Recombinant expression vector of excretion exudation protein of lung fluke, engineering bacterium, preparation method and uses thereof

Abstract

The invention discloses a recombinant expression vector for a lung fluke excretion and secretion protein, an engineering strain, a preparation method and an application thereof. The recombinant expression vector contains the encoding gene of the lung fluke excretion and secretion protein, the nucleotide sequence of the coding gene is shown as SEQ ID No.1, and the expression vector is pQE-80L. Theengineering strain contains the recombinant expression vector, and a host strain is bacillus coli. The method for preparing the lung fluke excretion and secretion protein through the recombinant expression vector comprises four steps of: constructing the engineering strain, culturing, inducting and expressing, and purifying an expression product. The lung fluke excretion and secretion protein canbe used for preparing agents or devices for detecting a paragonimiasis The recombinant expression vector and the engineering strain have the advantages of high expression amount and stable expression, and the lung fluke excretion and secretion protein prepared by the method has high protein yield and high immunoreactivity.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a recombinant expression vector, an engineering bacterium, a preparation method and an application of a paragonimiasis excretion and secretion protein. Background technique [0002] Paragonimiasis is a common and important zoonotic parasitic disease, scattered and prevalent in more than 10 provinces, municipalities, and autonomous regions in my country. The main pathogenic species are Paragonimus westermani and Pagumogonimus skrjabini, especially Pagumogonimus skrjabini, is a fluke that is only prevalent in my country. It cannot mature in the human body, but migrates and disturbs in the human body as larvae, often causing migratory subcutaneous Masses or nodules, brain space-occupying lesions and visceral damage and other manifestations of extrapulmonary parasites, causing damage to multiple organs and tissues. Due to atypical clinical symptoms, paragonimiasis is easily confused...

AI Technical Summary

Problems solved by technology

[0004] Due to the limited source of natural lung fluke ESP protein, it is difficult to produce on a large scale and cannot meet the needs of clinical immunodiagnosis, and the use of molecular biology methods and techniques to clone cDNA, construct recombinant expression vectors and induce expression of lung fluke ESP protein is the solution to this problem. Therefore, there is an urgent need in this area to develop recombinant expression vectors, engineering bacteria and preparation methods that can efficiently express the ESP protein of Lungworm

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