Multiple PCR detection kit for rapidly identifying pathogenic Pseudomonas plecoglossicida, and method thereof

A technology of Pseudomonas ayumi and Pseudomonas, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the lack of specificity and the inability to distinguish virulence pathogens at the same time Strains and non-virulent environmental isolates, etc., to achieve the effect of short detection time, simple equipment requirements, and simple operation
CN103993072AInactive Publication Date: 2014-08-20ZHEJIANG WANLI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG WANLI UNIV
Publication Date
2014-08-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a multiple PCR detection kit for rapidly identifying pathogenic Pseudomonas plecoglossicida, and a method thereof. The kit comprises TaqDNA polymerase, a PCR buffer, a deoxyribonucleoside triphosphate mixture, double distilled water, and primer pairs of Pseudomonadaceae, Pseudomonas plecoglossicida and pathogenic Pseudomonas plecoglossicida. The multiple PCR detection method for rapidly identifying pathogenic Pseudomonas plecoglossicida by using the kit comprises: primary separation of Pseudomonadaceae, and multiple PCR amplifications. Compared with the kit and the method in the prior art, the kit and the method of the present invention have the following advantages that: with application of the kit of the present invention to detect, Pseudomonadaceae, Pseudomonas plecoglossicida and pathogenic Pseudomonas plecoglossicida corresponding to the DNA primer pairs of the three Pseudomonadaceae can be rapidly detected, and with the detection method of the present invention, the presence of the Pseudomonadaceae can be rapidly, sensitively and specifically determined by using the kit, and the pathogenic stain and the non-virulent environment isolation strain can be distinguished.
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Description

technical field

[0001] The invention relates to a detection technology of Pseudomonas ayu, in particular to a multiplex PCR detection kit and a method for rapidly identifying pathogenic Pseudomonas ayu. Background technique

[0002] Pseudomonas species that have been reported to be pathogenic to fish include Pseudomonas anguillacida, P. fluorescens, P. putida, and sweet fish Pseudomonas (P. plecoglossicida), etc., the latter three belong to the DNA type I of Pseudomonas fluorescens, and are similar in morphology and some physicochemical properties, especially Pseudomonas putida and Pseudomonas ayu, These two bacteria belong to the Pseudomonas putida group in terms of taxonomy, and are very similar in physical and chemical properties. The 16S rRNA gene sequence homology is over 99%. Conventional physical and chemical property identification and 16S rRNA gene sequencing comparison are not effective. Distinguish the two bacteria. Pseudomonas ayu NB2011 is a virulent strain th...

Claims

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