30 results about "Pseudomonas plecoglossicida" patented technology

The invention discloses pseudomonas plecoglossicida and application thereof and particularly provides pseudomonas plecoglossicida BD with the preservation number being CGMCC No.14205. The provided pseudomonas plecoglossicida BD can grow and propagate with nitrile compounds as the only energy source, can degrade contaminants such as the nitrile compounds in acrylonitrile waste water and reduces the chemical oxygen demand and the biochemical oxygen demand of the acrylonitrile waste water, and accordingly the biodegradability of the acrylonitrile waste water is improved.

The invention provides a Pseudomonas plecoglossicida fliA gene silenced strain that is Pseudomonas plecoglossicida fliA-RNAi and deposited in the China Center for Type Culture Collection on November 12th, 2018 with an accession number of CCTCC NO:M 2018770. The constructed Pseudomonas plecoglossicida fliA gene silenced strain has virulence significantly lower than that of a Pseudomonas plecoglossicida wild strain, time of death caused by same infection conditions is delayed, and the death rate is greatly reduced, thus laying a theoretical foundation for development of an attenuated Pseudomonasplecoglossicida vaccine.

The invention discloses a method for analyzing a root domain soil microbial community structure of a soft-rot diseased amorphophallus konjac plant. The method comprises the following steps: acquiring and treating a test sample, preparing a culture medium, performing microorganism isolation and enumeration, identifying dominant strains, testing the pH and the nutrition of soil, and processing data. By adopting the method for analyzing the root domain soil microbial community structure of the soft-rot diseased amorphophallus konjac plant, the change of the soil microbial community structure with the amorphophallus konjac soft-rot disease is studied, relevant micro-ecology mechanisms of the amorphophallus konjac soft-rot disease can be studied for a first time, and great significance is achieved. The study of the method for analyzing the root domain soil microbial community structure of the soft-rot diseased amorphophallus konjac plant shows that 9 dominant microorganisms, namely, 3 dominant bacteria including pseudomonas plecoglossicida, pseudomonas chlororaphis subsp.aureofaciens and stenotrophomonas pavanii, 4 dominant bacteria including fusarium solani, fusarium oxysporum, penicillium canescens and penucillium, and 2 dominant actionmycetes including streptomyces scabiei and streptomyces cellulosae, massively exist at the root domain of the soft-rot diseased amorphophallus konjac plant.

The present invention relates to a phenol-degrading bacterium strain capable of producing a biological surfactant and an application thereof. A technical solution is as follows: the provided bacteriumstrain is pseudomonas plecoglossicida BPH-3 which is preserved by the China Center for Type Culture Collection, a preservation number is: CCTCC NO:M 2019144, and a preservation date is March 14, 2019. A GenBank accession number of 16S rDNA of the pseudomonas plecoglossicida BPH-3 is MK575471, the pseudomonas plecoglossicida BPH-3 is a Gram-negative bacterium strain, and colony is milky white andround, smooth in surface and neat in edges; and after Gram staining, the pseudomonas plecoglossicida BPH-3 is negative under a microscope and presents a short-rod shape. During fermentation degradation, the phenol-degrading bacterium strain forms a glycolipid biological surfactant which can promote solubilization, emulsification, wetting and osmosis of long-chain alkanes and can effectively degrade phenol in coking wastewater and petrochemical wastewater.

The invention provides a real-time fluorescence quantitative PCR detection kit for a deformed pseudomonas plecoglossicida TaqMan and a preparation method thereof. The kit comprises a primer and a probe for specifically detecting the deformed pseudomonas plecoglossicida, wherein the sequence is as follows: a formula as shown in the specification. The kit provided by the invention is complete in reagents and simple to operate, and provides a detection tool for the scale detection and control of the disease.

The invention discloses a pseudomonas plecoglossicida DNA vaccine and a preparing method and application thereof. The pseudomonas plecoglossicida DNA vaccine is characterized in that the pseudomonas plecoglossicida DNA vaccine is pCI-pcrV; pseudomonas plecoglossicida NB2011 genome DNA serves as a template, and is subjected to PCR amplification through a primer P1/P2, the amplified products are purified to be connected with plasmid pCI-neo, and recombinant plasmid pCI-pcrV is obtained, and is the pseudomonas plecoglossicida DNA vaccine, wherein the sequence of a target gene pcrV is shown in SEQID NO:1. According to the pseudomonas plecoglossicida DNA vaccine, the immune protective rate of pseudomonas plecoglossicida infection is 70% or above, and therefore the high protective rate is achieved. The immune protective rate of the pseudomonas plecoglossicida DNA vaccine in 8 weeks after immunization is not obviously reduced, and therefore the pseudomonas plecoglossicida DNA vaccine has thelong-acting advantage.

The invention relates to aptamers and particularly relates to Pseudomonas plecoglossicida aptamers and a screening method therefor. Sequences of the aptamers are represented by SEQ. NO. 1-5, the aptamers capable of specifically identifying and inhibiting the growth of Pseudomonas plecoglossicida are screened by employing SELEX technologies such as inverse screening, and the influence on the Pseudomonas plecoglossicida caused by the aptamers is characterized through measuring affinity. According to the Pseudomonas plecoglossicida aptamers and the screening method therefor, high-frequency sequences can be objectively and effectively classified, and thus, an objective and effective basis is provided for subsequent accurate selection and verification on the aptamers; and the method is simple and is convenient in operation, so that the blindness of verification is lowered greatly, and the screening efficiency is increased. Five aptamers #1, #2, #3, #4 and #5 with highest relative important indexes are picked from a screened result, the affinity of these aptamers to 28-DEG-C Pseudomonas plecoglossicida is higher than that of other bacteria, and thus, these aptamers have relatively good affinity and specificity to the 28-DEG-C Pseudomonas plecoglossicida.

The invention relates to a traditional Chinese medicine composition used for treating grouper pseudomonas plecoglossicida and a preparation method and an application thereof, which belong to the technical field of aquatic product culture. The traditional Chinese medicine composition comprises coptis, radix astragali and phellodendron amurense with mass ratio being 0.9-1.3:0.9-1.3:0.9-1.3. The traditional Chinese medicine composition can greatly increase the drug effect, and the survival rate by using the traditional Chinese medicine composition is increased by 60% and above by comparing with that of non-feeding of the traditional Chinese medicine composition. The traditional Chinese medicine composition has the advantages of scientific compatibility, low cost, short treatment course, fasteffectiveness, and high cure rate, and provides support for preventing and treating fish disease in culture production.

The invention discloses a Pseudomonas plecoglossicida H tpG antigen and a preparation method of a polyclonal antibody of the Pseudomonas plecoglossicida H tpG antigen. The method includes: the following steps: firstly, artificially synthesizing an H tpG gene according to codon preference of escherichia coli, inserting the H tpG gene into a pET-32 expression vector, and selecting positive clone for sequencing verification; then, transforming the constructed plasmid into BL21DE3 competent cells, performing culturing, inducing, splitting and centrifuging to obtain protein, and detecting the expression condition of the protein by SDS-PAGE; further, selecting a strain with good sample expression for inoculation, culture, induction, splitting decomposition and purification, obtaining protein, and identifying the molecular weight, purity and concentration of the protein through SDS-PAGE; furthermore, taking the obtained H tpG protein as an antigen for animal immunization, collecting and purifying antiserum, and obtaining the H tpG antibody. The HtpG antigen of pseudomonas proteinans is obtained, and the corresponding HtpG polyclonal antibody is prepared. The titer of the antiserum and the affinity purification antibody is detected by an indirect ELISA method, and the result shows that the yield of the affinity purification antibody is normal. The method is simple, and the prepared antibody is high in immunogenicity.