The invention discloses iodine-containing polysaccharide, which is characterized in that the molecular structure of the iodine-containing polysaccharide takes hydrophilic natural polysaccharide as a skeleton, and the structure general formula is shown in the description, wherein R is selected from hydrogen or derived triiodo benzoyl and is not all hydrogen; the structural formula of the derived triiodo benzoyl is shown in a description, wherein R minute is selected from one or two of hydrogen, methyl, methylamino, acetamido, N minute-methyl carbamoyl, methoxyl acetamido, N-methyl acetamido or alkyl. The high-substituted iodine-containing polysaccharide provided by the invention has the outstanding advantages, relative to iodine-containing micromolecule, that after the iodine-containing micromolecule is polymerized, cytotoxicity is greatly reduced, and the problem of micromolecule leakage caused by diffusion is avoided; in addition, a CT contrast agent provided by the invention is high in iodide content, good in contrast effect, low in cytotoxicity, and capable of being used as a blood vessel X-ray CT contrast agent for clinical CT examination and diagnosis.