1 results about "Longitudinal Relaxation Rate" patented technology

This invention discloses a pre-targeting probe assembly for in vivo unstable iron pool imaging, its preparation method, and its application. The pre-targeting probe assembly includes Fe... 2+ Response paramagnetic probe GdEpTz and fluorescent reporter probe 2E-Tco-Hcy, Fe 2+ Response paramagnetic probe GdEpTz with Fe 2+ Following interaction with endogenous proteins, free radicals are generated and covalent cross-linking occurs, significantly increasing the longitudinal relaxation rate (r1) by approximately 104.1%. Simultaneously, tetrazine-based Tz is efficiently enriched in the lesion region. The enriched Tz captures the fluorescent signal reporter probe 2E-Tco-Hcy via a highly efficient IEDDA bioorthogonal reaction, triggering a 155.5-fold significant enhancement of the fluorescence signal. This invention introduces a bioorthogonal pre-targeting strategy based on the reverse electron demand Diels-Alder (IEDDA) reaction, aiming to achieve Fe in deep tissues through the organic integration of "target-induced activation" and "bioorthogonal chemistry." 2+ Dynamically changing, highly selective, and high-contrast synchronous visual monitoring.