5results about How to "Good low toxicity" patented technology

The invention discloses a puerarin nanobelt with stable homologous polysaccharide and a preparation method of the puerarin nanobelt, and belongs to the technical field of medicine nanobelts. The puerarin nanobelt is prepared by taking puerarin and pueraria polysaccharide as raw materials through a medium grinding method. The preparation method comprises the following steps: weighing a puerarin raw material medicine and a puerarin polysaccharide raw material medicine; adding the kudzuvine root polysaccharide into water until the kudzuvine root polysaccharide is completely dissolved; the preparation method comprises the following steps: adding water into a penicillin bottle, adding puerarin, adding a stirrer and 0.4-0.6 mm zirconium oxide beads with the same volume as water, putting the penicillin bottle on a magnetic stirrer, grinding at a magnetic stirring speed of 800-1400 rpm for 2-12 hours, taking out, and filtering to remove the zirconium oxide beads, thereby obtaining the puerarin nanobelt. The preparation method has the advantages that the polysaccharide stabilizer with a natural source is adopted, has the characteristics of good biocompatibility, low toxicity, biodegradability and the like, and shows remarkable advantages in a nano delivery system; the accumulative dissolution amount is greatly increased, and the balance time is greatly shortened; the oral absorption of puerarin can be obviously improved.

The present application relates to the technical field of microbiology and animal vaccine cross, and particularly relates to a gene deletion attenuated mutant of wild strain of Pseudomonas plecoglossicida, a construction method and application thereof, and provides the gene deletion attenuated mutant based on deletion of the coding genes of proteins participating in maintaining cell membrane lipid asymmetry of Pseudomonas plecoglossicida strain on the wild strain of Pseudomonas plecoglossicida mlaE , the coding gene of response regulator protein of envZ / ompR two-component system ompR , and the coding gene of DeoR family transcriptional regulator deoR The mutant strain has obvious low toxicity, can effectively protect the test fish from the invasion of Pseudomonas plecoglossicida causing the disease of Pseudomonas plecoglossicida visceral white spot disease, and researches show that the vaccine prepared based on the gene deletion attenuated mutant has significant immunization effect and excellent prevention and treatment effect on the disease of Pseudomonas plecoglossicida visceral white spot disease in aquaculture.

The application provides a sealant and a preparation method thereof, and relates to the technical field of sealants. The sealant comprises A component and B component. The A component comprises the following raw materials: bio-based polyaspartic ester resin, modified nano zinc oxide, tea polyphenol microcapsule, filler, dispersant and defoaming agent. The B component comprises the following raw materials: bio-based isocyanate prepolymer, HDI trimer, catalyst and light stabilizer. The sealant provided by the application has the comprehensive performances of weather resistance, anti-yellowing, mechanical balance, environmental protection, safety, antibiosis and durability, and is suitable for the sealing of building gaps in the environment exposed to light and moisture for a long time.

The invention relates to the field of plant external vesicles, in particular to a ginseng external vesicle composition loaded with quercetin as well as a preparation method and application thereof, and the preparation method comprises the following steps: preparing ginseng external vesicle supernate; the preparation method comprises the following steps: dissolving quercetin in dimethyl sulfoxide to obtain a quercetin solution; mixing the quercetin solution with the ginseng outer vesicle supernate, and incubating at room temperature for 1-2 hours; and centrifuging and carrying out solid-liquid separation to obtain a precipitate, namely the composition. The quercetin is loaded by using the extracellular vesicles, so that ROS generation can be reduced, the activity of SOD in cells can be improved, and the MDA content can be reduced. The activity of key enzyme in a cell anti-oxidation system is enhanced, so that damage caused by oxidative stress is relieved, and a certain repairing effect is achieved.

The invention provides a DNA tetrahedron drug compound for treating retinal ischemia reperfusion injury, and belongs to the field of eye disease treatment and molecular biology, and the tetrahedron drug compound is connected with YAP saRNA. The tetrahedral drug compound can efficiently and safely deliver YAP saRNA, improve the expression level of YAP in cells and inhibit oxidative stress and ferroptosis of retinal cells, so that RI / RI is effectively treated, and a new choice is provided for treating the tetrahedral drug compound.