmodium berghei, Plasmodium yoelii, Plasmodium knowlesi, Trypanosoma brucei, Entamoeba histolytica, and Giardia lambia.

The invention relates to a method for enriching and purifying cryptosporidium and giardia in water and a method for measuring the content of the cryptosporidium and giardia. The method comprises the following steps: adding calcium chloride and sodium bicarbonate into a water sample, regulating the pH of the water sample, and settling and enriching the cryptosporidium and giardia along with the generated calcium carbonate under alkaline conditions; collecting the precipitate, adding a sulfamic acid solution, performing acid dissociation, and performing density gradient centrifugal treatment to obtain a purification solution of the cryptosporidium and giardia; and finally, adsorbing the cryptosporidium and giardia to a cellulose acetate membrane through filtering, performing fluorescence microscope detection after dyeing treatment, and counting. According to the method, a method for concentrating, separating and purifying the cryptosporidium and giardia in the water sample is improved, the interference of background impurities during microscopic examination is reduced, the detection method is high in recovery rate and accurate in detection result, and a quality control standard which is formulated in USA Environmental Protection Agency and is used for measuring the cryptosporidium and giardia in the water is met.

A water treatment process whereby heat is used and recaptured to pasteurize water and or sewage effluent. Contaminated waters are partially treatable utilizing bacterial and chemical processes; however some bacteria (e.g. Cryptosporidium and Giardia) are resistant to chemicals. The present process raises the temperature of the water to a desired pasteurization temperature and for a corresponding required length of time to destroy pathogens and viruses. A heat exchanger is employed to use the waste heat from the pasteurization tank to pre-heat the fluid entering the tank. After the initial heating of the tank, the pre-heating reduces the required heat to maintain the temperature of the tank. Make-up heat increases the temperature within a small temperature range. The make-up heat may be waste heat from a separate process.

The invention discloses a method for enriching and purifying cryptosporidium and giardia in alga-containing water, and a method for determining the content of cryptosporidium and giardia. The enrichment and purification method comprises the following steps: pressurizing alga-containing water, precipitating to remove algae, filtering the obtained supernatant by using a mixed acetate fiber filter membrane, dissolving enriched products in acetone, centrifuging to obtain an enriched precipitate containing cryptosporidium and giardia, adding a phosphate-tween buffer solution to the precipitate to prepare a cryptosporidium and giardia suspension, carrying out density gradient centrifuge treatment to obtain a cryptosporidium and giardia purified solution, filtering to make cryptosporidium and giardia adhered to the acetate fiber membrane, dyeing, carrying out fluorescence microscope detection, and counting. The enrichment and purification method is an improved method for concentrating, separating and purifying cryptosporidium and giardia in an alga-containing water sample, and reduces the interference of background impurities during microscope detection, and the detection method has the advantages of high recovery rate and accurate detection result, and accords with Quality Control Standard of Cryptosporidium and Giardia in Water prescribed by Environmental Protection Agency in USA.

The present invention is directed to the recovery of bacteria and microparasites, particularly Cryptosporidium and Giardia, from water samples by filtration through a mixed cellulose ester membrane, partial dissolution of said membrane with methanol followed by completion in the presence of acetone, and purification and concentration using glass beads as a secondary confinement matrix.

The invention relates to a filtration and concentration method for improving the detection recovery rate of cryptosporidium and giardia in the technical field of water environmental water quality monitoring. The method comprises the following steps: sampling and filtering; adding elution; ultrasound oscillation and centrifugal treatment; carrying out stained preparation by sequentially adopting an immunomagnetic separation method after completing the centrifugal treatment; and then counting by a fluorescence microscope, and making statistics for the recovery rate. The method improves certain operation steps in the filtration and concentration stages, so as to achieve the purposes of reducing the detection cost of the cryptosporidium and giardia and improving the recovery rate.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

The present invention relates to the provision of polymers that are selected to have either; surface properties that allow protozoa, in particular Cryptosporidium and Giardia, to bind to the polymer; or have surface properties that are repellent to the binding of these protozoa. Methods for identifying suitable polymers are provided. Products comprising, consisting of or coated with the polymers of the present invention are also provided, as well as methods of treating or monitoring water employing polymers of the present invention.

The invention discloses a drug containing sustained-release ornidazole drug and used for treating infections of trichmonad, amoeba, giardia and anaerobic organism in gynecopathy as well as the preparation method thereof. The drug aims to provide a sustained-release ornidazole dropping pill formulation which has excellent curative effect, low toxic and side effects, broad-spectrum antibacterium, safer use, low frequency of drug taking and long effective hours and is prepared by adopting the solid dispersion technology, thereby thoroughly changing the defects existing in conventional oral formulations from the drug structure. The sustained-release ornidazole dropping pill adopts ornidazole as the ingredient and is prepared jointly with the medicinal carrier used as the stroma.

A water treatment process whereby heat is used and recaptured to pasteurize water and or sewage effluent.Contaminated waters are partially treatable utilizing bacterial and chemical processes, however some bacteria (e.g. Cryptosporidium and Giardia) are resistant to chemicals. Hence, the present process raises the temperature of the water to any desired temperature and for any desired length of time that would destroy most pathogens and viruses, including Cryptosporidium and Giardia, without the use of chemicals.