Biological membrane-wrapped multi-mode tumor contrast agent as well as preparation method and application thereof

A biofilm and multi-modal technology, applied in the direction of general/multifunctional contrast agents, antineoplastic drugs, preparations for in vivo experiments, etc., can solve the problems of lack of early diagnosis methods and difficulties in early diagnosis of deep tumors, and achieve improved Tissue penetration and the ability to be taken up by target cells, enhance tumor targeting, and enhance the effect of contrast

Inactive Publication Date: 2018-09-18
JINAN UNIVERSITY
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, there is still a lack of ideal and specific ear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological membrane-wrapped multi-mode tumor contrast agent as well as preparation method and application thereof
  • Biological membrane-wrapped multi-mode tumor contrast agent as well as preparation method and application thereof
  • Biological membrane-wrapped multi-mode tumor contrast agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1 Preparation of melanoma targeted contrast agent

[0037] (1) Preparation of superparamagnetic ferritin nanocage (HFn)

[0038] The positive strains expressing superparamagnetic ferritin nanocages were constructed by Hubei Bios Biotech Co., Ltd., which provides services such as plasmid construction and the cells are also sourced from the company.

[0039] Extract human skeletal muscle cell RNA, reverse transcribed into cDNA as a template; amplify human FTH1 gene, PCR primers are as follows (black underline is restriction site)

[0040] F:5’A GTC GCC CAT ATG ACG ACC GCG TCC 3’(Nde I)

[0041] R: 5’GCC GGA TCC TTA GCT TTC ATT ATCA C 3’(Bam HI)

[0042] After the PCR product was purified and recovered, it was ligated with pET-30a(+) vector by double enzyme digestion, and the product was transformed into XL2-Blue competent cells. The product was screened by Kan+ resistance plate, and the colony was identified and sent to sequencing. The plasmids with correct sequencing ...

Example Embodiment

[0053] Example 2 Preparation example of glioma targeted contrast agent

[0054] (1) Preparation of superparamagnetic ferritin nanocage of ferroferric oxide:

[0055] Preparation of ferritin nanocages of superparamagnetic ferroferric oxide: add degassed 8.0mL, 100mM NaCl solution to the reaction flask (argon protection, strictly anaerobic operation), and then add 2.0mg, 3.9nmol of superparamagnetic iron Protein nanocage (HFn1mg / mL) was added, the temperature was controlled at 65℃, pH=8.5, 12.5mM, 1566 μL degassed ferrous ammonium sulfate, with a stoichiometric ratio of H 2 O 2 :Fe 2+ =1:3 to prepare 4.17mM, 1566μL degassed hydrogen peroxide solution, 25mM, 1566μL NaOH solution to neutralize the H generated during the reaction + To maintain pH=8.5; the three were added at a constant rate of 31.3μL / min at the same time, theoretically loading 5000Fe; after all were added, the reaction lasted 5min; after the reaction was completed, 200μL (300mM) sodium citrate was added to remove the fre...

Example Embodiment

[0062] Example 3 Stability Test of Nanoparticles

[0063] Using the theoretically loaded 1000Fe, 3000Fe, 5000Fe superparamagnetic ferritin nanocages prepared in Example 1, the whole blood of human blood type O was processed to obtain a biofilm 10mg dissolved in 1mL water and 100μL physiological saline (including red blood cells). Membrane, white blood cell membrane and platelet membrane). When red blood cells are used for hypotonic swelling and hemolysis, many membrane pores with a diameter of 20-50 nm will be formed on the cell membrane. M-HFn is added, incubated for 30 minutes, centrifuged, and the precipitate is collected. Load a red blood cell ghost suspension of M-HFn. Next, the red blood cell ghosts were successively extruded with 400nm, 200nm, 100nm or 50nm liposome preparation equipment (Avanti mini extruder) to prepare RBC-1000Fe, RBC-3000Fe and RBC-5000Fe aqueous solutions, respectively.

[0064] Take 1 mL each of RBC-1000Fe, RBC-3000Fe and RBC-5000Fe aqueous solutions, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sizeaaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a biological membrane-wrapped multi-mode tumor contrast agent as well as a preparation method and application thereof. The biological membrane-wrapped multi-mode tumor contrastagent comprises a biological membrane, a ferritin nanometer cage (M-HFn) of superparamagnetic ferroferric oxide and a fluorescent dye, wherein the ferritin nanometer cage (M-HFn) of the superparamagnetic ferroferric oxide and the fluorescent dye are wrapped with the biological membrane and are connected with each other to form a fluorescent dye-M-HFn compound, a bionic nanometer biological membrane vector delivery system in vivo is constructed, in vivo long circulation can be realized, targeting to a tumor is strengthened, non-specific accumulation is reduced, damages on normal tissues are reduced, and the contrast effect is strengthened at the same time. The invention also provides a preparation method of the biological membrane-wrapped multi-mode tumor contrast agent, and the preparationmethod has important application prospect and study value in the field of clinic oncology early diagnosis and treatment and the like.

Description

technical field [0001] The invention belongs to the field of nuclear magnetic resonance contrast agents, in particular to a biofilm-wrapped multimodal tumor contrast agent and its preparation method and application. Background technique [0002] Cancer is one of the biggest killers threatening human life around the world, and it is a major challenge in the field of medical research. Magnetic resonance imaging has become an important basis for the diagnosis of primary tumors and metastases. The specificity and sensitivity of current tumor detection methods are low, and it is impossible to achieve specific recognition of tumor cells and controllable delivery of drugs at the same time. With the continuous development of nanotechnology, nanofluorescent probes have been widely used in chemistry, biology, medicine and other fields, especially in the diagnosis, imaging and treatment of tumors. The detection and imaging of multiple tumor markers with high sensitivity and high sele...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K49/18A61K49/00A61K9/50A61K47/46A61P35/00
CPCA61K9/5068A61K49/0002A61K49/0021A61K49/0097A61K49/1866A61K49/1896A61P35/00
Inventor 戴箭宋镕光阮淼亮薛巍
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap