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Phenol-degrading bacterium strain capable of producing biological surfactant and application thereof

A technology of phenol-degrading bacteria and surfactants, applied in the field of phenol-degrading bacteria, can solve the problems of increasing the production cost of biosurfactants, harsh conditions of biosurfactants, strains that cannot adapt to the solution environment of phenols, etc., and achieve good degradation effect, strong emulsification, effect of promoting solubilization

Active Publication Date: 2019-06-04
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the patented technology of "a method for producing biosurfactant by a strain of Pseudomonas" (CN102732573A), the advantage of this technology is that the rate of biosurfactant production is relatively fast, but the strain itself cannot adapt to the solution environment containing phenols; " A strain of Pseudomonas aeruginosa that produces biosurfactants" (CN101705200A) patented technology, the advantage of this technology is high yield, but the conditions for producing biosurfactants are relatively harsh, which increases the production cost of biosurfactants

Method used

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  • Phenol-degrading bacterium strain capable of producing biological surfactant and application thereof
  • Phenol-degrading bacterium strain capable of producing biological surfactant and application thereof
  • Phenol-degrading bacterium strain capable of producing biological surfactant and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Acquisition and identification of strain (Pseudomonas plecoglossicida) BPH-3. The steps of acquisition and identification of bacterial strain (Pseudomonasplecoglossicida) BPH-3 are:

[0019] Step 1. Sample collection and enrichment culture

[0020] The bacteria source was obtained from the activated sludge in the biochemical tank of Wuhan Petrochemical Wastewater Treatment Plant.

[0021] Get 10ml of the activated sludge and place it in an inorganic salt medium with a paraffin concentration of 1vol%, shake it at a constant temperature in a shaker at 150r / min and 30°C, cultivate it for 48h, and then transfer it to a new medium with a paraffin concentration of 1vol%. In the inorganic salt medium, a culture solution was obtained.

[0022] The formula of described inorganic salt culture medium is: (NH 4 ) 2 SO 4 1g, KH 2 PO 4 1g, K 2 HPO 4 1g, NaCl, MgSO 4 2H 2 0 is 0.2g, FeSO 4 ·7H 2 O is 0.02g, anhydrous CaCl 2 0.02g, H 2 O is 1L. The pH value of the mediu...

Embodiment 2

[0030] Effect of initial concentration of phenol on the degradation performance of strain (Pseudomonas plecoglossicida) BPH-3. The bacterial strain (Pseudomonas plecoglossicida) BPH-3 was inoculated into the inorganic salt medium with the initial concentration of phenol being 200, 400, 600, 800, 1000, 1100 mg / L respectively; After culturing in the bed for 12 hours, the effect of the initial concentration of phenol on the degradation performance of strain (Pseudomonas plecoglossicida) BPH-3 was determined. The results are shown in Table 1.

[0031]Table 1 Effect of the initial concentration of phenol on the degradation performance of strain (Pseudomonas plecoglossicida) BPH-3

[0032]

[0033]

[0034] It can be seen from Table 1 that bacterial strain (Pseudomonas plecoglossicida) BPH-3 has a 100% degradation rate to an initial concentration of 200-600 mg / L within 12 hours, and a 100% degradation rate to a phenol initial concentration of 800 mg / L within 18 hours .

[0...

Embodiment 3

[0037] Effect of pH on the degradation of phenol by strain (Pseudomonas plecoglossicida) BPH-3. The bacterial strain (Pseudomonas plecoglossicida) BPH-3 was inoculated into the inorganic salt medium whose initial concentration of phenol was 600 mg / L, and the pH value of the inorganic salt medium was adjusted to 3, 4, 5, 6, 7, 8, 9, 10 and 11, cultivated in a constant temperature shaker at a temperature of 30° C. and a rotation speed of 130 r / min for 12 hours, and measured the influence of pH value on the degradation of phenol by strain (Pseudomonas plecoglossicida) BPH-3. The results are shown in Table 2.

[0038] Table 2 Effect of pH value on degradation of phenol by strain (Pseudomonas plecoglossicida) BPH-3

[0039] pH value

Degradation rate

3

1.35

4

7.49

5

57.94

6

99.45

7

100

8

99.15

9

69.99

10

45.79

11

2.27

[0040] It can be seen from Table 2 that when the pH value is 7, ...

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Abstract

The present invention relates to a phenol-degrading bacterium strain capable of producing a biological surfactant and an application thereof. A technical solution is as follows: the provided bacteriumstrain is pseudomonas plecoglossicida BPH-3 which is preserved by the China Center for Type Culture Collection, a preservation number is: CCTCC NO:M 2019144, and a preservation date is March 14, 2019. A GenBank accession number of 16S rDNA of the pseudomonas plecoglossicida BPH-3 is MK575471, the pseudomonas plecoglossicida BPH-3 is a Gram-negative bacterium strain, and colony is milky white andround, smooth in surface and neat in edges; and after Gram staining, the pseudomonas plecoglossicida BPH-3 is negative under a microscope and presents a short-rod shape. During fermentation degradation, the phenol-degrading bacterium strain forms a glycolipid biological surfactant which can promote solubilization, emulsification, wetting and osmosis of long-chain alkanes and can effectively degrade phenol in coking wastewater and petrochemical wastewater.

Description

technical field [0001] The invention belongs to the technical field of phenol-degrading bacteria. In particular, it relates to a phenol-degrading bacterium producing biosurfactant and its application. Background technique [0002] With the rapid development of petrochemical, coking, plastics and other industries, various phenolic wastewaters have also increased accordingly. Due to the high toxicity of phenols, and it involves the growth and reproduction of aquatic organisms and pollutes water resources, it is necessary to treat phenolic wastewaters. Treatment Take effective measures, and the treatment of phenolic industrial wastewater has become one of the problems to be solved urgently. [0003] Using physical and chemical methods to treat phenolic wastewater may not only cause secondary pollution, but also be uneconomical due to problems such as solvent loss or high energy consumption. The biochemical treatment process is generally simple to operate and low in operating c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C02F3/34C12R1/38C02F101/34
Inventor 李文兵黄慧王光华张政陈铁邓萌
Owner WUHAN UNIV OF SCI & TECH
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