21 results about "Pseudomonas plecoglossicida" patented technology

The invention discloses Klebsiella oxytoca G1-2-4 and application thereof, and particularly relates to application of the Klebsiella oxytoca G1-2-4 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.30986 in preparation of a biocontrol preparation or feed for preventing and treating aquatic diseases. The invention further discloses application of the Klebsiella oxytoca G1-2-4 in preparation of the biocontrol preparation or feed for preventing and treating the aquatic diseases and application of the Klebsiella oxytoca G1-2-4 in preparation of the biocontrol preparation or feed for preventing and treating the aquatic diseases. The invention relates to an application of klebsiella oxytoca G1-2-4 in inhibiting the growth of pseudomonas plecoglossicida and a biocontrol preparation or feed containing the klebsiella oxytoca G1-2-4 and used for preventing and treating aquatic diseases. According to the Klebsiella oxytoca cultured in an isolated mode, the disease resistance of the Klebsiella oxytoca is verified through in-vitro and in-vivo experiments, the disease resistance mechanism of the Klebsiella oxytoca is preliminarily explored, and reference is provided for research on prevention and treatment of the visceral white-spot disease of the small yellow croakers.

The invention provides a fish source pseudomonas plecoglossicida, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 2024372. The invention also relates to an application of the fish-derived pseudomonas plecoglossicida in preparation of a vaccine for preventing fish visceral white-spot disease. The invention also relates to a vaccine for preventing the fish visceral white-spot disease, which is characterized by comprising the inactivated fish source pseudomonas plecoglossicida. According to the invention, the growth characteristics and toxicity of the separated Pseudomonas plecoglossicida (ND2304 strain) are determined, and the Pseudomonas plecoglossicida (ND2304 strain) is determined to be a virulent strain with strong pathogenicity to large yellow croaker; the prepared corresponding Pseudomonas plecoglossicida inactivated vaccine can be combined with an adjuvant, and the inactivated vaccine is relatively high in safety and relatively high in relative protection rate.

The invention relates to the technical field of bacterial detection, and discloses a composition and a method for detecting pseudomonas plecoglossicida based on CRISPR / Cas12a (Clustered Regularly Interspaced Short Palindromic Repeats / Cas12a). The composition comprises a primer group, crRNA (Complementary Ribonucleic Acid) and Cas12a protein, and the crRNA comprises a sequence as shown in SEQ ID NO. 3. When the composition disclosed by the invention is used for detecting the pseudomonas plecoglossicida, relatively high sensitivity and specificity can be realized.

The application of microbial agent and biochar in the prevention and treatment of tomato root-knot nematode disease is disclosed, which relates to the technical field of tomato root-knot nematode prevention and treatment. The microbial agent is Pseudomonas plecoglossicida, the dosage of Pseudomonas plecoglossicida is 2 kg per mu, and the application amount of biochar is 1 t per mu. Pseudomonas plecoglossicida is dripped into water by drip irrigation, and is dripped once every 20 days. Biochar is applied as base fertilizer before tomato planting. After data measurement, arrangement and analysis, it is found that in the greenhouse soil infected with root-knot nematode disease in Hexi region of Gansu province, when the dosage of Pseudomonas plecoglossicida is 2 kg per mu, and the application amount of biochar is 1 t per mu, the tomato grows well under the condition, the quality is better, and the root-knot nematode disease has better control effect.

The invention relates to a microbial agent containing pseudomonas deformation and ochrobactrum intermedium and application of the microbial agent. The microbial agent comprises the pseudomonas deformation and the ochrobactrum intermedium. The pseudomonas mutans is classified and named as pseudomonas mutans, and the preservation number of the pseudomonas mutans is CGMCC (China General Microbiological Culture Collection Center) No.14475; the classification name of the ochrobactrum intermedium is Ochrobactrum intermedium, and the preservation number of the ochrobactrum intermedium is CGMCC (China General Microbiological Culture Collection Center) No.33645. The invention further discloses a preparation method of the ochrobactrum intermedium. The microbial agent disclosed by the invention can be used for efficiently, stably and environmentally-friendly biological control of plant diseases.

The application discloses a kind of anti-saline-alkali microbial inoculants and preparation method thereof, belong to the field of microbial technology.The microbial inoculants is made of composite microbial inoculants 100-150 parts, fish bone hydrolysate 10-30 parts, nano silicon sol 1-3 parts.The composite microbial inoculants is made of Trichoderma reesei, Pseudomonas plecoglossicida, Acinetobacter baumannii.The anti-saline-alkali microbial inoculants has remarkable effect on saline-alkali soil improvement, greatly reduces soil acidity, salt content and bulk density, and the yield of saline-alkali soil wheat planting is obviously improved.The soil organic matter content and soil enzyme activity are significantly improved after use, the soil quality and rhizosphere microecological environment are improved, and the enzyme activity in wheat is improved, the stress resistance is improved, the yield reduction caused by saline-alkali stress is reduced, and the use of microbial inoculants can also reduce the amount of chemical fertilizer and pesticide, reduce production cost, and further improve the economic benefit of wheat planting.

The invention discloses staphylococcus epidermidis 2251 and application of the staphylococcus epidermidis 2251, the preservation number of the staphylococcus epidermidis 2251 is CGMCC (China General Microbiological Culture Collection Center) NO.30987, and the staphylococcus epidermidis 2251 is applied to preparation of a biocontrol preparation or feed for preventing and treating aquatic diseases. The invention relates to application of staphylococcus epidermidis 2251 in inhibiting growth of pseudomonas plecoglossicida and a biocontrol preparation or feed containing the staphylococcus epidermidis 2251 and used for preventing and treating aquatic diseases. According to the staphylococcus epidermidis subjected to isolated culture, the disease resistance of the staphylococcus epidermidis is verified through in-vitro and in-vivo experiments, the disease resistance mechanism of the staphylococcus epidermidis is preliminarily explored, and reference is provided for prevention and treatment research on the visceral white-spot disease of the small yellow croakers.

The application provides a newly isolated Pseudomonas plecoglossicida phage, a preparation method and application thereof. The phage is preserved in the China Center for Type Culture Collection on February 15, 2023, and the preservation number is CCTCC NO:M 2023124. The application discloses a phage which can efficiently inhibit the growth of Pseudomonas plecoglossicida, and can only infect Pseudomonas plecoglossicida, and has high specificity. The phage can be applied to the fields of agriculture, medicine, environment and the like to resist the bacterial host, and has a good development prospect as an antibiotic substitute.

The present invention discloses Staphylococcus epidermidis 2251 and its applications. Staphylococcus epidermidis 2251 has a preservation number of CGMCC NO. 30987. The applications of Staphylococcus epidermidis 2251 in preparing a biocontrol preparation or feed for preventing and treating aquatic diseases; the application of Staphylococcus epidermidis 2251 in inhibiting the growth of Pseudomonas plecoglossicida, and a biocontrol preparation or feed for preventing and treating aquatic diseases containing Staphylococcus epidermidis 2251. A strain of Staphylococcus epidermidis isolated and cultured in the present invention has verified the disease resistance of the strain through in vitro and in vivo experiments, and preliminarily explored its disease resistance mechanism, providing a reference for the prevention and treatment research of the visceral white spot disease of small yellow croaker.

This invention provides a *Pseudomonas plecoglossicida* strain, its inoculant, and its applications, relating to the field of microbiology. Specifically, the *Pseudomonas plecoglossicida* NT strain, with accession number CGMCC No. 25090, can efficiently convert nitrate nitrogen, reduce the accumulation of nitrates in soil, and remediate secondary salinized soils. It also possesses the ability to produce extracellular polysaccharides, promoting the formation of soil aggregates and increasing soil cohesion. Furthermore, this strain can successfully colonize the rhizosphere soil of vegetables, improving the rhizosphere ecological environment, promoting vegetable growth, and increasing yield. This achieves the goal of ensuring both the sustainable development of modern agriculture and the quality and quantity of crops. The strain of this invention has relatively comprehensive soil remediation capabilities and has enormous application potential.

The use of allicin E for controlling white-spot disease in Larimichthys crocea, which use can effectively reduce the mortality of Larimichthys crocea, alleviate pathological changes in the spleen and liver of Larimichthys crocea caused by Pseudomonas plecoglossicida, and reduce the threats to food and environmental safety caused by the long-term use of antibiotics in Larimichthys crocea.

The invention relates to a primer pair, a composition, a kit and a method for detecting pseudomonas plecoglossicida causing large yellow croaker visceral white-spot disease based on an RPA-CRISPR / Cas12a technology, the nucleotide sequences of upstream primers RPA-F are respectively as shown in SEQ ID NO.1-4, the nucleotide sequences of downstream primers RPA-R corresponding to the upstream primers RPA-F are respectively as shown in SEQ ID NO.5-8, and the composition comprises the primer pair, crRNA, Cas12a protein and a fluorescent reporter probe. Compared with the prior art, the invention establishes a P. plecoglossicida detection method which is simple and convenient to operate, short in detection time, good in specificity and high in sensitivity and only needs common constant-temperature equipment, and is suitable for on-site rapid detection in basic laboratories and large yellow croaker farms.

The invention discloses an application of Harman alkali in the aspect of preparing a Pseudomonas plecoglossicida inhibitor, and is characterized in that the Harman alkali is applied in the aspect of preparing a medicine for preventing and / or treating aquatic animal infectious diseases caused by Pseudomonas plecoglossicida. The invention further provides application of the harman alkali or the pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and treating aquatic animal plecoglossus altivelis pseudomonas infection, application of the harman alkali or the pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and treating plecoglossus altivelis liver injury, application of a plecoglossus altivelis immunoregulation medicine and application of the harman alkali or the pharmaceutically acceptable salt thereof in preparation of an aquaculture feed additive. The method has the advantages that the survival rate of aquatic animals can be remarkably increased in a Pseudomonas plecoglossicida infection model, the bacterial load in immune tissues such as blood, liver, spleen, head and kidney and the like is reduced, and liver and spleen tissue pathological injury and cell apoptosis caused by infection are relieved; meanwhile, harmine can improve biochemical and antioxidant indexes of infection-related serum, and has no obvious toxicity to fish-derived cells within a certain concentration range.

The invention belongs to the technical field of microorganisms, and particularly relates to drought-enduring phosphorus-solubilizing pantoea CYIJM06 and application thereof. The invention provides pantoea CYIJM06, the preservation number is CCTCC NO: M 20222103, the pantoea CYIJM06 is drought-resistant and strong in phosphate solubilizing capacity, and the pantoea CYIJM06 is prepared into a fungicide for application and can improve soil, activate soil phosphorus and increase the content of available phosphorus in the soil. The Pantoea sp. CYIJM06 provided by the invention is especially suitable for activation of phosphorus in soil in arid and / or semi-arid regions, the content of rapidly available phosphorus in the soil in the arid and / or semi-arid regions can be remarkably increased, and application of phosphate fertilizer is reduced. The phosphorus-solubilizing strain provided by the invention not only can release micromolecular activated fixed inorganic phosphorus such as organic acid in a culture medium, but also can secrete phosphatase in the culture medium to hydrolyze organic phosphorus, so that the phosphorus activation of soil is obviously promoted.

The invention discloses a pseudomonas plecoglossicida recombinant strain for expressing green fluorescence as well as a construction method and application of the pseudomonas plecoglossicida recombinant strain. The construction method comprises the following steps: constructing a pBBR1-GFP plasmid, and transforming the pBBR1-GFP plasmid into pseudomonas plecoglossa NB2011, so as to obtain a recombinant strain P. plecoglossa NB2011 / pBBR1-GFP capable of stably expressing green fluorescence. The recombinant strain shows green fluorescence when being observed under a fluorescence microscope, the plasmid stability rate can reach 100% after 30 generations of passage, the strain is constructed through an electrotransformation method, positive clones are screened through chloramphenicol, the strain has high hereditary stability, and through an infection experiment, the strain has good clinical application prospects. The fluorescent recombinant strain shows good visualization ability in mouse macrophages J774A.1 and juvenile zebrafish, and the interaction between the strain and host cells and tissues can be observed in real time. The invention provides a visual and stable fluorescence labeling tool, can be used for researching the pathogenic mechanism of pseudomonas plecoglossicida, and provides a new method and technical means for researching the interaction between pathogenic bacteria and hosts.

This invention discloses a bacterium that degrades autotoxic substances and its applications. The specific bacterium providing this invention is *Pseudomonas plecoglossicida*, strain number 56-21, with accession number CGMCC No. 26763 at the China General Microbiological Culture Collection Center. *Pseudomonas plecoglossicida* 56-21 (CGMCC No. 26763) provided by this invention can utilize p-hydroxybenzoic acid or vanillin as the sole carbon source for growth and can degrade autotoxic substances such as p-hydroxybenzoic acid or vanillin, which is of great significance for alleviating continuous cropping obstacles and improving the soil environment.

The invention provides a specific primer group for detecting Pseudomonas plecoglossicida, which comprises specific primers, and the specific primers comprise an upstream primer P.p-RPA-F, a downstream primer P.p-RPA-F, an upstream primer P.p-RPA-F, a downstream primer P.p-RPA-F, a downstream primer P.p-RPA-F, a downstream primer P.p-RPA-F and a downstream primer P.p-RPA-F, and the sequence of the downstream primer P.p-RPA-R is as shown in SEQ ID NO: 2. The invention further provides a corresponding kit, application and a method. The specific primer group and the kit are suitable for rapid detection of the visceral white-spot disease of the pseudosciaena crocea in a basic laboratory and a pseudosciaena crocea farm site, the rapid propagation and diffusion risk of the new pathogen is reduced, the prevention and control effect on the visceral white-spot disease is improved, and a technical support is provided for detection and disease diagnosis of the visceral white-spot disease of the pseudosciaena crocea.

This invention discloses a strain of *Pseudomonas proteus* and its applications, belonging to the field of microbial technology. The *Pseudomonas proteus* strain is named *Pseudomonas proteus* (…). Pseudomonas plecoglossicida Sg308, deposited on March 1, 2026, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 37822, significantly enhances the insecticidal activity of Bacillus thuringiensis against the Asian corn borer. Its mechanism of action primarily involves entry into the hemocoel through perforations caused by Bacillus thuringiensis, leading to septicemia and thus enhancing the lethal effect of Bt toxin. The combined use of this strain and Bt can significantly improve control efficacy and reduce pesticide usage, showing broad application prospects in the green control of the Asian corn borer.

The invention relates to a lytic pantoea agglomerans phage and application thereof, and belongs to the technical field of bioengineering. The lytic pantoea agglomerans phage is preserved in the China Center for Type Culture Collection on October 30, 2025, the preservation address is Wuhan, China, and the preservation number is CCTCC NO: M 20252393; the strain is named as Pantoea agglomerans phage vBPanPgDPHH-6 in a classified manner, and the strain is named as Pantoea agglomerans phage The bacteriophage provides a brand new way for solving the problem of drug resistance of pantoea agglomerans, even if pathogenic bacteria generate resistance to conventional chemical pesticides or antibiotics, as long as the characteristics of the pathogenic bacteria serving as bacteriophage hosts are not changed, the bacteriophage can still efficiently split the pathogenic bacteria, and reliable guarantee is provided for preventing and controlling infection caused by drug-resistant strains.

The invention relates to a condition deletion mutant strain of Pseudomonas plecoglossicida phosphoric acid pantetheinyl transferase and application thereof, starting from a wild strain P.plecoglossicida NB2011, firstly, a recombinant plasmid pET22b-entD integrated with an escherichia coli entD (PPTase participating in siderophore enterobacter synthesis) gene sequence is transformed into the NB2011, under the condition, a double homologous recombination method is adopted, a primer is designed, and a mutant strain of Pseudomonas plecoglossicida phosphoric acid pantetheinyl transferase is obtained. The method comprises the following steps: by taking an NB2011 PPTase coding sequence as a target gene, constructing a deletion mutant strain delta PPT / pET22b-entD NB2011 in a PPTase frame; the growth rate, siderophore generation and other biological phenotypes of the wild strain and the mutant strain are analyzed, and by testing the adhesion, internalization and proliferation characteristics of the deletion strain to mouse macrophage J774A.1 and the virulence change of the deletion strain to large yellow croaker, the influence of PPTase on the thallus virulence is evaluated; according to the invention, the successful construction of the condition deletion mutant strain can provide a necessary strain basis for in-depth study on the biological function of the PPTase in the P.plecoglossicida and the effect of the PPTase in a pathogenic mechanism.

The invention relates to a rapid vibrio parahaemolyticus detection kit based on a one-tube method RPA-CRISPR / Cas12a and a detection method of the rapid vibrio parahaemolyticus detection kit. A specific RPA (recombinase polymerase amplification) primer and CRISPR crRNA (clustered regularly interspaced short palindromic repeats) are designed by taking a ToxR gene of vibrio parahaemolyticus as According to the established one-tube method RPA-CRISPR / Cas12a detection system, the risk of aerosol pollution caused by a cover opening process is effectively avoided. The method disclosed by the invention is high in specificity, and has no cross reaction with related vibrios such as vibrio vulnificus, vibrio alginolyticus, vibrio cholerae, vibrio harveyi and vibrio anguillarum, and other aquatic pathogenic bacteria such as pseudomonas aeruginosa and pseudomonas plecoglossicida; the sensitivity is high, and the lowest detection limit of genome DNA is as low as 1 pg / mu L; the detection is rapid, and the whole process of nucleic acid amplification and fluorescence detection only needs about 30 minutes. The kit and the method do not need complex instruments and equipment, are simple to operate, are suitable for on-site rapid detection of aquatic product samples in a base layer, and have important application values in early screening, epidemiological investigation and comprehensive prevention and control of vibrio parahaemolyticus.