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Method of accelerating sample treatment with addition of graphene

A graphene and sample technology, applied in the field of sample processing technology, can solve the problems of insufficient freshness and unreliability of samples, and achieve the effect of shortening sample processing time and reducing sample processing time

Active Publication Date: 2015-10-14
BEIJING LEAGENE BIOTECH CO LTD
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Problems solved by technology

[0006] In order to overcome the limitation that the samples are not fresh enough and the results are unreliable due to the long sample processing period, the present invention "a method of adding graphene to accelerate sample processing" combines a new material graphene with sample processing Combined theoretical innovation technology to accelerate the processing of biological samples and pathological samples

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specific Embodiment 1

[0026] Specific embodiment one: graphene improved hematoxylin eosin (HE) staining solution

[0027] Hematoxylin and Eosin combined staining, referred to as HE staining, is the most basic staining method in routine pathological slide preparation and is widely used. Hematoxylin is a light yellow-brown crystal extracted from Sapphire native to Central and South America. It is an alkaline staining agent that generates hematoxylin after being oxidized. It is the same as mordant (commonly used is divalent aluminum or iron salt) ) together to stain the nuclei. In pathological diagnosis, teaching and scientific research, HE staining is often used to observe the morphology and structure of normal and diseased tissues. To determine or identify certain abnormal substances and special components in diseased tissues and cells, the special staining methods, enzyme histochemical methods, and immunohistochemical methods that need to be used are also based on the observation of HE-stained tis...

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Abstract

A method of accelerating sample treatment with addition of graphene belongs to the technical field of biological sample treatment and pathology. The invention particularly relates to the method of accelerating a sample treatment process with addition of graphene, by that the required time of the sample treatment process, such as dehydration, fixation, staining and the like. The method mainly solves a problem of a poor reliability of results since excessive long time of operation of sample treatment is required so that the sample in not fresh enough in the prior art. In the technical scheme, theoretical innovation of combining the new material, graphene, with the sample treatment is disclosed, namely, by means of the characters of adsorbing various atoms and molecules and being high in specific surface of the graphene, the sample treatment process is accelerated by carrying active components rapidly into the samples such as tissue, blood, cell and microorganisms and the like. The method is mainly used in treatment of various samples in clinical and scientific fields, comprising fixation, staining, blue returning, dehydration, transparence and sealing and the like on tissue samples (such as human, animal or plant samples) and fixation, staining and sealing and the like on samples such as blood, cells and microorganisms and the like.

Description

Technical field: [0001] The invention belongs to the technical fields of biological sample processing technology and pathology, and in particular relates to a method for accelerating the sample processing process after adding graphene, and a sample processing technology for shortening the time of dehydration, fixation, dyeing and the like. Background technique: [0002] Sample processing technology is developed and matured along with the progress of natural sciences (such as biology, medicine, etc.), especially with the application of clinical pathology technology. The ancient Greek medical master Hippocrates was the founder of Western medicine. His autopsy research obtained a lot of basic knowledge about human body structure and opened the way for the processing of pathological samples. In the middle of the 18th century, Italian Morgagni founded organ pathology based on accumulated autopsy experience, marking the beginning of pathomorphology. In the middle of the 19th cent...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 刘涛王红潘媛颖张伟谊郭喜李玉霞
Owner BEIJING LEAGENE BIOTECH CO LTD
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