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A contrast agent for fluorescence-magnetic resonance dual-mode targeted biological imaging and its preparation method

A biological imaging and magnetic resonance technology, applied in the field of nanomaterials, achieves the effect of loose reaction conditions and simple methods

Active Publication Date: 2019-04-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] To sum up, the problem with the above-mentioned technology is that if a better contrast effect is to be achieved, the content of gadolinium in the carbon quantum dots is relatively high, and gadolinium is highly toxic. Therefore, the technical problem to be solved in this application is: how to Improve MRI contrast effect and maintain excellent fluorescence imaging ability while maintaining low gadolinium content

Method used

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  • A contrast agent for fluorescence-magnetic resonance dual-mode targeted biological imaging and its preparation method
  • A contrast agent for fluorescence-magnetic resonance dual-mode targeted biological imaging and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of Gd-doped carbon dots (GCD):

[0050] A. Preparation of DTPA-Gd: Weigh 0.45gDTPA (diethyltriaminepentaacetic acid, chelating agent) and 0.3gGdCl respectively 3 Dissolve in 10mL pure water, stir and react at 40°C for 3h, and recover for use after the reaction is completed;

[0051] B. Weigh 1.0g of citric acid monohydrate (CA) and 0.5g of polyethyleneimine (PEI, molecular weight: 1800) in a 100mL beaker, add 5mL of pure water to dissolve, then add 5mL of the above-mentioned DTPA-Gd solution and stir evenly;

[0052] C. Place the beaker containing the above mixed solution on a heating plate, heat at 180°C until the solution is nearly evaporated to dryness, add 1mL of pure water to the beaker, continue heating until the solvent is nearly evaporated to dryness, repeat adding 1mL of pure water and evaporate to nearly dryness The operation steps of 10 times, obtain brown-yellow colloidal substance;

[0053] D. Dissolve the obtained brown-yellow substance in 10...

Embodiment 2

[0060] Preparation of Gd-doped carbon dots (GCD):

[0061] A. Preparation of DTPA-Gd: Weigh 0.26gDTPA (diethyltriaminepentaacetic acid, chelating agent) and 0.2gGdCl respectively 3 Dissolve in 10mL pure water, stir and react at 40°C for 3h, and recover for use after the reaction is completed;

[0062] B. Weigh 1.0g of citric acid monohydrate (CA) and 0.4g of polyethyleneimine (PEI, molecular weight: 1800) in a 100mL beaker, add 5mL of pure water to dissolve, then add 5mL of the above-mentioned DTPA-Gd solution and stir evenly;

[0063] C. Place the beaker containing the above mixed solution on a heating plate, heat at 180°C until the solution is nearly evaporated to dryness, add 1mL of pure water to the beaker, continue heating until the solvent is nearly evaporated to dryness, repeat adding 1mL of pure water and evaporate to nearly dryness The operation steps of 10 times, obtain brown-yellow colloidal substance;

[0064] D. Dissolve the obtained brown-yellow substance in 10...

Embodiment 3

[0071] Preparation of Gd-doped carbon dots (GCD):

[0072] A. Preparation of DTPA-Gd: Weigh 1.3gDTPA (diethyltriaminepentaacetic acid, chelating agent) and 0.5gGdCl respectively 3 Dissolve in 10mL pure water, stir and react at 40°C for 3h, and recover for use after the reaction is completed;

[0073] B. Weigh 1.0g of citric acid monohydrate (CA) and 0.8g of polyethyleneimine (PEI, molecular weight: 1800) in a 100mL beaker, add 5mL of pure water to dissolve, then add 5mL of the above-mentioned DTPA-Gd solution and stir evenly;

[0074] C. Place the beaker containing the above mixed solution on a heating plate, heat at 180°C until the solution is nearly evaporated to dryness, add 1mL of pure water to the beaker, continue heating until the solvent is nearly evaporated to dryness, repeat adding 1mL of pure water and evaporate to nearly dryness The operation steps of 10 times, obtain brown-yellow colloidal substance;

[0075] D. Dissolve the obtained brown-yellow substance in 10 ...

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Abstract

The invention discloses a contrast medium for fluorescent-magnetic resonance dual-mode targeting biological imaging and a preparation method thereof. The method comprises the following steps that 1, a chelating agent and a compound containing Gd3+ react in water to obtain a Gd3+ chelate solution; 2, citric acid and polyethyleneimine are dissolved into water, and then the Gd3+ chelate solution is added to be mixed; 3, the mixture obtained in the step 2 is dissolved and evaporated to dryness repeatedly, centrifuging is performed on the dissolved and dried products, a supernate is taken for dialysis in water to obtain a dialysis solution, and a dialysis solution is subjected to freeze-drying, and Gd doped carbon dots are obtained. Meanwhile, the invention further discloses a contrast medium for fluorescent-magnetic resonance dual-mode targeting biological imaging. The contrast medium is synthetized in an environment-friendly mode, is low in gadolinium content, promotes surface functionalization, and has the quite high longitudinal relaxation rate and the fluorescence quantum yield, and the good biological compatibility and blood compatibility.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a contrast agent for fluorescence-magnetic resonance dual-mode targeted biological imaging and a preparation method thereof. Background technique [0002] Non-invasive molecular imaging technology can visualize cell function and molecular reaction process without damaging living tissue, so it has attracted extensive attention in the fields of early diagnosis of cancer and basic research. At present, there are many non-invasive molecular imaging methods clinically. Although each imaging method has the ability of independent imaging, each has its own shortcomings, which makes it difficult to rely on a single imaging mode for accurate and rapid data acquisition. [0003] At the same time, Chinese patent application CN201410012621.1 discloses a gadolinium (Ш)-carbon quantum dot and its preparation method and its application in magnetic resonance-fluorescence dual-mode imaging p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/06
CPCA61K49/0052A61K49/06
Inventor 易长青潘益方雅宁
Owner SUN YAT SEN UNIV
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