Preparation method of kit for early-to-mid rapid diagnosis of ovarian cancer

Abstract

The invention discloses a preparation method of a kit for early-to-mid rapid diagnosis of an ovarian cancer. The method comprises the following steps: 1. preparation of antigens: required antigens are prepared by adopting genes of galectin-3, transthyretin, carcinoma antigen-125 and human epididymis protein 4; 2, preparation of capture antibodies: the antigens are used for immunization of a mammal so as to obtain corresponding monoclonal antibodies; 3, preparation of detection antibodies: the antigens are used for immunization of the mammal so as to obtain corresponding polyclonal antibodies; 4, coating of the capture antibodies; 5, sealing and sample adding. The method has the benefits as follows: the ovarian cancer can be detected in the most comprehensive manner, the benign and malignant of a pelvic lump before menopause or after menopause can be better judged, differential diagnosis can be better performed on gynecology benign ovary lump and cyst as well as the ovarian cancer, the method has the characteristics of high sensitivity and high accuracy, and the sensitivity and accuracy can reach over 96 percent.

Description

Technical field: [0001] The invention relates to the field of kits for cancer diagnosis and detection, in particular to a method for preparing a kit for early and mid-stage rapid diagnosis of ovarian cancer. Background technique: [0002] Ovarian malignant tumor is one of the common malignant tumors of female reproductive organs, which refers to malignant tumors growing on the ovary, and 90% to 95% of them are primary ovarian cancers. 60% to 70% of them are advanced when they go to the doctor, and the curative effect of advanced cases is not good. Therefore, although the incidence rate of ovarian cancer is lower than cervical cancer and endometrial cancer, ranking third among gynecological malignancies, its mortality rate exceeds the sum of cervical cancer and endometrial cancer, ranking first among gynecological cancers, and is a serious threat to women. greatest disease of health. [0003] Due to the complex embryonic development, tissue anatomy and endocrine function of...

AI Technical Summary

Problems solved by technology

At present, the diagnostic kits for ovarian cancer in hospitals are used to detect common tumor markers, and their sensitivity and accuracy are low. Even joint inspections are only about 70%, which is difficult to meet the requirements of early and rapid diagnosis.

[0005] Since there is no rapid and efficient diagnostic kit for ovarian cancer on the market, it seriously affects the early detection and treatment of ovarian cancer. When ovarian cancer patients are diagnosed, they are often in the advanced stage of cancer, missing the best time for treatment, and greatly reducing the survival of patients. Rate

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