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The application provides a sealant and a preparation method thereof, and relates to the technical field of sealants. The sealant comprises A component and B component. The A component comprises the following raw materials: bio-based polyaspartic ester resin, modified nano zinc oxide, tea polyphenol microcapsule, filler, dispersant and defoaming agent. The B component comprises the following raw materials: bio-based isocyanate prepolymer, HDI trimer, catalyst and light stabilizer. The sealant provided by the application has the comprehensive performances of weather resistance, anti-yellowing, mechanical balance, environmental protection, safety, antibiosis and durability, and is suitable for the sealing of building gaps in the environment exposed to light and moisture for a long time.