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32 results about "Spin labeled" patented technology
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Site-specific labeling of proteins for NMR studies
Methods of producing and / or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase / tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided.
Owner:THE SCRIPPS RES INST +1
Hybrid phosphoinositide phospholipids: compositions and uses
InactiveUS20050148042A1Facilitate recruitment of PtdlnsPPeptide/protein ingredientsMicrobiological testing/measurementLipid formationChemical Moiety
The methods and compositions disclosed herein concern the synthesis of a novel class of “two-headed” phospholipid-phosphoinositide hybrids possessing a carbon backbone, such as 2,3-diacylthreitol, erythritol or a synthetic module. The second phospholipid head group allows introduction of a biochemical or chemical moiety in a position orthogonal in space to those occupied by the phosphoinositide head group and the two acyl chains. The diacyl moieties allow for the incorporation of Pea-PIP2 into a lipid bilayer, while the Ptdlns(4,5)P2 moiety in the aqueous layer is specifically recognized by lipid binding proteins. In alternative embodiments of the invention, reporters, for example biotin, fluorophores and / or spin labels, are attached to the free amino group of the head groups of such molecules to specifically target the reporters to the lipid-water interface.
Owner:PRESTWICH GLENN +5
Arterial spin labeled, segmented, interleaved 3D grase MRI
ActiveUS20090212773A1High signal sampling rateImprove spatial resolutionMeasurements using NMR imaging systemsElectric/magnetic detectionMri imageSpin labeled
A magnetic resonance imaging (MRI) method of obtaining MRI images of a patient and storing the images in physical storage media.
Owner:ADVANCED MRI TECH
Arterial spin labeled, segmented, interleaved 3D GRASE MRI
ActiveUS7898254B2High signal sampling rateImprove spatial resolutionMagnetic measurementsElectric/magnetic detectionSpin labeled3d grase
A magnetic resonance imaging (MRI) method of obtaining MRI images of a patient and storing the images in physical storage media.
Owner:ADVANCED MRI TECH
Site-Specific Labeling of Proteins for Nmr Studies
Methods of producing and / or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase / tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided.
Owner:THE SCRIPPS RES INST +1
Site-specific labeling of proteins for NMR studies
Methods of producing and / or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase / tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided.
Owner:THE SCRIPPS RES INST & IRM
Arterial spin-labeled (ASL) multiplexed echo planar imaging (m-epi)
InactiveUS20130085379A1Reduce trainingIncreased complexityMagnetic measurementsSensorsSpin labeledPerfusion
An MRI system and method for imaging perfusion in an arterial spin labeled (ASL) process in which multiplexed echo-planar imaging (M-EPI) is used rather than conventional EPI, to thereby speed up imaging and generate sets of images that show different phases of perfusion and provide additional benefits. A single multiband RF excitation pulse can be used to excite multiple slices for imaging, or a time sequence of multiband pulses can be used to further increase the number of slices.
Owner:FEINBERG DAVID
Development of fluorescently p-loop labelled kinases for screening of inhibitors
The present invention relates to a kinase labelled at an amino acid naturally present or introduced in the P-loop of said kinase, wherein said labelling is effected at a free thiol or amino group of said amino acid and said label is (a) a thiol- or amino-reactive fluorophore sensitive to polarity changes in its environment; or (b) a thiol-reactive spin label, an isotope or an isotope-enriched thiol- or amino-reactive label, such that said fluorophore, spin label, isotope or isotope-enriched label does not inhibit the catalytic activity and does not interfere with the stability of the kinase. The invention furthermore relates to a method of screening for kinase inhibitors, a method of determining the kinetics of ligand binding and / or of dissociation of a kinase inhibitor and a method of generating mutated kinases suitable for the screening of kinase inhibitors using the kinase of the present invention.
Owner:MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN EV
System and Method for Hybrid Radiofrequency Labeling for Magnetic Resonance Imaging
ActiveUS20120296193A1Improve signal-to-noise ratioMagnetic measurementsDiagnostic recording/measuringMedical imaging dataSpins
A system and method is provided for magnetic resonance angiography (MRA) that includes applying a first labeling pulse sequence to a first labeling region having a first portion of a vasculature of a subject extending through the first labeling region to label spins moving within the first labeling region. A second labeling pulse sequence is applied to a second labeling region having a second portion of a vasculature of the subject extending through the second labeling region to label spins moving within the second labeling region. The first and second labeling pulse sequences include different labeling techniques. An imaging pulse sequence is applied to an imaging region having a third portion of a vasculature of the subject extending through the imaging region that is displaced from the first and second labeling region to acquire imaging data from the spins labeled by the first labeling pulse sequence and the second labeling pulse sequence. An MRA image is reconstructed of at least the third portion of the vasculature of the subject from the medical imaging data.
Owner:NORTHSHORE UNIV HEALTHSYST
Mutants of streptokinase and their covalently modified forms
ActiveUS20100034804A1Proteolytic stability is enhancedExtended plasma elimination half-lifeBacteriaPeptide/protein ingredientsFluorescenceHalf-life
The present invention relates to novel mutants of Streptokinase, its functional fragments and covalently modified forms. Methods are provided for the preparation of the bacterial plasminogen activator protein, Streptokinase its muteins, species variants and their covalently modified variants that are characterized by improved therapeutic properties, such as increased proteolytic stability, extended plasma half-lives, reduced immuno-reactivity and enhanced fibrin clot specificity. The method involves either incorporating additional cysteine residues, or substituting cysteine residues for naturally occurring amino acids into non-essential regions of the protein such that the catalytic activity of the resultant protein remains largely unaltered. These cysteine variants were further modified by covalently attaching a cysteine reactive polymer such as polyethylene glycol (PEG) or sulfhydryl-reactive moieties from a group that includes fluorophore, spin labels or other small conjugates. Disclosed herein are site-specific biologically active conjugates of Streptokinases and its covalently modified variants.
Owner:COUNCIL OF SCI & IND RES
Magnetic resonance artery spin labeling cerebral perfusion imaging data artifact graph removal method
ActiveCN106127792AReduce mistakesImprove detection accuracyImage enhancementImage analysisImaging qualitySpin labeled
The invention discloses a magnetic resonance artery spin labeling cerebral perfusion imaging data artifact graph removal method. The method comprises steps that a structure image of a detected brain is acquired, the structure image is segmented to generate a grey matter probability graph GM and a white matter probability graph WM, the grey matter probability graph GM and the white matter probability graph WM form a reference image pmCBF, and artifact graphs of rheoencephalograms CBF1-CBFn at n time points of the detected brain are removed by utilizing the reference image pmCBF. The method is advantaged in that the artifact graphs of the rheoencephalogram sequence can be effectively removed, error reduction is realized, and image quality is improved.
Owner:HANGZHOU NORMAL UNIVERSITY
Endohedral fullerenes as spin labels and MRI contrast agents
InactiveUS20070048870A1Good techniqueHigh sensitivityNanomedicineFibre chemical featuresMRI contrast agentResonance
Owner:INTEMATIX
Nitrone, nitroso, and nitroxide spintraps and spin labels and their hydroxylamines
Nitrone, nitroso, and nitroxide spintraps and spin labels and their reduction products are claimed for the prevention and treatment of fibrocystic disease of breast, premenstrual dysphoric syndrome and associated symptomology, prevention and treatment of migraine headache, cyclic vomiting syndrome, rectal hemorrhoids, trigeminal neuralgia, peripheral vascular disease, influenza, peridontitis and gingivitis, herpes zoster, herpes simplex, and post-herpetic neuralgia.
Owner:PROCTOR PETER H
Compositions and methods to assess the capacity of hdl to support reverse cholesterol transport
InactiveUS20140162376A1Nervous disorderMeasurements using double resonanceReverse cholesterol transportSpectroscopy
Owner:CHILDREN S HOSPITAL &RES CENT AT OAKLAN
Oligonucleotide labeling reactants based on acyclonucleosides and conjugates derived thereof
The invention relates to a labeling reactant of formula (I) useful for labeling an oligonucleotide wherein: R is a temporary protecting group. A is either a phosphorylating moiety or a solid support tethered to Z via a linker arm E''. Z is a bridge point and is formed from E is a linker arm between R and Z. E' is a linker arm between Z and Z'. E'' is a linker arm between Z and A. E''' is a linker arm between Z' and G. Z' is a purine or pyrimidine base. G is a protected bivalent aromatic structure, tethered to two iminodiacetic acid ester groups N(CH2COOR'')2, or G is a structure selected from the group consisting of or G is a protected functional group, or G is a protected or unprotected organic dye, hapten or a spin label.
Owner:WALLAC
Method for Treatment of Radiologically-Dense Breasts
ActiveUS20170000776A1Nitro compound active ingredientsHeterocyclic compound active ingredientsMedicineSpins
Owner:PROCTOR PETER HERBERT +1
Spin labeled fluorescent probe with single electron structure and application thereof
InactiveCN106753334AMild reaction conditionsImprove permeabilityOrganic chemistryFluorescence/phosphorescenceFluorescenceOxidation resistant
The invention discloses a spin labeled fluorescent probe with single electron structure. The spin labeled fluorescent probe with single electron structure is prepared by the following method: (1) adding rhodamine B and 4-hydroxy-2, 2, 6, 6,-tetramethyl-piperidine nitrogen oxygen free radical into a reaction solvent dichloromethane, adding 4-dimethylaminopyridine to carry out reaction for 20min, then adding N, N'-dicyclohexyl carbodiimide, carrying out reaction at room temperature under argon protection for 24h; and (2) at the end of the reaction, removing the solvent, and subjecting the remaining materials to column chromatography separation: adopting dichloromethane / methanol=20 / 1 (v / v) as the eluant, taking 200-mesh neutral alumina as the stationary phase, collecting eluent, and removing the eluate. The spin labeled fluorescent probe with single electron structure provided by the invention has good cell permeability and low cytotoxicity, can be used for detection of intracellular hydroxyl radicals, mercapto free radicals and, cellular redox environment and oxidation resistance evaluation research of polyphenolic antioxidants.
Owner:CHINA PETROLEUM & CHEM CORP +1
Electron Spin Labeled Ice Binding Compounds Used For Carrying Paramagnetic Centers For Dynamic Nuclear Polarization
ActiveUS20180044382A1Inhibit growthInhibition of recrystallizationDepsipeptidesIn-vivo testing preparationsSolubilityConfocal
Spin-labeled ice binding compounds (IBCs) including ice binding proteins (IBPs) or antifreeze proteins (AFPs) and their analogs may carry paramagnetic centers for dynamic nuclear polarization (DNP), for enhancing nuclear magnetic resonance (NMR) signal intensities. Use of spin-labeled IBCs to perform DNP exploits the IBCs' ability to homogeneously distribute the paramagnetic centers in frozen water solution at low temperature, leading to high DNP efficiency. Other advantages of using spin-labeled IBCs include cryo-protecting biological samples; cryo-preserving relative positions and orientations of the spin labeling groups; selecting positions and orientations of spin labeling groups with freedom and without technical barriers to making multiple spin labels in an IBC; and enabling use of a solvent that is primarily water for DNP at low temperatures in view of the potentially high water solubilities of spin-labeled IBCs.
Owner:TRUSTEES OF THE CALIFORNIA STATE UNIV
Nitroxide containing amyloid binding agents for imaging and therapeutic uses
Owner:RGT UNIV OF CALIFORNIA
Nitroxide containing amyloid binding agents for imaging and therapeutic uses
Owner:RGT UNIV OF CALIFORNIA
Method for site-specific protein modifications
ActiveUS20070020690A1Avoid modificationHydrolasesPeptide/protein ingredientsProtein moleculesFluorophore
Owner:RGT UNIV OF CALIFORNIA
Electron spin labeled ice binding compounds used for carrying paramagnetic centers for dynamic nuclear polarization
ActiveUS9738686B2Inhibit growthInhibition of recrystallizationDepsipeptidesIn-vivo testing preparationsSolubilitySolvent
Spin labeled ice binding compounds (IBCs) including ice binding proteins (IBPs), also called antifreeze proteins (AFPs) and their analogs are exploited to carry the paramagnetic centers for dynamic nuclear polarization (DNP), for enhancing nuclear magnetic resonance (NMR) signal intensities. Use of spin labeled IBCs to perform DNP exploits the IBCs' ability to homogeneously distribute the paramagnetic centers in frozen water solution at low temperature, leading to high DNP efficiency. Other advantages of using spin labeled IBCs include: (1) ability to cryo-protect biological samples; (2) the relative positions and orientations of the spin labeling groups in an IBC may also be cryo-preserved; (3) positions and orientations of spin labeling groups to an IBC can be selected with great freedom and without technical barrier to making multiple spin labels in an IBC; and (4) water solubilities of spin labeled IBCs are potentially high, enabling use of a solvent that is primarily water for DNP at low temperatures.
Owner:TRUSTEES OF THE CALIFORNIA STATE UNIV
Spinning label vision automatic splitting machine and control method thereof
ActiveCN107558158AReduce generationReduce hearing damageSevering textilesControl systemMotor control
The invention provides a spinning label vision automatic splitting machine and a control method thereof. When the automatic splitting machine performs slitting, the position of each cut line is photographed in real time through a camera, a computer can automatically compare current photographed position coordinates and last photographed position coordinates, calculate the displacement of the cut lines, displacement change information is transmitted to a motor control system in real time, the motor control system controls one or more motors to rotate to drive a synchronous belt to rotate, a sliding plate and splitting tools on the sliding plate are driven to move to the corresponding splitting positions along a guide rail, and therefore all the tools are located within the cutting range ofthe splitting lines during splitting, computer control is adopted, the splitting precision is improved, the product quality is improved, spinning label splitting inferior-quality products are greatlyreduced, the motor is adopted for automatically controlling the splitting position, no manual operation is needed, the working efficiency is improved, and the hearing loss of a worker is reduced.
Owner:广州创造智能设备科技有限公司
A kind of spin-labeled cameline A compound, preparation method and use thereof
The invention discloses a spin-labeling luotonin A compound of formula (I) as shown in the specification, as well as a preparation method and application of the compound. In-vitro antitumor activity screening results show that compared with luotonin A, the spin-labeling luotonin A compound disclosed by the invention is relatively intense in inhibition activity on human lung adenocarcinoma cells (A549), human breast cancer cell lines (MDA-MB-468), human ovarian neoplasm cells (SKOV3) and human colon cancer cell lines (HCT 116), and has good application prospects.
Owner:甘肃康扬医药科技有限公司
Multi-module arterial spin labeling magnetic resonance angiography method, device and equipment
The invention relates to the technical field of medical imaging, in particular to a multi-module arterial spin labeling magnetic resonance angiography method, device and equipment, and the method comprises the steps: entering a contrast stage in a magnetic resonance angiography period, applying a contrast multi-module pre-pulse to a target, and after a first delay time, executing a contrast stage; applying a three-dimensional damaged gradient echo sequence to a target, and collecting a magnetic resonance signal in a contrast stage; entering a marking stage after the contrast stage is ended, then applying a marking multi-module pre-pulse to the target, and after a second delay time, acquiring a magnetic resonance signal of the marking stage in the contrast stage; and after the marking stage is finished, entering a next magnetic resonance angiography period, and reconstructing a blood vessel image of the target according to the magnetic resonance signals of the contrast stage and the marking stage acquired in a plurality of periods. Therefore, the problems of insufficient intracranial blood marking, low marking efficiency, long marked blood transfer time and the like in related technologies are solved.
Owner:FUDAN UNIVERSITY
Longitudinal relaxation rate measuring system and measuring method using same
ActiveCN114778589AEnhanced signalEffective interactionAnalysis using nuclear magnetic resonanceBiological testingChemical reactionSpin labeled
The invention provides a longitudinal relaxation rate measurement system and a measurement method using the system. The system comprises a pump light unit, a confocal microscopic collection unit, a displacement unit, a microwave radiation unit, a static magnetic field unit, a diamond probe containing a nitrogen-vacancy color center and a computer control processing unit. The measurement method using the system comprises the following steps: S1, connecting a first biomolecule to hydrogel in a biochemical reaction manner; s2, cleaning the hydrogel after the operation in the step S1, dropwise adding second and third biomolecules with different spin labels onto the hydrogel for reaction, and then fully cleaning to obtain a to-be-detected sample of interaction of the first biomolecule, the second biomolecule and the third biomolecule; and S3, carrying out longitudinal relaxation rate measurement on the to-be-measured sample. The system can be used for measuring the interaction of biomolecules, and has the advantages of stable signal, high signal-to-noise ratio, capability of simultaneously detecting multi-channel biological samples and the like.
Owner:UNIV OF SCI & TECH OF CHINA
A method for removing artifact from magnetic resonance arterial spin-labeled cerebral perfusion imaging data
ActiveCN106127792BReduce mistakesImprove detection accuracyImage enhancementImage analysisImaging qualitySpin labeled
The invention discloses a magnetic resonance artery spin labeling cerebral perfusion imaging data artifact graph removal method. The method comprises steps that a structure image of a detected brain is acquired, the structure image is segmented to generate a grey matter probability graph GM and a white matter probability graph WM, the grey matter probability graph GM and the white matter probability graph WM form a reference image pmCBF, and artifact graphs of rheoencephalograms CBF1-CBFn at n time points of the detected brain are removed by utilizing the reference image pmCBF. The method is advantaged in that the artifact graphs of the rheoencephalogram sequence can be effectively removed, error reduction is realized, and image quality is improved.
Owner:HANGZHOU NORMAL UNIVERSITY
System and method for hybrid radiofrequency labeling for magnetic resonance imaging
Owner:NORTHSHORE UNIV HEALTHSYST
Method for site-specific protein modifications
A method for site-specific modification of protein molecules includes providing a protein molecule having at least first and second non-terminal Cys residues at respective first and second sites within the protein molecule, providing a blocking molecule that is suitable to interact with the protein molecule to selectively block the second non-terminal Cys residue to prevent modifications of the second non-terminal Cys residue while leaving the first non-terminal Cys residue unblocked, and providing a Cys-modifying molecule that is suitable to modify the first non-terminal Cys residue. The blocking molecule according to an embodiment of the invention may be a protein molecule, DNA, an aptamer or synthetic organic molecules. The Cys-modifying molecule according to an embodiment of the invention may be a fluorophore, biotin, spin label, a sugar or a non-natural amino acid analogue.
Owner:RGT UNIV OF CALIFORNIA
Method for treatment of radiologically-dense breasts
ActiveUS10028943B2Nitro compound active ingredientsHeterocyclic compound active ingredientsMedicineSpins
Owner:PROCTOR PETER HERBERT +1