30 results about "Pseudomonas plecoglossicida" patented technology

The invention provides a Pseudomonas plecoglossicida fliA gene silenced strain that is Pseudomonas plecoglossicida fliA-RNAi and deposited in the China Center for Type Culture Collection on November 12th, 2018 with an accession number of CCTCC NO:M 2018770. The constructed Pseudomonas plecoglossicida fliA gene silenced strain has virulence significantly lower than that of a Pseudomonas plecoglossicida wild strain, time of death caused by same infection conditions is delayed, and the death rate is greatly reduced, thus laying a theoretical foundation for development of an attenuated Pseudomonasplecoglossicida vaccine.

The invention discloses a Pseudomonas plecoglossicida and an application thereof, and specifically provides a Pseudomonas plecoglossicida BD, whose preservation number is CGMCC No.14205. The embodiment of the present invention provides Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BD, which can grow and reproduce with nitrile compounds as the only energy source, can degrade pollutants such as nitrile compounds in acrylonitrile wastewater, and reduce the chemical oxygen demand of acrylonitrile wastewater Amount and biochemical oxygen demand, thereby improving the biodegradability of acrylonitrile wastewater.

The invention discloses a pseudomonas plecoglossicida DNA vaccine and a preparing method and application thereof. The pseudomonas plecoglossicida DNA vaccine is characterized in that the pseudomonas plecoglossicida DNA vaccine is pCI-pcrV; pseudomonas plecoglossicida NB2011 genome DNA serves as a template, and is subjected to PCR amplification through a primer P1 / P2, the amplified products are purified to be connected with plasmid pCI-neo, and recombinant plasmid pCI-pcrV is obtained, and is the pseudomonas plecoglossicida DNA vaccine, wherein the sequence of a target gene pcrV is shown in SEQID NO:1. According to the pseudomonas plecoglossicida DNA vaccine, the immune protective rate of pseudomonas plecoglossicida infection is 70% or above, and therefore the high protective rate is achieved. The immune protective rate of the pseudomonas plecoglossicida DNA vaccine in 8 weeks after immunization is not obviously reduced, and therefore the pseudomonas plecoglossicida DNA vaccine has thelong-acting advantage.

The present invention relates to a phenol-degrading bacterium producing biological surfactant and its application. Its technical scheme is: the provided bacterial strain is Pseudomonas plecoglossicida BPH-3, which is preserved by the China Center for Type Culture Collection, and the preservation number is: CCTCC NO: M 2019144, and the preservation date is March 14, 2019 , the GenBank accession number of the 16S rDNA of the Pseudomonas plecoglossicida (Pseudomonas plecoglossicida) BPH-3 is MK575471, and the Pseudomonas (Pseudomonas plecoglossicida) BPH-3 is a gram-negative bacteria, and the colony is milky white, circular , smooth surface, neat edges; negative under the microscope after Gram staining, the strain was short rod-shaped. The phenol-degrading bacteria produced a glycolipid biosurfactant in the process of fermentation and degradation, which can promote the solubilization, emulsification, wetting and penetration of long-chain alkanes, and can effectively degrade phenol in coking wastewater and petrochemical wastewater.

The present invention discloses a multiple PCR detection kit for rapidly identifying pathogenic Pseudomonas plecoglossicida, and a method thereof. The kit comprises TaqDNA polymerase, a PCR buffer, a deoxyribonucleoside triphosphate mixture, double distilled water, and primer pairs of Pseudomonadaceae, Pseudomonas plecoglossicida and pathogenic Pseudomonas plecoglossicida. The multiple PCR detection method for rapidly identifying pathogenic Pseudomonas plecoglossicida by using the kit comprises: primary separation of Pseudomonadaceae, and multiple PCR amplifications. Compared with the kit and the method in the prior art, the kit and the method of the present invention have the following advantages that: with application of the kit of the present invention to detect, Pseudomonadaceae, Pseudomonas plecoglossicida and pathogenic Pseudomonas plecoglossicida corresponding to the DNA primer pairs of the three Pseudomonadaceae can be rapidly detected, and with the detection method of the present invention, the presence of the Pseudomonadaceae can be rapidly, sensitively and specifically determined by using the kit, and the pathogenic stain and the non-virulent environment isolation strain can be distinguished.