165results about How to "Good low toxicity" patented technology

The invention discloses a fluorescent glucose nano biosensor. Active ingredients of the sensor are functionalized fluorescent nanoparticles. The fluorescent glucose nano biosensor is prepared by combining an enzyme catalysis method and a fluorescent oxygen nano sensor. Fluorescence detection of glucose is indirectly realized based on consumption of glucose oxidase on oxygen in a glucose catalysis process. The glucose nano biosensor has the advantages of high response speed, high sensitivity and high stability, and the lower limit of glucose detection can be 0.5mmol/L. Because the sensor has a nanoscale size and high biocompatibility, the sensor can be used for detecting the concentration of glucose in microenvironments (such as living cells) in organisms and in human body fluid (such as plasma).

The invention relates to a marker-free gene-deletion attenuated mutant strain derived from an Edwardsiella tarda wild strain, constituting an attenuated live vaccine of the Edwardsiella tarda strain, wherein, chorismate synthase genes aroC of the Edwardsiella tarda strain are deleted; the Edwardsiella tarda strain is particularly an Edwardsiella tarda strain EIB202; and endogenous plasmids of the Edwardsiella tarda strain are further deleted. The invention further provides a preparation for the marker-free gene-deletion attenuated mutant strain derived from the Edwardsiella tarda wild strain, and the related application in preventing mariculture Edwardsiellosis. The marker-free gene-deletion attenuated mutant strain derived from the Edwardsiella tarda wild strain or the related preparation eliminates the potential security risk to the environment and products in most conventional attenuated live vaccines and constitutes a safe, effective and economic vaccine orienting to the mariculture Edwardsiellosis.

The invention discloses a detection kit for LDL (low-density lipoprotein) cholesterol and a use method of the detection kit, relates to the field of biochemical detection and aims to provide a detection kit, having high stability, accuracy and precision as well as low toxicity, for LDL cholesterol and a use method of the detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a surfactant, polyethylene glycol, SDS (sodium dodecyl sulfate), Emulgen A9 series, CHOD (cholesterol oxidase), CHER (cholesterol esterase), ascorbic acid oxidase, CAT (catalase), 4-AAP (4-aminoantipyrine) and the like; the reagent 2 is prepared from octylphenyl polyethylene glycol, cholate, a compound stabilizer, bovine serum albumin, POD (peroxidase), TOPS (sodium 3-(N-ethyl-3-methylanilino)propanesulfonate) and the like. The proclin series added to the kit is a novel biological preservative, has good compatibility with various enzymes, better stability and low toxicity, and the stability of the reagents is maintained.

The invention relates to a preparation method and application for natural latex packing. The natural latex packing is prepared by taking chitin and modified starch as main raw materials and by combining the technologies of freezing-thawing, ultrasound and the like with a chemical modifying method, and has double functions of reinforcing and resisting bacteria. The preparation method for the natural latex packing provided by the invention is simple in process, wide in raw material source and low in cost; the prepared natural latex packing is safe and environment-friendly, and can be used for partially replacing carbon black, white carbon black, raw rubber and the like in rubber industry; the pollution to the environment is reduced, and the production cost of a rubber product is reduced; moreover, rubber composite packing is endowed with a certain antibacterial property, so that the antibacterial effect of the rubber product is improved, the rubber quality is improved, and the application range of a rubber impregnated product is enlarged.

The invention relates to a carboxylation beta-cyclodextrin modified low-toxicity functional quantum dot and a preparation method thereof, belonging to the field of specific molecular recognition diagnostic reagent. In the invention, the preparation method of the carboxylation beta-cyclodextrin modified low-toxicity functional quantum dot comprises the steps that: a functional group carboxyl is connected on beta-cyclodextrin through a chemical modification method to obtain carboxy-beta-cyclodextrin; under the action of 1-ethyl-3-(3-dimethyl propylamine) carbodiimide hydrochloride and N-hydroxysuccinimide, activated folic acid is firstly coupled with amino-terminated poly (ethylene glycol) and then coupled with the carboxy-beta-cyclodextrin to obtain folic acid-beta-cyclodextrin; silver, zinc and other low-toxicity elements are utilized as raw materials to prepare an oil soluble infrared quantum dot, and the folic acid-beta-cyclodextrin is utilized to perform water-soluble modification on the oil soluble infrared quantum dot to obtain the carboxylation beta-cyclodextrin modified low-toxicity functional quantum dot. The quantum dot prepared by the method disclosed by the invention has good water solubility and low toxicity, and the emission spectrum is in a near infrared area, and the quantum dot can be used for specific fluorescence detection on tumors because of being coupled with the folic acid.

The invention discloses a linear-hyperbranched phosphate salt surface active agent and a preparation method thereof. AB2 type monomers are synthesized, then avterminal hydroxyl hyperbranched polymer is synthesized, a long chain is introduced into the middle end of the terminal hydroxyl hyperbranched polymer, unmodified hydroxyl continues to be phosphorylated, and the linear-hyperbranched phosphate salt polymer is synthesized. The hydroxyl long chain is introduced, so that lipophilicity is improved, terminal lipophilicity is modified into a phosphate base, the prepared polymer has multi-terminal hydrophilic groups and hydrophobic groups and can be used as the surface active agent, the dispersity, flame retardance, antibacterial property, antistatic property and other special properties can be given, and excellent surface activity and biocompatibility are achieved.

The invention discloses the method for preparation of nanometer gold ultramicron powders, comprising the following steps: 1 mixing the polyvinyl pyrrolidon solution and chlorauric acid solution; 2 adding natrium hydroxydatum solution; 3 heating with microwave, getting the nanometer gold sol; 4 evaporating it with vacuum rotatory evaporator and getting nanometer gold ultramicron powders. The method possesses the advantages of low cost, simple technology and wide applications.

The invention provides a carbon-based absorbing material, and a preparation method and an application thereof. The preparation method comprises the following steps: 1, weighing biomass carbon powder,ethyl cellulose and stearic acid according to a mass ratio of 1:(1-2):(1-2), wherein the biomass carbon powder is prepared through a preparation technology of the biomass carbon powder according to claim 1; 2, dissolving the biomass carbon powder and ethyl cellulose and stearic acid weighed in step 1 in an ethanol solvent to obtain a prepared solution; 3, heating and stirring the solution preparedin step 2 to obtain a stirred solution with a mass concentration of 8-16 g/L; and 4, placing a sponge in the stirred solution obtained in step 3 by using a dipping process to prepare the carbon-basedabsorbing material. The carbon-based absorbing material of the present invention can effectively absorb sunlight and improve the utilization efficiency of light.

The invention relates to a degradable zinc-based micropore drug-loaded support and a preparation method thereof. The support comprises a degradable zinc-based material support matrix, uniform drug-loaded micropores are arranged on the surface of the support matrix, a therapeutic medicine coating covers the surface provided with uniform drug-loaded micropores, and the support matrix is in a cylinder net structure. The preparation method comprises the following steps: 1)processing the degradable zinc based material to the support matrix; 2)pretreating the surface of the support matrix; 3)performing electrolytic polishing processing on the support matrix; 4)performing corrosion processing on the support matrix after polishing; 5)pickling the support matrix after corrosion; and 6)coating a therapeutic medicine on the surface of the support matrix after aperture making. The support has good support performance after implantation for more than 4 months due to superior corrosion resistance of a zinc alloy matrix material, uniform micropores are distributed on the matrix surface, the therapeutic medicine layer is coated, so that medicine release can be controlled, support effect of the metal support can be fully performed, and the drug-loaded support has medical science curative effect with drug eluting support for inhibiting intimal hyperplasia to restenosis.

The invention relates to a bacterial strain for fermentation of orange peel powder to produce a pectinase preparation and a method of enhancing enzyme activity by a surfactant. In the invention, Aspergillus japonicus PJ01 is employed to produce the pectinase preparation, the activity of the prepared pectinase preparation is represented by pectinase activity, cellulase activity and hemicellulase activity. The production method of the pectinase preparation includes: taking crushed orange peel powder as a sole carbon source, adding the orange peel powder into an inorganic nutrient salt in a Czapek medium, then adding 0.1-0.5%(wt) of PEG4000 into a liquid Aspergillus niger fermentation medium, carrying out shaking cultivation for 3-5 days, thus significantly improving the exo-pectinase activity, the cellulase activity (in terms of CMCase enzyme) and hemicellulase activity (in terms of Xylanase) relative to the control group. This method is simple, can significantly improve the pectinase preparation production ability of Aspergillus, and has the characteristic of environmental friendliness, thereby having good application prospects.

The invention discloses a nuclear-powered ship cable high-flame-resistance low-smoke halogen-free sheath material, and a preparation method thereof. The nuclear-powered ship cable high-flame-resistance low-smoke halogen-free sheath material comprises, by weight, 30 to 50 parts of ethylene-acrylate copolymer, 10 to 30 parts of polyester elastomer, 10 to 30 parts of nylon elastomer, 10 to 30 parts of polyethylene, 5 to 20 parts of a compatilizer, 150 to 200 parts of a fire retardant, 10 to 30 parts of a flame retardant synergist, 5 to 15 parts of a radiation resistant auxiliary agent, 1 to 3 parts of an anti-oxidant, 0.5 to 2.5 parts of a processing auxiliary agent, and 0.5 to 2.5 parts of an auxiliary crosslinking agent.

The invention discloses a method for detecting hydrogen peroxide by an electrochemical and fluorescent double-signal sensor based on Fe3O4@MnO2 and carbon dots. According to the invention, the good magnetism and electrochemical properties of a composite magnetic nano material Fe3O4@MnO2 are combined with fluorescence characteristics of a fluorescent nano material carbon dot off-on to construct anelectrochemical and fluorescent double-signal sensor based on a nano material, and the electrochemical and fluorescent double-signal sensor is applied to qualitative and quantitative detection of H2O2. The method of the double-signal sensor improves the sensitivity and selectivity of a single-signal sensor, can provide accurate and diverse analysis results, is simple and convenient to operate andhigh in universality, and can be applied to accurate detection of H2O2 in a biological sample.

The invention discloses a novel near-infrared high-quantum-yield dye which has the following structural formula I shown as the original specification, wherein R1, R2, R6 and R7 are hydrogen, a low-level alkyl, an ether group and a substituted alkyl or acyl that are independent respectively; R3, R4, R5, R8, R9 and R10 are hydrogen, a low-level alkyl or halogen that are independent respectively; R11 is hydrogen, methyl, a cyano group or trifluoromethyl; X<theta> is a negative ion. The invention discloses a preparation method of the dye. The dye has good biocompatibility, low toxicity, longer fluorescence-emission and high fluorescence quantum yield, the autofluorescence of a background is avoided, a fluorescence image with high signal-to-noise ratio can be obtained, and the dye is used for fluorescence labeling for nucleolar RNA in a biological system and plays an important pole in a study process of physiology related to the nucleolar RNA.

The invention discloses an ultra-thin composite film with graphene quantum dots as an intermediate layer and a preparation method of the composite film. The preparation steps of the ultra-thin composite film with the graphene quantum dots as the intermediate layer comprises a graphene quantum dot intermediate layer addition step, an interfacial polymerization reaction step, a chemical crosslinkingstep, and a solvent activating step. The separation performance of the film is significantly improved by adding the graphene quantum dot intermediate layer, the graphene quantum dots have a large amount of hydroxyl groups and carboxyl groups, the hydrophilicity of a base film can be increased, the pore size is reduced, the interfacial polymerization reaction is facilitated, and therefore the separation performance of the film is effectively improved. The preparation process is simple, and the composite film has a good application prospect in the field of organic solution system separation.

The present invention discloses a preparation method of bio-based bisphenol or polycarbonate material. The preparation method comprises: (1) adopting guaiacol or lilac phenol derivative as a raw material, and synthesizing bio-based bisphenol according to a stoichiometric ratio from the guaiacol or lilac phenol derivative and formaldehyde under catalysis of an acid; (2) dissolving the bio-based bisphenol monomer by using an alkali solution, adding a catalyst, adding a dichloromethane solution of triphosgene in a dropwise manner under vigorous stirring, and continuously carrying out a reaction for a certain time after completing the adding; and (3) stopping the reaction, layering the solution, washing the organic phase by using deionized water to achieve a neutral state, precipitating the product through an anti-solvent, and carrying out vacuum drying to obtain the bio-based polycarbonate material. According to the present invention, the prepared bio-based polycarbonate has good thermal stability; and the bio-based bisphenol is adopted as the raw material, and can replace the traditional petroleum-based bisphenol A so as to reduce dependence on the petrochemical resources, the advantages of environmental protection, low cost and simple and easy-performing process operation are provided, and the method is suitable for industrial production.

The present invention relates to the field of targeting drug delivery agents of polypeptide drugs, and particularly to a D-glutamyl-D-tryptophan sodium colon targeting delivery agent and a preparation method thereof. The colon targeting delivery agent comprises a tablet core, a pH value control layer, and a flora trigger layer. With the colon targeting drug delivery agent, the purpose of colon targeting drug delivery can be achieved, bioavailability of the D-glutamyl-D-tryptophan sodium is improved, and good mixing hardness and low toxicity of the agent of the present invention are provided compared with injection administration and nasal administration.

The invention relates to a UV (Ultraviolet) vacuum metalizing crackle coating, which comprises the following components: polyurethane acrylate, modified epoxy acrylate, thermoplastic acrylate, dipentaerythritol hexaacrylate, trimethylolpropane ethoxylate triacrylate, special tertiary amine acrylate, 1-hydroxy-cyclohexyl-benzyl ketone, butyl acetate, acetic ether, isobutyl alcohol and glycol ether. After the coating is cured through UV-irradiationUV irradiated so as to be cured, the surface of a coated parts shows a complex ice flower pattern effect instead of a boring mirror surface effect; the adhesive force of the coating is the same as that of the conventional coating, and the coating is resistant to boiling and synthetic perspiration; multiple experiments carried out on the components of the coating prove that the coating has better hypotoxicity compared with a traditional UV vacuum metalizing coating, is energy saving and environmental-ly friendly, simple and convenient to manufacture, and particularly suitable for a decorative effect of a cosmetics packaging structure and a special design of a panel for decoration.

The invention discloses a fluorescent carbon quantum dot based on biomass. The fluorescent carbon quantum dot is characterized in that raw materials of the fluorescent carbon quantum dot comprise thebiomass, and the biomass is selected from one or more of phaeophytin, arbutin and chestnut peptide. The fluorescent carbon quantum dot has an excitation peak in a near-infrared region, emits red fluorescence, can effectively generate singlet oxygen and overtemperature, and can be well used for near-infrared imaging and photodynamic and photothermal therapy. The invention also discloses a preparation method and application of the fluorescent carbon quantum dot.

The invention provides polyamino acid, which has a structure shown in a formula (I). Aminated methoxypolyethylene glycol is used as an initiator for initiating glycine-N-carboxyl anhydride, alanine-N-carboxyl anhydride, leucine-N-carboxyl anhydride or valine-N-carboxyl anhydride for polymerization, so that four polyamino acid-polyethylene glycol-polyamino acid triblock copolymers containing different side chain alkyl groups (H, CH3<->, (CH3)2CHCH2<->and (CH3)2CH<->) can be prepared. The application also provides drug-loaded gel which is prepared from the polyamino acid and an antitumor drug loaded on the polyamino acid. The drug-loaded gel provided by the application is prepared from the polyamino acid, thus having low toxicity and good temperature sensibility.

The invention provides polyester of a biological carbonic-ester-contained structure and a preparing method and application of the polyester. The structural formula of the polyester is shown in the description. The preparing method includes the steps of making one biological monomer in vanillic aldehyde, syringaldehyde and p-hydroxy benzaldehyde serve as the raw material to react with triphosgene under the effect of a catalyst, recrystallizing a reaction product to obtain a dialdehyde monomer of a biological carbonic-ester-contained structure, making the dialdehyde monomer serve as the raw material to react with sodium borohydride, conducting recrystallizing or column-passing separating on a reaction product to obtain a diol polymerizable monomer of a biological carbonic-ester-contained structure, making the diol polymerizable monomer react with acyl chloride under the effect of a catalyst, dropping a reaction product in an anti-solvent for regeneration, and conducting washing and purifying to obtain the polyester of the biological carbonic-ester-contained structure. The polyester is of the biological carbonic-ester-contained structure and is friendly to the environment; the preparing method of the polyester is simple, no harmful substances are generated in production, pollution is low, and cost is low.

The invention discloses membrane permeability dye with a large two-photon fluorescence active cross section. The dye adopts triphenylamine heterocyclic chemical compounds, and the chemical structure of the dye is shown in the formula (I). The invention further discloses an application of the dye in displaying two-photon imaging of cytoplasm in a living cell. Experiments show that the dye has characteristics of larger two-photon fluorescence active absorption cross section, excellent cell membrane permeability, low toxicity and the like, also has the characteristics of wide application range, low price and good bio-compatibility with known probe DAPI (4',6-diamidino-2-phenylindole) and has wide application prospect in the field of laser excitation fluorescence biomarkers.

The invention discloses a preparation method of a PVA film used for a polaroid. The preparation method comprises the steps of mixing PVA power with a plasticizer, then uniformly mixing water-soluble graphene quantum dots into the mixture, pouring the mixture of the three materials into a mixed solvent composed of methanol and distilled water, mixing, dissolving and stirring the mixture at a temperature of 70-90 DEG C for 6-12h to form a mixed solution, subjecting the mixed solution to standing, defoaming and film formation, and carrying out water washing and swelling, dyeing, extension and correction on the mixed solution to obtain polarized properties. The invention further discloses a polaroid, which is prepared by laminating the PVA film doped with the graphene quantum dots, a TAC film, a protective film and an pressure sensitive adhesive; and the addition of the graphene quantum dots can effectively prevent a ''blue leakage'' phenomenon, and improve the heat and humidity resistance of the polaroid.

The invention relates to a specific fluorescence labeling method of food-borne probiotics. The method includes following steps: (a), preparing a Cr3+doped gallium zinc germanate long-afterglow fluorescent nano probe having near infrared fluorescence and ultralong-afterglow properties through a solvothermal-high-temperature calcining process; (b), realizing specific fluorescence labeling of the nano probe to target probiotics through antibody surface functionalizing. The method has the advantages that the nano probe prepared by the method has ultrastrong near infrared luminescence, ultralong afterglow life, excellent biocompatibility and structural stability, high grain size uniformity and low toxicity; near infrared fluorescence labeling probiotics in-vivo biological imaging technology developed by the invention can realize nondestructive tracing of distribution of probiotics after entering organisms, and the method is of important significance in developing functional sites of innovative food-borne probiotics and nutriology research concepts.